Article
Biochemistry & Molecular Biology
Abhishek Kumar Singh, Boris Schade, Marta Rosati, Rashmi Rashmi, Valentina Dichiarante, Gabriella Cavallo, Pierangelo Metrangolo, Rainer Haag
Summary: Amphiphiles containing fluorinated segments form structures of lower curvature in aqueous solution, which allows for better visualization of their high electron density in cryo-TEM. A modular approach has been developed for the synthesis of nonionic branched amphiphiles, and the aggregation behavior of these structures has been investigated using DLS and cryo-TEM. The results show the formation of multivesicular and multilamellar vesicles, as well as smaller unilamellar vesicles. Furthermore, cell viability tests demonstrate the low toxicity of these nanoarchitectures for potential biomedical applications.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Chemistry, Physical
Xiaoyang Yu, Fan Li, Huasong Fang, Xuyang Miao, Jiyun Wang, Ruowen Zong, Shouxiang Lu
Summary: This study systematically investigated the foaming behavior of a zwitterionic fluorocarbon surfactant used in fire-fighting, revealing differences compared to hydrocarbon surfactants in terms of surfactant adsorption dynamics and solution viscosity. The presence of vesicles formed by FC1157 molecules in solution strongly influences the solution viscosity and foaming ability, highlighting the importance of viscosity and self-assembly structure in determining the foaming behavior.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Applied
Irina Portnaya, Rafail Khalfin, Dganit Danino
Summary: The study found that beta-CN strongly adsorbs onto formed irCN fibrils, likely in the form of micelles, while another amphiphile, Lutrol, also demonstrated adsorption onto the fibrils. Differences were found in the adsorption of the two, with beta-CN adsorbing at higher concentrations after its demicellization/micellization, and Lutrol adsorbing early on during initial injections, indicating suppression of irCN fibrilization and growth by polymer adsorption.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Physical
Mark Gontsarik, Amira Ben Mansour, Linda Hong, Manuel Guizar-Sicairos, Stefan Salentinig
Summary: The study shows that pH-responsive aminolipid self-assemblies can improve the targeted delivery of antimicrobial peptides. Through experiments, it was found that the pH-sensitive nanocarriers based on DODAP/water systems undergo colloidal transformations driven by the protonation of DODAP upon pH decrease. This knowledge can contribute to the design of future pH-responsive AMP delivery systems.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Samuel Watts, Katharina Maniura-Weber, Gilberto Siqueira, Stefan Salentinig
Summary: This study demonstrates pH-responsive interactions of viruses with cationic-modified nanocellulose, creating a filter material that adsorbs nanoscale viruses and can be regenerated by changing the solution's pH. The virus removal capacity of the cationic cellulose-based aerogel filter is 99.9% for MS2 and 93.6% for Qbeta at pH = 7.0.
Article
Polymer Science
Bailee N. N. Barrett, Chi-Huan Tung, Guan-Rong Huang, Istiak Hossain, Chang-Woo Do, Vijay T. T. John, Wei-Ren Chen, Donghui Zhang
Summary: The intramicellar mass heterogeneity of sequence-defined ionic peptoid block copolymers carrying a single charged monomer was studied using contrast variation small-angle neutron scattering analysis. The position of the ionic monomer was found to significantly impact the internal micellar structure. By positioning the ionic monomer closer to the hydrophilic/hydrophobic block junction, the micelles become less compact and show increased chain folding and invasive water. This study demonstrates the potential of using ionic monomer position as a design parameter to control the internal structures of nanoscale micellar assemblies.
Review
Pharmacology & Pharmacy
Na'ama Koifman, Yeshayahu Talmon
Summary: Many pharmaceutical preparations are aqueous dispersions of small or large molecules that self-assemble into complexes, whose efficacy depends on the nanostructure of the complexes. Electron microscopy is necessary to study the nanostructure, but complications arise from the need to make liquid systems compatible with high vacuum in electron microscopes.
