期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 57, 期 6, 页码 2595-2598出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf803395r
关键词
Cyproheptadine; LC-MS/MS; bovine urine
资金
- Spanish Ministerio de Ciencia y Tecnologia [CICYT AGL2005-08073]
- Xunta de Galicia (Conselleria de Agricultura e Politica Agroalimentaria) [PGIDT05TAL20801PR]
The possible off-label and illegal use of cyproheptadine (CYP) as an appetite stimulant for food-producing animals creates the need for methods capable of detecting it. A high-performance liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed to identify CYP in bovine urine, according to Commission Decision 2002/657/EC. Two multiple reaction monitoring (MRM) transitions for each analyte were monitored: 288.1/96.1 and 288.1/191.2 for CYP and 282.1/167.2 and 282.1/116.3 for diphenylpyraline hydrochloride (DPP), which was used as an internal standard. The solid phase extraction technique without a liquid-liquid step gives good results in urine samples from treated animals. The analytical method was successfully validated for linearity (0.15-10 ng/mL), with intraday precision of 9.4%, interday precision of 20.4%, and accuracy of 96.7%. The decision limit (CC alpha) and detection capability (CC beta) were 0.48 and 0.82 ng/mL, respectively.
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