Article
Chemistry, Multidisciplinary
Nikita Orekhov, Nikolay Kondratyuk, Mikhail Logunov, Alexandra Timralieva, Vladimir Shilovskikh, Ekaterina Skorb
Summary: This study provides molecular insights into the self-assembly of melamine cyanurate in aqueous solution and identifies mechanisms of aggregation. The research suggests that the formation of small M-CA complexes is mainly stabilized through aromatic p-p stacking rather than hydrogen bond formation. It also demonstrates that changing the concentration ratio of M to CA leads to a variation in the structure of critical nuclei, indicating a potential for the programmable design of functional supramolecular materials.
CRYSTAL GROWTH & DESIGN
(2021)
Article
Chemistry, Multidisciplinary
Nfayem Imoro, Vladimir V. Shilovskikh, Pavel Nesterov, Alexandra A. Timralieva, Dmitry Gets, Anna Nebalueva, Filipp Lavrentev, Alexander S. Novikov, Nikolay D. Kondratyuk, Nikita D. Orekhov, Ekaterina Skorb
Summary: The study investigates the impact of a dye on the self-assembly process, revealing its crucial role in controlling and regulating the reaction process. The dye is found to influence structure assembly and dye release under both high and low pH conditions. The findings hold promise for applications in bacterial staining and the development of supramolecular capsules.
Article
Environmental Sciences
Aaron J. Pandopulos, Richard Bade, Benjamin J. Tscharke, Jake W. O'Brien, Bradley S. Simpson, Jason M. White, Cobus Gerber
Summary: This study evaluated catecholamine metabolites as potential population biomarkers and found high correlations and similarities in some wastewater catchments in Australia, indicating their suitability as markers for future studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Yi Dai, Chu-Bo Qi, Yang Feng, Qing-Yun Cheng, Fei-Long Liu, Ming-Yu Cheng, Bi-Feng Yuan, Yu-Qi Feng
Summary: The discovery of dynamic and reversible modifications in RNA has expanded their functional repertoires, offering new insight into biological processes. A novel chemical derivatization strategy combined with mass spectrometry analysis has been developed to sensitively and simultaneously determine uridine thiolation and hydroxylation modifications in eukaryotic RNA, enhancing detection sensitivity by up to 339-fold. This method has been used to identify a new modification of 5-hydroxyuridine (ho(5)U) in mammalian cell small RNAs, demonstrating superior capability in detecting low-abundance RNA modifications and potentially leading to the discovery of new modifications in RNA.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Ilya Strashnov, Nuwan Bandara Karunarathna, Biyagamage Ruchika Fernando, Champa Dissanayake, Kasun Madusanka Binduhewa
Summary: The Isotope Dilution Liquid Chromatography-Mass Spectrometry (ID LC-MS) method presented in this study offers a rapid and sensitive detection of melamine in powdered milk, which is particularly beneficial for developing countries with limited laboratory support and surveillance. This method could help improve monitoring of melamine contamination in milk products to ensure consumer safety.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Biochemical Research Methods
Johannes B. Mueller-Reif, Fynn M. Hansen, Lisa Schweizer, Peter Treit, Philipp E. Geyer, Matthias Mann
Summary: Reversed-phase HPLC is commonly used in MS-based proteomics for peptide separation, with particle-packed capillary columns being predominantly used in nanoflow HPLC systems. Despite capillary columns being a standard for many years, they are expensive and have short lifetimes, leading many laboratories to produce their own in-house packed columns.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Chemistry, Analytical
Ge-Ge Gong, Jie Zheng, Sha Li, Ya-Li Bai, Yu-Qi Feng
Summary: A LC-MS method was developed to simultaneously determine 5 RAs in human serum, with enhanced analysis throughput and increased detection sensitivity. The concentrations of three RAs in cancer patient serum samples were significantly decreased compared with health controls, indicating a potential role of RAs in cancer formation and development.