Article
Chemistry, Physical
Mark Gontsarik, Anan Yaghmur, Stefan Salentinig
Summary: pH-responsive nanocarriers have the potential to provide targeted delivery of antimicrobial peptides to sites of bacterial infection with abnormal pH levels, and the study demonstrates the tunability of the carriers' pH response range through composition adjustments, advancing our understanding of lipid-peptide self-assembly and pH-responsiveness for future development of adaptable nanocarriers for patient-specific AMP delivery.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Negar Hassani Besheli, Martijn Martens, Elena Macias-Sanchez, Jos Olijve, Fang Yang, Nico Sommerdijk, Sander C. G. Leeuwenburgh
Summary: Through time-resolved analysis and visualization, we elucidated the formation mechanism of gelatin nanoparticles (GNPs), with the ability to precisely control their physicochemical and biofunctional properties by manipulating gelatin molecular weight and type of desolvating agent.
Article
Chemistry, Applied
Mo Li, Xin Wen, Kunli Wang, Zihao Liu, Yuanying Ni
Summary: This study reported the improvement of stability of 8-CN micelles by Maillard-induced glycation reaction using dextran as a modification agent. The results showed that glycation enhanced the solubility of 8-CN-graft-dextran at acidic pH and formed stable micelles with irregular surfaces. This finding has potential for future applications of modified 8-CN-graft-dextran as a stable nanocarrier.
Review
Polymer Science
Lei Guo, Junting Xu, Binyang Du
Summary: Multiblock copolymers with more complex block composition and sequences have gained increasing scientific attention. The ABCBA linear pentablock terpolymer serves as a representative model for studying the self-assembly of multiblock copolymers, providing insights into the assembled structures and solution properties of biomacromolecules with complex sequence structures. Compared to AB diblock copolymers and ABC triblock terpolymers, ABCBA pentablock terpolymers exhibit more intricate morphologies and improved mechanical properties. This review focuses on the recent research progress in the self-assembly of ABCBA linear pentablock terpolymers in bulk and solution, as well as their potential applications in various technical fields including drug carriers, surface coatings, inorganic material templates, battery materials, and polymer gel.
Article
Chemistry, Applied
Ruslan Kashapov, Yuliya Razuvayeva, Albina Ziganshina, Syumbelya Amerhanova, Anastasiia Sapunova, Alexandra Voloshina, Vadim Salnikov, Lucia Zakharova
Summary: This study demonstrates the use of amphiphilic viologen calix[4]resorcinol for the spontaneous co-aggregation with sodium caseinate, resulting in the formation of functional nanoparticles. These nanoparticles can solubilize both hydrophobic and hydrophilic molecules without the use of organic solvents, and exhibit cytotoxic and penetrating properties.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Physical
Laura Fioretto, Marcello Ziaco, Carmela Gallo, Genoveffa Nuzzo, Giuliana d'Ippolito, Pietro Lupetti, Eugenio Paccagnini, Mariangela Gentile, Marina DellaGreca, Marie-Sousai Appavou, Luigi Paduano, Raffaele De Palma, Angelo Fontana, Emiliano Manzo
Summary: This study investigates the chemical characteristics and self-assembly behavior of Sulfavant A and Sulfavant R, two potential vaccine adjuvants. The results demonstrate that the formation and stability of their aggregates vary with concentration, which in turn affects their immunomodulatory abilities.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Tatijana Markoska, Davor Daniloski, Todor Vasiljevic, Thom Huppertz
Summary: The study found that temperature and pH significantly influenced the secondary structure of beta-casein, particularly affecting random coils and alpha-helical structures. Changes in structural components were associated with decreased hydrophobic interactions at lower temperature and increasing pH. The most noticeable alteration in the alpha-helix occurred when the pH was adjusted to 7.0 and the temperature set at 4°C, leading to disruption of hydrogen bonds and weakening of hydrophobic interactions.
