Article
Food Science & Technology
Thibault Caron, Melanie Le Piver, Anne-Claire Peron, Pascale Lieben, Rene Lavigne, Sammy Brunel, Daniel Roueyre, Michel Place, Pascal Bonnarme, Tatiana Giraud, Antoine Branca, Sophie Landaud, Christophe Chassard
Summary: Studies on the domestication of food microorganisms can provide valuable insights into adaptation mechanisms and applications. Penicillium roqueforti, a fungus with four genetically distinct populations, has different impacts on the characteristics of blue cheeses when strains from different populations are used for inoculation, leading to variations in flavor and appearance.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Guifang Xie, Ying Lu, Wenkai Li, Zhenyun He, Zhichang Sun, Xiaoxia Xie, Xing Liu
Summary: Antibody affinity and enzyme loads are critical factors in determining the sensitivity of enzyme-linked immunosorbent assay for small molecular contaminants in food. This study successfully improved the antibody affinity and enzyme loads by utilizing the strategy of multimerization, and developed an enhanced colorimetric enzyme immunoassay for ochratoxin A (OTA).
Article
Biochemistry & Molecular Biology
Diane M. Spencer, Angel Garza Reyna, David S. Pisetsky
Summary: DNA can have different backbone conformations, with Z-DNA being a left-handed helix with a zig-zag orientation depending on base sequence, modification, and supercoiling. Experimental results demonstrate that anti-Z-DNA antibodies can serve as probes for detecting the presence of Z-DNA in DNA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Meng Bai, Cai-Juan Zheng, Guang-Ying Chen
Summary: Three unusual austins-type meroterpenoids were isolated from a mangrove-derived fungus, each with unique structural features. These compounds showed inhibitory effects on the growth of Helicoverpa armigera larvae.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Multidisciplinary Sciences
Beatriz Alvarez-Rodriguez, Javier Buceta, Ron Geller
Summary: Despite limited understanding, a comprehensive analysis reveals significant variation in the neutralization sites targeted by polyclonal antibody responses to non-enveloped viruses in individual humans. This knowledge helps predict viral escape and identify key structural and evolutionary parameters. A minimum of two mutations is required for significant escape from multiple sera.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Applied
Xun Yang, Zhichang Sun, Zhenyun He, Xiaoxia Xie, Xing Liu
Summary: Mimotope-based immunoassays enable the use of smaller amounts of mycotoxin standards for artificial antigen synthesis. In this study, nanobody-based magnetic beads were used to screen the mimotope of ochratoxin A (OTA) from a peptide library. Two novel immunoassay platforms, PN-ELISA and APN-ELISA, based on nanobody and peptidomimetic were developed and showed good analytical performances for detecting OTA in food.
Article
Food Science & Technology
Janne Leivo, Markus Vehniainen, Urpo Lamminmaki
Summary: The use of synthetic antibody libraries and phage displays allows for efficient generation of specific antibodies against a wide range of targets, particularly those that are difficult to target. In this study, anti-idiotype antibodies with unique binding properties were successfully generated from synthetic antibody libraries for deoxynivalenol mycotoxin, leading to the development of specific assays for its detection.
Article
Microbiology
Ruiling Zhuo, Yong Chen, Mengyang Xing, Zhanquan Zhang, Shiping Tian, Boqiang Li
Summary: In this study, it was found that Penicillium expansum is a main producer of patulin that causes severe postharvest decay and food safety issues in the fruit industry. The development, pathogenicity, and patulin production of P. expansum are strongly influenced by the PacC-pH signaling pathway. The PeEna family proteins, as important downstream targets of PePacC, play a crucial role in patulin biosynthesis in P. expansum.
Review
Agriculture, Multidisciplinary
Dan-Tong Huang, Hui-Jun Fu, Jia-Jia Huang, Lin Luo, Hong-Tao Lei, Yu-Dong Shen, Zi-Jian Chen, Hong Wang, Zhen-Lin Xu
Summary: Mycotoxins are toxic contaminants in foods and feeds that are naturally occurring and largely unavoidable. Immunoassays are well-established for mycotoxins due to their speed, sensitivity, simplicity, and cost-effectiveness. The use of mimotopes, such as mimic peptides and anti-idiotypic antibodies, can provide alternative methods for rapid analysis of mycotoxins without the requirement of toxic mycotoxins.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Holly P. Bartholomew, Dianiris Luciano-Rosario, Michael J. Bradshaw, Verneta L. Gaskins, Hui Peng, Jorge M. Fonseca, Wayne M. Jurick, Essaid Ait Barka, Tomislav Cernava, Beibei Ge
Summary: This study investigates two Penicillium chrysogenum isolates as potential biocontrol agents against Penicillium expansum in apples. The results show that these isolates can effectively reduce decay incidence and severity in apples, without showing antagonism with Penicillium expansum. These findings contribute to the development of novel decay intervention strategies for managing postharvest diseases.
