期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 57, 期 21, 页码 10371-10376出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf902614n
关键词
Camellia sinensis; catechins; histochemical localization; vanillin-HCl reagent; staining; HPLC analysis
资金
- National Basic Research Program of China [2007CB116211]
- Natural Science Foundation of China [30771755, 309724111]
- Natural Science Foundation of Anhui Province [090411006]
Histochemical staining using vanillin-HCl is a potential tool to identify the site-specific accumulation of catechins in the tea plant (Camellia sinensis (L.) O. Kuntze). Using this technique revealed that catechins existed ubiquitously in all inspected tissues in young tea leaf, but the distribution was concentrated in the vascular bundle and palisade tissue, whereas the large parenchyma cells of the main vein contained lower amounts of catechins. At the subcellular level, catechins were located mainly in the chloroplasts of mesophyll cells and in the vessel wall. In young stems, catechins could be detected in most cells except the parenchyma cells of the pith and the cortex, whereas, in roots, catechins could be detected only in those cells surrounding the pericycle. Moreover, differing distributions of catechins were found in calluses cultivated in darkness and light. On the basis of HPLC analyses, six main types of catechins were present in tea leaves, stems, calluses, and chloroplasts; however, roots contained only epicatechin.
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