Article
Environmental Sciences
Hangwei Wang, Zhanren Liu, Shaojie Liu, Ruoru Yang, Yifei Wang, Yiying Gu, Min Wu, Ruihua Dong, Bo Chen
Summary: Catechin has potential therapeutic benefits in treating kidney stones induced by co-exposure to MEL and CYA. It significantly reduces crystal deposition and pathological damage, and decreases renal injury markers.
Article
Biochemical Research Methods
Isin T. Sakallioglu, Amith S. Maroli, Aline De Lima Leite, Robert Powers
Summary: This study introduces a rapid reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry method for mycobacterial lipidomics analysis, which achieves separation of various lipid classes specific to mycobacterium in a short runtime of 14 minutes. The method demonstrates high sensitivity and faster sample throughput compared to existing methods.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Pasquale Linciano, Fabiana Russo, Cinzia Citti, Francesco Tolomeo, Roberta Paris, Flavia Fulvio, Nicola Pecchioni, Maria Angela Vandelli, Aldo Lagana, Anna Laura Capriotti, Giuseppe Biagini, Luigi Carbone, Giuseppe Gigli, Giuseppe Cannazza
Summary: The recent discovery of CBDP and Delta 9-THCP has raised questions about their presence and abundance in cannabis, leading to the synthesis of CBDPA and THCPA for quantitative analysis. A targeted analysis of CBD- and THC-type compounds in different cannabis samples was conducted, along with the identification of other heptyl homologs using ultrahigh performance liquid chromatography coupled to high-resolution mass spectrometry.
Article
Environmental Sciences
Sijie Wang, Songdi Wang, Chenxi Wang, Daofu Feng, Xizeng Feng
Summary: Exposure to MCA may disrupt circadian rhythm and thyroid function in adult mice, affecting wheel-running and rest time, hormone homeostasis, gene expression, and thyroid histological morphology.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Emad Karrar, Isam A. Mohamed Ahmed, Thom Huppertz, Fatih Oz, Wei Wei, Xingguo Wang
Summary: This study characterized the triacylglycerols (TAGs) in milk fat from cow, yak, donkey, goat, and camel using UPLC-Q-TOF-MS. A total of 47, 52, 61, 75, and 87 TAG species were identified in camel, goat, donkey, cow, and yak milk fat, respectively. The most abundant TAG species varied among the different milk fats. This work contributes to a better understanding of TAGs in milk from various species.
INTERNATIONAL DAIRY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Jakob Morgenstern, Thomas Fleming, Elisabeth Kliemank, Maik Brune, Peter Nawroth, Andreas Fischer
Summary: This study developed a sensitive and specific method to quantify all-trans retinoic acid (atRA) in human plasma samples, which can also be applied to hyperlipidemic samples. The method showed significant differences in atRA plasma levels between patients with different types of diseases, and the concentration of atRA was associated with lipoprotein levels when compared to healthy controls.