Article
Chemistry, Applied
Ines Rodriguez-Canas, Jesus M. Gonzalez-Jartin, Rebeca Alvarino, Amparo Alfonso, Mercedes R. Vieytes, Luis M. Botana
Summary: This study presents a reliable method to quantify 32 mycotoxins in cheese. The method involves a QuEChERS extraction process and UHPLC-MS/MS detection. The method was validated for accuracy and precision, and applied to the analysis of 38 commercial cheese samples. High occurrences of beauvericin and enniatins were found, with ochratoxin A concentrations posing a potential risk to human health in three samples.
Review
Biotechnology & Applied Microbiology
Clare Mills, Katrina Campbell
Summary: There is an increasing demand for rapid and affordable in-field screening methods for low molecular weight (LMW) compound detection. This review examines the recent resurgence of using anti-idiotypes (Ab2s) and the potential role of phage display and nanobodies in improving LMW compound immunoassays.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Prashant Ashok Shelke, Latha Sabikhi, Yogesh Khetra, Sangita Ganguly, Davuddin Baig
Summary: The study found that using a lower proportion (90:10) of skim milk and higher temperature (95 degrees C) in the heat treatment during cow milk Ricotta cheese production results in better quality cheese. Principal component analysis can group cheeses based on the level of cheese milk and heating temperature.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Sofia Lepesioti, Evangelia Zoidou, Dionysia Lioliou, Ekaterini Moschopoulou, Golfo Moatsou
Summary: The study found that heating conditions inducing denaturation of beta-lactoglobulin and leaving native alpha-lactalbumin were suitable for making clean-label Quark-type cheese, while homogenization was more effective for full-fat cheese. Treatment of whey proteins resulted in harder, springier, and less cohesive cheese, with effects varying based on heat treatment intensity and homogenization. Storage had minimal impact on composition and texture but did affect specific chemical components of the cheese.
Review
Engineering, Environmental
Saowalak Adunphatcharaphon, Christopher T. Elliott, Thanasat Sooksimuang, Ratthaphol Charlermroj, Awanwee Petchkongkaew, Nitsara Karoonuthaisiri
Summary: Mycotoxins pose serious threats to public health, the economy, and the environment. The co-contamination of multiple hazardous mycotoxins in commodities and the environment adds complexity to the problem. This review discusses emerging rapid immuno-based multiplex methods for detecting mycotoxins in agricultural products and feed ingredients published within the past five years, including lateral flow, fluorescence polarization, chemiluminescence, surface plasmon resonance, surface enhanced Raman scattering, electrochemical sensor, and nanoarray. The future trend of detection methods for multiple mycotoxins is predicted to rely on advancements in sensor technologies, nanomaterial-based enhancing and reporting signals, rapid sample preparation, and quantitative analysis capacity.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Food Science & Technology
Ulas Acaroz, Richard Dietrich, Maria Knauer, Erwin Maertlbauer
FOOD ANALYTICAL METHODS
(2020)
Article
Multidisciplinary Sciences
Daniel Fox, Anukriti Mathur, Yansong Xue, Yunqi Liu, Wei Hong Tan, Shouya Feng, Abhimanu Pandey, Chinh Ngo, Jenni A. Hayward, Ines I. Atmosukarto, Jason D. Price, Matthew D. Johnson, Nadja Jessberger, Avril A. B. Robertson, Gaetan Burgio, David C. Tscharke, Edward M. Fox, Denisse L. Leyton, Nadeem O. Kaakoush, Erwin Maertlbauer, Stephen H. Leppla, Si Ming Man
NATURE COMMUNICATIONS
(2020)
Article
Food Science & Technology
Nadja Jessberger, Richard Dietrich, Kristina Schauer, Stefanie Schwemmer, Erwin Maertlbauer, Roland Benz
Article
Food Science & Technology
Valerie Schwenk, Janina Riegg, Monique Lacroix, Erwin Maertlbauer, Nadja Jessberger
Review
Food Science & Technology
Richard Dietrich, Nadja Jessberger, Monika Ehling-Schulz, Erwin Maertlbauer, Per Einar Granum
Summary: Bacillus cereus is a soil bacterium responsible for two types of food-related gastrointestinal diseases: food intoxication and food infections. The diseases are caused by various toxins produced by the bacterium, including cereulide and proteinaceous enterotoxins. The review covers the distribution, genetic organization, regulation, and secretion mechanisms of these toxins, with a focus on Hbl and Nhe enterotoxin complexes.
Editorial Material
Food Science & Technology
Erwin Maertlbauer, Per Einar Granum
Article
Virology
Marie-T. Koenig, Robert Fux, Ellen Link, Gerd Sutter, Erwin Maertlbauer, Andrea Didier
Summary: The detection of circular DNA elements in milk samples from domesticated Asian water buffaloes is a rapidly evolving area in virology, with implications for higher incidence of colorectal and breast cancer in certain regions. The genomes obtained show similarity to BMMF sequences, previously isolated from commercial milk, and have characteristic features such as rep genes, tandem repeats, and inverted repeats. This finding highlights the importance of considering circular ssDNA in the study of cancer hypothesis.
