期刊
JOURNAL OF AFFECTIVE DISORDERS
卷 133, 期 3, 页码 646-654出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jad.2011.04.044
关键词
Bipolar disorder; Brain-derived neurotrophic factor; Cerebellum; Major depression; Schizophrenia; TrkB
资金
- U. S. National Institutes of Health (NIH) [MH62512, MH83506, DA26306, DA12065, DA27513, DA26334, MH79881, MH79728, MH74508, MH81482, MH78728, MH76681, MH81861, MH85560]
- neuroAIDS [MH62512, MH83506, DA26306, MH81482, HNRC889]
Background: The role of the cerebellum in coordinating mental activity is supported by its connections with cerebral regions involved in cognitive/affective functioning, with decreased activities on functional neuroimaging observed in the cerebellum of schizophrenia patients performing mental tasks. Brain-derived neurotrophic factor (BDNF)-induced activation of tyrosine kinase B (TrkB) is essential to synaptic plasticity. We hypothesized that alterations in BDNF and TrkB expression in the cerebellum were associated with schizophrenia and affective disorders. Methods: We employed immunohistochemistry and immunoblotting to quantify protein expression of BDNF and TrkB in the cerebellum of patients with schizophrenia, bipolar disorder, and major depression compared to controls (n = 15 each). Results: While TrkB immunoreactivity in each of the molecular and granule-cell layers was reduced in all 3 disease groups (12-34%) compared to the control (P=0.018 and 0.038, respectively, ANOVA), only the reduction in bipolar disorder remained statistically significant upon Tukey-Kramer post hoc analyses (P= 0.019 and 0.021, respectively). Apparent decreases in BDNF immunoreactivity in all 3 disease groups (12-30%) compared to the control were not statistically significant. TrkB immunoreactivity was not significantly associated with any of the demographic, clinical, and postmortem variables. Immunoblotting displayed an 85-kDa TrkB-immunoreactive band, consistent with a truncated isoform, in all 60 cases. Limitations: On immunoblotting. apparent decreases in 85-kDa-TrkB levels in all 3 disease groups compared to the control were not statistically significant. Conclusions: Our finding of reduced TrkB expression in bipolar disorder suggests that dysregulation of TrkB-mediated neurotrophin signaling in the cerebellum may play a role in the pathophysiology of this disease. (C) 2011 Elsevier B.V. All rights reserved.
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