Article
Genetics & Heredity
Shamila Weerakoon Abeynayake, Sonia Fiorito, Adrian Dinsdale, Mark Whattam, Bill Crowe, Kate Sparks, Paul Richard Campbell, Cherie Gambley
Summary: DNA barcoding sequencing provides a rapid and accurate method for identifying invertebrate pests at the border, supporting morphological identification and enabling more confident identification of immature stages and eggs. Sequencing the COI barcode region using ONT MinION technology proves to be a cost-effective and field-adaptable approach for rapid and accurate identification.
Article
Microbiology
Luis Solorzano Alava, Cesar Bedoya Pilozo, Hilda Hernandez Alvarez, Lazara Rojas Rivera, Misladys Rodriguez Ortega, Jorge Fraga Nodarse, Leandro de Mattos Pereira, Raquel de Oliveira Simoes, Roberto do Val Vilela
Summary: The study aimed to investigate the genetic diversity of Angiostrongylus cantonensis in Ecuador. The analysis revealed that all A. cantonensis isolates from different provinces in Ecuador were genetically identical and clustered with isolates from the USA and Asia. This suggests that the invasion of A. cantonensis in Ecuador may have occurred recently and originated from Asia through the USA. Further research should include samples from neighboring countries.
Article
Entomology
Romana Iftikhar, Amalendu Ghosh, Hanu R. Pappu
Summary: This study investigated the genetic variation and evolutionary parameters of Thrips tabaci populations in the United States. The results revealed high genetic diversity and structure of the onion thrips, which is a significant pest that causes severe damage and acts as a vector of plant viruses.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yvan Papa, Pierre-Yves Le Bail, Raphael Covain
Summary: This study utilized various methods to classify and identify fish species in the Maroni Basin, resulting in the discovery of 25 new candidate species and revealing more cryptic diversity. Genetic divergence showed significant differences between different regions, possibly due to lower levels of connectivity. A new method of ordination of genetic landscapes helped effectively categorize species based on their patterns of genetic divergence.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Biology
Giang Tran Thi, Ilenia Azzena, Fabio Scarpa, Piero Cossu, Cong Danh Le, Phuong Anh Ton Nu, Thi Minh Chau Ngo, Daria Sanna, Marco Casu
Summary: This study investigated the genetic variation of Taenia saginata in central regions of Vietnam using the mitochondrial gene COI. The results revealed a founder effect in Southeast Asia and a common haplotype shared among many countries except Laos, which shares its most common haplotype only with individuals from Thailand.
Article
Biotechnology & Applied Microbiology
Xianhui Wang, Huannan Li, Xueling Zhan, Mingyang Ma, Danni Yuan, Qiang Hu, Yingchun Gong
Summary: A quantitative real-time polymerase chain reaction (qPCR) method was developed to detect and quantify P. malhamensis in high-density Chlorella cultures, successfully applied for monitoring its population dynamics in open ponds and detecting its wide distribution in the environment.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Veterinary Sciences
Bahador Hajimohammadi, Abdolhossein Dalimi, Gilda Eslami, Salman Ahmadian, Sajad Zandi, Ahmad Baghbani, Saeedeh Sadat Hosseini, Vahideh Askari, Maryam Sheykhzadegan, Mehrnoosh Nabizadeh Ardekani, Mohammad Javad Boozhmehrani, Mohammad Javad Ranjbar, Hamed Ghoshouni, Mahmood Vakili
Summary: This study analyzed the hydatid cysts in livestock slaughtered in an abattoir in Central Iran and found that the G1-G3 and G6/G7 genotypes were present in all animals, while G5 was only reported in cattle, goats, and camels. This is the first molecular identification of cystic echinococcosis in Central Iran, highlighting the need to consider the reporting of G5 in livestock in this area due to its potential transmission to humans.
