Review
Food Science & Technology
Mei Cheng, Hao Wu, Wenli Zhang, Wanmeng Mu
Summary: The production and applications of difructose anhydride III (DFA-III) have attracted considerable attention due to its versatile physiological functions. Recent advances include biosynthetic strategies, purification methods, physiological functions, safety evaluations, current applications, and future research prospects. Enzymatic synthesis of DFA-III has been conducted in both industrial and academic research, with purification achieved through yeast fermentation. Further research should focus on commercial production, comprehensive study of physiological properties, large-scale human experiments, and expansion of industrial applications to explore the potential benefits of DFA-III.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Hanbing Xu, Qiting Li, Wei Zhao, Shuhuai Yu
Summary: In this study, protein engineering was used to improve the activity of DFA-IIIase by modifying amino acids involved in the active site and loop region. Two mutants, E389A and E91K, showed significantly increased activity, 3.63 times and 2.57 times higher than the wild-type enzyme, respectively. This work lays a foundation for the production, further investigation, and application of inulobiose.
PROCESS BIOCHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Masaaki Hanada, Syaw Wynn, Maimaiti Reshalaitihan
Summary: The study found that giving cows DFA III can increase dry matter intake after calving, promote ruminal contraction, and increase milk yield.
Article
Agriculture, Multidisciplinary
Tomonori Unno, Yoshiharu Araki, Shun Inagaki, Makoto Kobayashi, Masaki Ichitani, Takanobu Takihara, Hitoshi Kinugasa
Summary: Dietary fructooligosaccharides (FOS) were found to increase the plasma concentration of EGCG in rats by preventing its auto-oxidation. Treatment with FOS resulted in a dose-dependent increase in cecal lactate levels and alterations in the abundance of Lactobacillus and Collinsella. Consequently, FOS contributed to the protection of EGCG from auto-oxidation and allowed it to be absorbed into the blood circulation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Kenichi Tanabe, Asuka Okuda, Sadako Nakamura, Tsuneyuki Oku
Summary: This study assessed the fermentability of DFA III and MA, showing lower relative available energy compared to fructooligosaccharide.
CYTA-JOURNAL OF FOOD
(2021)
Article
Food Science & Technology
Hanne Vissenaekens, Guy Smagghe, Hanne Criel, Charlotte Grootaert, Katleen Raes, Andreja Rajkovic, Geert Goeminne, Nico Boon, Kristof De Schutter, John Van Camp
Summary: The study demonstrates that quercetin can partially restore mitochondrial dysfunction, especially FCCP-induced dysfunction, in intestinal cells by accumulating in the mitochondria.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Applied
Gaobo Yu, Fengqiong Chen, Yating Wang, Qiusen Chen, Hanlin Liu, Jin Tian, Mengxue Wang, Chunyuan Ren, Qiang Zhao, Fengjun Yang, Yunyan Sheng, Jinpeng Wei, Yuxian Zhang
Summary: This study reveals that GABA effectively improves the nutritional quality of edamame by accumulating amino acids, bioactive peptides, isoflavones, anthocyanins, sugars, and organic acids. Exogenous GABA increases the metabolism of endogenous GABA and enhances the activity and expression of nitrogen metabolism-related enzymes, leading to an accumulation of amino acids and bioactive peptides. Additionally, exogenous GABA induces the metabolism of flavonoids by increasing the activity and expression of flavonoid biosynthetic enzymes.
Article
Biochemistry & Molecular Biology
Yea-eun Lee, Eunok Lee, Urmi Rahman Rinik, Ji Yeon Kim, Byung Hwa Jung, Oran Kwon
Summary: This study investigated the bioavailability of six key polyphenols in Agastache rugosa (Korean mint) and found that rosmarinic acid (RA), acacetin (AC), and tilianin (TA) could be detected in human plasma after ingestion. The transport of RA occurred through the intercellular space, while AC and TA were transported through the epithelial cells. Other AC glucosides with acetyl and/or malonyl groups were rarely found in the plasma. AC glucosides with only an acetyl group appeared at the basolateral side in Caco-2 monolayers, suggesting exclusive hydrolysis of malonyl glucosides in the colon.
