期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 56, 期 8, 页码 2624-2629出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf072888h
关键词
Avena sativa L.; oat bran sulfated; beta-glucan; potent anti-HIV activity; low cytotoxicity
China is a major producer of oats; the annual harvested area of 350,000 ha yields approximately 465,000 tons, giving an average yield of 1.33 tons/ha. The bran is not used for animal feed as it is of poor digestibility and low nutritive content and is considered a waste byproduct. Therefore, it is advantageous to produce a value-added product from the bran. We extracted the native polysaccharide, a linear (1-3)-, (1-4)-linked beta-glucan (OBG) from the oat bran and synthesized a sulfated derivative OBGS containing 36.5% sulfate. OBGS had potent activity against a primary isolate of human immunodeficiency virus (HIV-1) in peripheral blood mononuclear cells at a concentration (EC50 = 5.98 x 10(-4) mu M) approximately 15,000 times below its cytotoxic concentration. OBGS was also active postinfection (EC50 = 5.3 x 10(-4) mu M) and protected pretreated peripheral mononuclear cells (EC50 = 5.2 X 10(-2) mu M) washed free of the compounds prior to infection. Thus, OBGS has potential as a vaginal microbicide and is the first such report for oat bran derived sulfatea beta-glucan.
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