BMC VETERINARY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Riho Gross, Leona Lovrencic, Misel Jelic, Frederic Grandjean, Simona Duretanovic, Vladica Simic, Oksana Burimski, Lena Bonassin, Marius-Ioan Groza, Ivana Maguire
Summary: The noble crayfish in the western part of the Balkan Peninsula exhibit significant genetic diversity, with new divergent mitochondrial haplotypes identified. Microsatellite data suggests that these populations harbor an important component of genetic diversity within A. astacus. The results indicate the role of the western Balkans as microrefugia during Pleistocene climate fluctuations, allowing for long term persistence of A. astacus populations in the region.
Article
Evolutionary Biology
Stanislav Ozana, Ales Dolny, Tomas Panek
Summary: This study discusses the risks and limitations of using mitochondrial markers for taxonomic identification and biodiversity monitoring in Odonata, revealing that NUMTs can have a serious impact on molecular studies of Odonata, especially when the barcode is located in the cox1 gene. It is suggested that nad1 should be used alone or in combination with cox1 to minimize unintended confusion with NUMTs.
SYSTEMATIC ENTOMOLOGY
(2022)
Article
Fisheries
Chenxia Zuo, Tingting Zhang, Chenchen Zhang, Daotan Zhao, Yi Zhu, Xiaojie Ma, Haiyan Wang, Peizhen Ma, Zhen Zhang
Summary: This study tested the utility of five gene fragments for analyzing the genetic diversity of Mytella strigata populations in China. The results showed that COI was the most suitable gene fragment for genetic diversity studies, while D1 28S and 18S-ITS1 were useful for species identification. The study also revealed that the likely origin of M. strigata in China is Colombia, and the invasive populations in China have experienced or are currently experiencing a population bottleneck.
Article
Evolutionary Biology
James S. Evans, Patrick M. Erwin, Hendra F. Sihaloho, Susanna Lopez-Legentil
Summary: This study analyzed color morphs, genetic lineages, and symbiotic microbial communities of the colonial ascidian D. bermudensis, finding that two distinct genetic lineages corresponded to unique color morphs and microbial communities, indicating that two different species coexist under the same scientific name. The study highlights the utility of symbiont profiling in resolving the taxonomic status of phenotypically variable ascidian species.
Article
Ecology
Kazumasa Hashimoto, Katsumasa Yamada, Masashi Sekino, Mayumi Kobayashi, Takenori Sasaki, Yuichiro Fujinami, Masayuki Yamamoto, Kwang-Sik Choi, Yasuhisa Henmi
Summary: The study revealed the genetic structure and diversity of the pen shell in East Asia, finding that the species is composed of six monophyletic lineages, with lineages 1 and 2 having a wide distribution range. Hierarchical AMOVA results showed significant genetic differentiation among sampling site groups, possibly due to post-recruitment selection or coastal topography.
REGIONAL STUDIES IN MARINE SCIENCE
(2021)
Article
Entomology
Esther Lantero, Beatriz Matallanas, M. Dolores Ochando, Carmen Callejas
Summary: This study analyzed over 250 fruit flies from six different Mediterranean countries, providing relevant data on the genetic structure and gene flow of the olive fruit fly. These findings are important for improving integrated pest management strategies.
Article
Biology
Fevzi Bardakci, Sarah Hilan Mohammed Al-Subaie, Riadh Badraoui, Mohd Adnan, Arif Jamal Siddiqui
Summary: The present study used the mitochondrial barcoding gene COI to identify and characterize hard ticks infesting camels in Ha'il province, Saudi Arabia. Nine haplotypes were found, with six being newly described. Phylogenetic analysis revealed two distinct clades corresponding to H. dromedarii and H. impeltatum. The presence of H. scupense together with H. impeltatum suggests possible asymmetrical hybridization and mitochondrial introgression between these species.
Article
Engineering, Marine
Bo Liu, Yali Yan, Nan Zhang, Huayang Guo, Baosuo Liu, Jingwen Yang, Kecheng Zhu, Dianchang Zhang
Summary: Species markers can be assessed quickly and accurately using DNA barcoding. In this study, we used DNA barcoding to investigate the parrotfish family Scaridae in Hainan, and found that DNA barcoding can be an effective tool for monitoring, protecting, and managing fisheries, as well as for addressing taxonomic issues.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)