Article
Chemistry, Physical
Prakram Singh Chauhan, Inbal Abutbul Ionita, Hen Moshe Halamish, Alejandro Sosnik, Dganit Danino
Summary: The use of antiretroviral (ARV) cocktail therapy has revolutionized the treatment of HIV and shows promise for other infectious diseases. This study demonstrates a new encapsulation technology platform for oral delivery of fixed dose combinations, enhancing their physicochemical stability and release profiles.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Physical
J. Rodrigo Magana, Maria Homs, Jordi Esquena, Inbar Freilich, Ellina Kesselman, Dganit Danino, Carlos Rodriguez-Abreu, Conxita Solans
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2019)
Article
Nanoscience & Nanotechnology
Prakram Singh Chauhan, Murali Kumarasamy, Alejandro Sosnik, Dganit Danino
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Multidisciplinary Sciences
Mingming Zhang, Doron Grossman, Dganit Danino, Eran Sharon
NATURE COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Srinivas Ramishetti, Inbal Hazan-Halevy, Ramesh Palakuri, Sushmita Chatterjee, Somu Naidu Gonna, Niels Dammes, Inbar Freilich, Luba Kolik Shmuel, Dganit Danino, Dan Peer
ADVANCED MATERIALS
(2020)
Article
Chemistry, Physical
Prakram Singh Chauhan, Inbal Abutbul Ionita, Hen Moshe Halamish, Alejandro Sosnik, Dganit Danino
Summary: The use of antiretroviral (ARV) cocktail therapy has revolutionized the treatment of HIV and shows promise for other infectious diseases. This study demonstrates a new encapsulation technology platform for oral delivery of fixed dose combinations, enhancing their physicochemical stability and release profiles.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Judit Tomsen-Melero, Solene Passemard, Natalia Garcia-Aranda, Zamira Vanessa Diaz-Riascos, Ramon Gonzalez-Rioja, Jannik Nedergaard Pedersen, Jeppe Lyngso, Josep Merlo-Mas, Edgar Cristobal-Lecina, Jose Luis Corchero, Daniel Pulido, Patricia Camara-Sanchez, Irina Portnaya, Inbal Ionita, Simo Schwartz Jr, Jaume Veciana, Santi Sala, Miriam Royo, Alba Cordoba, Dganit Danino, Jan Skov Pedersen, Elisabet Gonzalez-Mira, Ibane Abasolo, Nora Ventosa
Summary: The incorporation of cationic miristalkonium chloride (MKC) surfactant into RGD nanovesicles has been shown to improve loading capacity and colloidal stability, enhancing the protection and delivery of the enzyme. Using MKC in two different nanosystems increases the stability of enzyme loading, with low concentrations of MKC in hybrid liposomes stabilizing the enzyme without compromising its activity. Hybrid liposomes also show improved efficacy in cell cultures and a good safety profile, ensuring their potential for future preclinical and clinical development.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Kathrin Siegl, Luba Kolik-Shmuel, Mingming Zhang, Sylvain Prevost, Kalanit Vishnia, Amram Mor, Marie-Sousai Appavou, Charl J. Jafta, Dganit Danino, Michael Gradzielski
Summary: Monodisperse unilamellar nanotubes and nanoribbons were transformed into multilamellar structures using a step-wise approach. This process, verified by small-angle neutron scattering and cryogenic-transmission electron microscopy, allows for the controlled formation of multi-walled suprastructures with specific layer spacing and surface charge. It has potential applications in life science and nanotechnology.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Physical
Josep Merlo-Mas, Judit Tomsen-Melero, Jose-Luis Corchero, Elisabet Gonzalez-Mira, Albert Font, Jannik N. Pedersen, Natalia Garcia-Aranda, Edgar Cristobal-Lecina, Marta Alcaina-Hernando, Rosa Mendoza, Elena Garcia-Fruitos, Teresa Lizarraga, Susanne Resch, Christa Schimpel, Andreas Falk, Daniel Pulido, Miriam Royo, Simo Schwartz, Ibane Abasolo, Jan Skov Pedersen, Dganit Danino, Andreu Soldevila, Jaume Veciana, Santi Sala, Nora Ventosa, Alba Cordoba
Summary: Fabry disease is a lysosomal storage disease caused by a deficiency of the enzyme alpha-galactosidase A (GLA). This study optimized the preparation of alpha-galactosidase-loaded nanoliposomes for the treatment of Fabry disease using a Quality by Design approach, identifying critical parameters through risk analysis and Design of Experiments to achieve a stable nanoformulation suitable for in vivo preclinical testing.
