Isolation, Characterization, and Quantification of Steroidal Saponins in Switchgrass (Panicum virgatum L.)

Switchgrass (Panicurn virgatum L.) has been identified for development into an efficient and environmentally friendly biomass energy crop. A recent 5 year study demonstrated that switchgrass grown for biofuel production produced 540% more energy than what is needed to grow, harvest, and process it into cellulosic ethanol. If switchgrass is grown on a scale useful for a bioenergy source, some of the material could be used by livestock as hay or pasture. Switchgrass has been reported to cause hepatogenous photosensitization in lambs (Ovis aries) and horses (Equus caballus). In this study, a simple extraction and rapid reversed phase high-performance liquid chromatography-electrospray ionization-mass spectrometry method was developed for quantifying the major saponins in switchgrass samples. Differences in the relative concentrations of different saponins were observed between switchgrass cultivars and plant parts.