期刊
MONATSHEFTE FUR CHEMIE
卷 147, 期 2, 页码 465-471出版社
SPRINGER WIEN
DOI: 10.1007/s00706-015-1520-x
关键词
Enzymes; Nanostructures; Magnetic properties; Fluorescence spectroscopy
资金
- University of Kashan
- University of Shahrekord
The goal of this work was investigation the effect of adsorption the Fe3O4 as magnetic nanoparticle on lysozyme activity and thermal stability. The turbidimetric assay (activity) of lysozyme was followed by the decreased optical density of a turbid cell suspension (about 0.3 mg/cm(3) Micrococcus lysodeikticus) photometrically at 450 nm. In this study, the effect of nano-Fe3O4 on lysozyme activity was investigated by UV-Vis spectrophotometry at pH = 7.25 at 35 A degrees C using sodium phosphate buffer. Measurements were carried out using 6 x 10(-8) M (0.1 mg/cm(3)) concentration of lysozyme and a range of nano-Fe3O4 concentrations between 0 and 0.06 mg/cm(3). It was found that by increasing nano-Fe3O4, the rate of M. lysodeikticus lyses (lysozyme activity) increases. The thermal stability of lysozyme was investigated in the presence of nano-Fe3O4 over the temperature range of 293-363 K in sodium phosphate buffer and pH = 7.25. Results indicated that by increasing nano-Fe3O4, the thermal stability of lysozyme increases. [GRAPHICS] .
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据