Article
Biochemistry & Molecular Biology
Kristina J. H. Kleinwort, Bernhard F. Hobmaier, Ricarda Mayer, Christina Hoelzel, Roxane L. Degroote, Erwin Martlbauer, Stefanie M. Hauck, Cornelia A. Deeg
Summary: Proteomic analysis of Mycobacterium avium subspecies paratuberculosis (MAP) cultivated in milk revealed upregulation of proteins involved in metabolism, fatty acid metabolism/synthesis, stress responses, immune evasion, transcription/translation, and bacterial cell wall synthesis. Proteins such as Dut, MmpL4_1, and RecA were only detected in MAP incubated in milk, suggesting crucial roles in coping with a stressful environment. Additionally, 35 candidates with stable expression in all conditions were identified as potential detection targets.
Article
Virology
Marie-Therese Koenig, Robert Fux, Ellen Link, Gerd Sutter, Erwin Maertlbauer, Andrea Didier
Summary: Recently, various CRESS DNA viruses and unclassified virus-like DNA elements have been discovered in a wide range of animal species and environmental samples. The presence of these entities, especially BMMF, in dairy products such as sheep and goat milk, suggests a potential impact on human health. This study highlights the importance of further research on the dissemination and potential risks associated with these pathogens in human nutrition.
Article
Multidisciplinary Sciences
Valerie Schwenk, Richard Dietrich, Andreas Klingl, Erwin Maertlbauer, Nadja Jessberger
Summary: This study delves into the strain-specific variations in Bacillus cereus swimming motility by generating polyclonal rabbit antisera and monoclonal antibodies, as well as developing a highly specific sandwich EIA. The presence of flagella/flagellin titres obtained in EIAs was found to correlate with strain-specific swimming motility. Additionally, a specific monoclonal antibody was able to inhibit bacterial swimming motility.
Article
Immunology
Nadja Jessberger, Richard Diedrich, Robert Janowski, Dierk Niessing, Erwin Maertlbauer
Summary: This study confirmed the widespread expression of Hbl B' in Bacillus cereus and its important regulatory function in balancing the formation of complexes and the amounts of free subunits.
Article
Biochemistry & Molecular Biology
Kristina J. H. Kleinwort, Maria Weigand, Lydia Hoffmann, Roxane L. Degroote, Richard Dietrich, Erwin Maertlbauer, Stefanie M. Hauck, Cornelia A. Deeg
Summary: Recently, there have been stability issues with dairy products with extended shelf life, resulting in significant liquefaction. This project identified the enzymes responsible for this liquefaction through proteomic analysis. These findings are useful for adjusting production protocols and additives to extend the shelf life of these products.
Article
Biochemistry & Molecular Biology
Lucia Korbonits, Kristina J. H. Kleinwort, Barbara Amann, Andrea Didier, Erwin Martlbauer, Stefanie M. Hauck, Cornelia A. Deeg
Summary: This study aimed to detect proteomic changes in cows with different MAP infection status after co-incubation with viable MAP in vitro. The results showed that MAP-resistant cows exhibited enriched immune-related signaling pathways, indicating a successful defensive immune response to MAP. On the other hand, persistently MAP-infected cows did not show enrichment in immune-related signaling pathways. These distinct immune responses contribute to a better understanding of the bovine immune response and susceptibility mechanisms to MAP.
Article
Agriculture, Dairy & Animal Science
Marie-Therese Koenig, Kai Froelich, Anabell Jandowsky, Tobias Knauf-Witzens, Christoph Langner, Richard Dietrich, Erwin Maertlbauer, Andrea Didier
Summary: Circular replicase-encoding single-stranded (CRESS) DNA viruses and other circular DNA agents were detected in bovids from African and Asian cattle, with the majority belonging to the BMMF groups 1 and 2, and a smaller part being CRESS viruses. This study provides new insights into the diversity and presence of circular DNA in ruminants used for food production worldwide.
Article
Chemistry, Applied
I Csadek, M. Wagner, D. Lingnau, T. Alter, D. Herrfurth, D. Meemken, V Oswaldi, R. Isbrandf, N. Langkabel, C. Kehrenberg, R. Stephan, M. Morach, S. Johler, W. Zens, A. S. Braun, E. Maertlbauer, B. Sperner, M. Koethe, N. Sudhaus-Joern, C. Krischek, M. Ploetz, H. Mitterer-Istyagin, S. Birka, J. Saffaf, T. Albert, P. G. Braun
Summary: Due to the pandemic, universities had to adjust their teaching methods. The veterinary universities/faculties in German-speaking countries established a common teaching objectives catalogue to ensure consistent and efficient teaching. The summary of the first semester during the pandemic showed that e-learning elements had great potential for teaching.
JOURNAL OF FOOD SAFETY AND FOOD QUALITY-ARCHIV FUR LEBENSMITTELHYGIENE
(2022)