Article
Agriculture, Dairy & Animal Science
Topas Wicaksono Priyo Jr, Seiichi Uno, Emiko Kokushi, Katsuki Toda, Hiroshi Hasunuma, Daisaku Matsumoto, Osamu Yamato, Masayuki Ohtani, Oky Setyo Widodo, Dhidhi Pambudi, Masayasu Taniguchi, Mitsuhiro Takagi
Summary: This study compared the serum short-chain fatty acid (SCFA) concentrations in cattle supplemented with DFA III and those in control cattle. The concentrations of isobutyric acid and enanthic acid showed different trends, indicating the fermentation status of DFA III in the intestine. These results suggest that DFA III supplementation may affect the intestinal environment and barrier function in cattle.
Article
Plant Sciences
Chenglei Li, Kai Yang, Jingjing Yang, Huala Wu, Hui Chen, Qi Wu, Haixia Zhao
Summary: This study reveals the important role of FtF3'H1 in anthocyanin and flavonol synthesis in Tartary buckwheat (TB) and provides experimental evidence of its overexpression in Arabidopsis thaliana, Nicotiana tabacum, and TB hairy roots.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Chemistry, Applied
Zhanming Li, Zhongyang Ren, Lin Zhao, Lin Chen, Yue Yu, Dongxu Wang, Xuejin Mao, Guangtian Cao, Zhenlei Zhao, Hongshun Yang
Summary: Advances have been made in understanding the biological effects of dietary flavonoids and flavonoid-rich foods. The role of gut microbiota in their metabolism and health benefits is still unclear. In this study, we explored the types of dietary flavonoids, their low bioaccessibility and metabolic characteristics, the role of gut microbiota in regulation, and the crosstalk between regulation mechanisms. The findings offer new research directions and trends in the development of flavonoid-rich food products.
Article
Agriculture, Multidisciplinary
Siyu Hou, Wei Du, Yanrong Hao, Yuanhuai Han, Hongying Li, Longlong Liu, Kaixuan Zhang, Meiliang Zhou, Zhaoxia Sun
Summary: By investigating the flavonoid metabolism in different TB tissues, the pathway of flavonoid synthesis and the related gene regulation network were revealed. The DEGs were mainly enriched in the Phenylpropanoid biosynthesis pathway, with 52 DEGs involved in flavonol synthesis pathway. Weighted gene correlation network analysis unveiled four co-expression network modules correlated with flavonol metabolites.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Jiarui Jiang, Haitao Huang, Qian Gao, Yong Li, Haiying Xiang, Wanli Zeng, Li Xu, Xin Liu, Jing Li, Qili Mi, Lele Deng, Wenwu Yang, Jianduo Zhang, Guangyu Yang, Xuemei Li
Summary: This study investigates the broad impact of DFR knockout on various developmental phases and organs of tobacco. DFR-KO decreases catabolism and photosynthetic light reactions in tobacco leaves during the flowering stage, while increasing carbon fixation and disease resistance pathways. However, the effects of DFR-KO on tobacco growth progressively decline with time. This research provides valuable insights and theoretical foundations for tobacco breeding and the development of gene-edited strains.
Article
Plant Sciences
Mengying Wang, Biying Dong, Zhihua Song, Meng Qi, Ting Chen, Tingting Du, Hongyan Cao, Ni Liu, Dong Meng, Qing Yang, Yujie Fu
Summary: In this study, it was found that naringenin can enhance the salt tolerance of pigeon pea and affect the changes in flavonoids. Transcriptome analysis identified 13083 differentially expressed genes, which were enriched in metabolic pathways such as phenylpropanoid biosynthesis, starch, and sucrose metabolism. The expression levels of enzyme genes CcIFS, CcCHI, and CcCHS in flavonoid biosynthesis pathway were significantly higher. Transcription factors CcMYB62 and CcbHLH35 were found to be related to flavonoid metabolism, with CcMYB62 showing higher expression level. CcMYB62 induced the upregulation of CcCHI and promoted the accumulation of naringenin, genistein, and biochanin A.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Zhiyuan Li, Hong Jiang, Huizhuan Yan, Xiumei Jiang, Yan Ma, Yong Qin
Summary: The study found that low and moderate levels of nitrogen availability can regulate enzyme activities related to C and N metabolism, promoting the accumulation of carbohydrates, reducing nitrogen metabolite levels, and enhancing the internal balance of C/N ratio. Moreover, under different nitrogen levels, the content of flavonoids in roots and stalks of Coreopsis tinctoria remained stable, while in leaves, it peaked at low or intermediate nitrogen levels. This suggests that nitrogen availability plays a significant role in regulating flavonoid biosynthesis pathways and their relationship with carbon and nitrogen metabolism.