Article
Environmental Sciences
G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: The combination of UV/free chlorine was found to significantly enhance the degradability of VALA compared to other treatments, and was more effective than UV/H2O2 at neutral pH. Three potential transformation products of VALA were identified by LC-QTOF-MS, and the formation of volatile DBPs was observed through gas chromatography.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Xiaopeng Chen, Kyla M. Walter, Galen W. Miller, Pamela J. Lein, Birgit Puschner
BIOMEDICAL CHROMATOGRAPHY
(2018)
Article
Biochemistry & Molecular Biology
Carrie J. Finno, Matthew H. Bordbari, Giuliana Gianino, Brittni Ming-Whitfield, Erin Burns, Janel Merkel, Monica Britton, Blythe Durbin-Johnson, Erica A. Sloma, Marissa McMackin, Gino Cortopassi, Victor Rivas, Marietta Barro, Cecilia K. Tran, Ingrid Gennity, Hadi Habib, Libin Xu, Birgit Puschner, Andrew D. Miller
FREE RADICAL BIOLOGY AND MEDICINE
(2018)
Article
Veterinary Sciences
Megan C. Romano, Alan T. Loynachan, Dave C. Bolin, Uneeda K. Bryant, Laura Kennedy, Mike S. Filigenzi, Birgit Puschner, Robert H. Poppenga, Cynthia L. Gaskill
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2018)
Article
Veterinary Sciences
Carrie J. Finno, Krista E. Estell, Laramie Winfield, Scott Katzman, Matthew H. Bordbari, Erin N. Burns, Andrew D. Miller, Birgit Puschner, Cecilia K. Tran, Libin Xu
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2018)
Article
Engineering, Environmental
Sunjay Sethi, Rhianna K. Morgan, Wei Peng, Yanping Lin, Xueshu Li, Corey Luna, Madison Koch, Ruby Bansal, Michael W. Duffel, Birgit Puschner, R. Thomas Zoeller, Hans-Joachim Lehmler, Isaac N. Pessah, Pamela J. Lein
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2019)
Article
Endocrinology & Metabolism
Kyla M. Walter, Galen W. Miller, Xiaopeng Chen, Bianca Yaghoobi, Birgit Puschner, Pamela J. Lein
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2019)
Article
Environmental Sciences
Lauren Granillo, Sunjay Sethi, Kimberly P. Keil, Yanping Lin, Sally Ozonoff, Ana-Maria Iosif, Birgit Puschner, Rebecca J. Schmidt
ENVIRONMENTAL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Heidi Kucera, Birgit Puschner, Alan Conley, Trish Berger
Article
Veterinary Sciences
Rae A. Sires, Andrea J. Fascetti, Birgit Puschner, Jennifer A. Larsen
TOPICS IN COMPANION ANIMAL MEDICINE
(2019)
Article
Veterinary Sciences
Sabrina N. Hoehne, Casey J. Kohen, Birgit Puschner, Ingrid Gennity, Simon P. Hagley, Kate S. Farrell, Karin Unger, Laura A. Cagle, Karl E. Jandrey
JOURNAL OF VETERINARY EMERGENCY AND CRITICAL CARE
(2019)
Article
Neurosciences
Kyla M. Walter, Galen W. Miller, Xiaopeng Chen, Danielle J. Harvey, Birgit Puschner, Pamela J. Lein
Article
Food Science & Technology
Melissa Bolotaolo, Tomofumi Kurobe, Birgit Puschner, Bruce G. Hammock, Matt J. Hengel, Sarah Lesmeister, Swee J. Teh
Article
Biochemical Research Methods
Gianna Favro, Hadi Habib, Ingrid Gennity, Birgit Puschner, Erin N. Hales, Carrie J. Finno, Benjamin C. Moeller
Summary: An UPLC-APCI-MS/MS method was developed and validated for quantifying vitamin E and its metabolites in equine urine, allowing for evaluation as a diagnostic tool in the treatment of eNAD/EDM. This method demonstrated accuracy and precision in analyzing the concentrations of vitamin E metabolites in healthy horses.
DRUG TESTING AND ANALYSIS
(2021)
Article
Chemistry, Analytical
John P. Buchweitz, Margaret Johnson, Margaret Wixson, Birgit Puschner
Summary: The production and use of methamphetamine is a serious public health problem globally, including in the USA. Accidental or intentional poisoning incidents in companion animals have become common due to the drug's popularity. This case study presents a 4-year-old German Shepherd poisoned by methamphetamine, and discusses the use of postmortem analysis to confirm exposure and the most useful diagnostic specimens for toxicosis in pets.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2022)
Article
Toxicology
Kimberly P. Keil Stietz, Conner L. Kennedy, Sunjay Sethi, Anthony Valenzuela, Alexandra Nunez, Kathy Wang, Zunyi Wang, Peiqing Wang, Audrey Spiegelhoff, Birgit Puschner, Dale E. Bjorling, Pamela J. Lein
Summary: This study indicates that following developmental exposure, PCBs can distribute to the bladder and alter neuroanatomic development and bladder volume in male mice.
CURRENT RESEARCH IN TOXICOLOGY
(2021)