JOURNAL OF SUPERCRITICAL FLUIDS
(2021)
Article
Chemistry, Multidisciplinary
Guy Jacoby, Merav Segal Asher, Tamara Ehm, Inbal Abutbul Ionita, Hila Shinar, Salome Azoulay-Ginsburg, Ido Zemach, Gil Koren, Dganit Danino, Michael M. Kozlov, Roey J. Amir, Roy Beck
Summary: This study introduces a new type of peptide amphiphiles, intrinsically disordered peptide amphiphiles (IDPA), that exhibit a sharp pH-induced micellar phase-transition. The shape transition can serve as a mechanism for the design of cargo hold-and-release applications, demonstrating the potential of tailoring interactions between disordered peptides for various stimuli-responsive biomedical applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Materials Science, Biomaterials
Prakram Singh Chauhan, Murali Kumarasamy, Angel M. Carcaboso, Alejandro Sosnik, Dganit Danino
Summary: This study encapsulated pitavastatin (PTV) in silica-coated polymeric micelles surface-modified with a cyclic peptide targeting the integrin receptors to potentially treat GBM. The silica shell provided colloidal stability and controlled release kinetics, showing efficacy in inducing apoptosis in glioma cells and reducing cell viability. Overall, the results suggest the potential of these hybrid nanocarriers for targeted therapy of GBM and other integrin receptor-overexpressing tumors.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Uri Elia, Shahar Rotem, Erez Bar-Haim, Srinivas Ramishetti, Gonna Somu Naidu, David Gur, Moshe Aftalion, Ma'ayan Israeli, Adi Bercovich-Kinori, Ron Alcalay, Efi Makdasi, Theodor Chitlaru, Ronit Rosenfeld, Tomer Israely, Sharon Melamed, Inbal Abutbul Ionita, Dganit Danino, Dan Peer, Ofer Cohen
Summary: The COVID-19 pandemic led to the development of mRNA vaccines, and a recent study demonstrated that an RBD-hFc mRNA vaccine could provide protection against lethal SARS-CoV-2 infection in mice expressing human ACE2. The vaccinated mice showed robust humoral responses and 70% of them survived the lethal virus challenge, while all control animals succumbed to infection.
Article
Chemistry, Applied
Irina Portnaya, Rafail Khalfin, Dganit Danino
Summary: The study found that beta-CN strongly adsorbs onto formed irCN fibrils, likely in the form of micelles, while another amphiphile, Lutrol, also demonstrated adsorption onto the fibrils. Differences were found in the adsorption of the two, with beta-CN adsorbing at higher concentrations after its demicellization/micellization, and Lutrol adsorbing early on during initial injections, indicating suppression of irCN fibrilization and growth by polymer adsorption.
FOOD HYDROCOLLOIDS
(2021)
Article
Nanoscience & Nanotechnology
Yael Cohen, Roi Rutenberg, Guy Cohen, Boris Veltman, Raanan Gvirtz, Elazar Fallik, Dganit Danino, Evgeni Eltzov, Elena Poverenov
ACS APPLIED BIO MATERIALS
(2020)
Article
Chemistry, Physical
Guy Jacoby, Irina Portnaya, Dganit Danino, Haim Diamant, Roy Beck