Article
Nutrition & Dietetics
Tohru Hira, Ryoya Suto, Yuka Kishimoto, Sumiko Kanahori, Hiroshi Hara
EUROPEAN JOURNAL OF NUTRITION
(2018)
Article
Biochemistry & Molecular Biology
Masaki Hayakawa, Tohru Hira, Masako Nakamura, Tetsuo Iida, Yuka Kishimoto, Hiroshi Hara
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Nutrition & Dietetics
Jukkrapong Pinyo, Tohru Hira, Hiroshi Hara
Article
Chemistry, Applied
Yosuke Komatsu, Yasuaki Wada, Hirohisa Izumi, Takashi Shimizu, Yasuhiro Takeda, Tohru Hira, Hiroshi Hara
Article
Agriculture, Multidisciplinary
Seiya Tanaka, Aphichat Trakooncharoenvit, Miyu Nishikawa, Shinichi Ikushiro, Hiroshi Hara
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Nutrition & Dietetics
Aphichat Trakooncharoenvit, Seiya Tanaka, Erika Mizuta, Tohru Hira, Hiroshi Hara
EUROPEAN JOURNAL OF NUTRITION
(2020)
Article
Nutrition & Dietetics
Jukkrapong Pinyo, Tohru Hira, Hiroshi Hara
BRITISH JOURNAL OF NUTRITION
(2019)
Article
Medical Laboratory Technology
Akiko Ikuta, Toshihiro Sakurai, Megumi Nishimukai, Yuji Takahashi, Atsushi Nagasaka, Shu-Ping Hui, Hiroshi Hara, Hitoshi Chiba
CLINICA CHIMICA ACTA
(2019)
Letter
Nutrition & Dietetics
Aphichat Trakooncharoenvit, Seiya Tanaka, Erika Mizuta, Tohru Hira, Hiroshi Hara
EUROPEAN JOURNAL OF NUTRITION
(2019)
Review
Endocrinology & Metabolism
Tohru Hira, Jukkrapong Pinyo, Hiroshi Hara
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2020)
Article
Nutrition & Dietetics
Yuki Shimizu, Hiroshi Hara, Tohru Hira
Summary: Although glucose is the most well-known nutrient to stimulate GLP-1 secretion, dietary peptides also play a significant role in stimulating GLP-1 secretion and inhibiting DPP-4 activity. Whey protein administration resulted in less degradation of GLP-1 compared to other nutrients, indicating a potential protective effect of dietary peptides on GLP-1.
BRITISH JOURNAL OF NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Seiya Tanaka, Aphichat Trakooncharoenvit, Miyu Nishikawa, Shinichi Ikushiro, Hiroshi Hara
Summary: Quercetin and methylquercetin are present in various sulfate and glucuronide conjugates in the plasma of quercetin-fed rats and humans. The identification and quantification of different quercetin conjugates in blood plasma, urine, liver, and kidney tissues after oral administration of quercetin were achieved using LC/MS/MS analysis. The study revealed that the profiles of quercetin conjugates varied significantly among different biological samples, indicating selective excretion pathways for these conjugates.
Meeting Abstract
Nutrition & Dietetics
Tohru Hira, Shono Ogasawara, Hiroshi Hara
PROCEEDINGS OF THE NUTRITION SOCIETY
(2020)
Article
Biotechnology & Applied Microbiology
Daiki Mizushima, Takatsugu Miyazaki, Yuh Shiwa, Keitarou Kimura, Shiho Suzuki, Nobuyuki Fujita, Hirofumi Yoshikawa, Atsuo Kimura, Shinichi Kitamura, Hiroshi Hara, Kazumi Funane
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Hiroshi Hara, Shunsuke Kume, Takahisa Iizuka, Yoshinori Fujimoto, Atsuo Kimura
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)