Article
Immunology
Teena Mohan, Ha T. Nguyen, Krista Kniss, Vasiliy P. Mishin, Angiezel A. Merced-Morales, Jennifer Laplante, Kirsten St George, Patricia Blevins, Anton Chesnokov, Juan A. De la Cruz, Rebecca Kondor, David E. Wentworth, Larisa Gubareva
Summary: Four cases of oseltamivir-resistant influenza A(H1N1) pdm09 virus infection were detected among inhabitants of a border detention center in Texas, USA. The hemagglutinin of these viruses belongs to subclade 6B.1A5A-156K, which may enable viral escape from preexisting immunity, highlighting the importance of monitoring both drug resistance and antigenic drift of circulating viruses.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Biology
Harry L. Stannard, Edin J. Mifsud, Steffen Wildum, Sook Kwan Brown, Paulina Koszalka, Takao Shishido, Satoshi Kojima, Shinya Omoto, Keiko Baba, Klaus Kuhlbusch, Aeron C. Hurt, Ian G. Barr
Summary: A clinical influenza isolate with dual antiviral resistant mutations shows a reduced viral fitness in ferrets, indicating that widespread community transmission of this dual mutant is unlikely.
COMMUNICATIONS BIOLOGY
(2022)
Article
Virology
Omid Rashidi, Afagh Moattari, Neda Pirbonyeh, Amir Emami, Mohammad Rahim Kadivar, Nahid Tavakoli Movaghar, Fahimeh Edalat
Summary: The study aimed to determine the genetic mutation of the neuraminidase gene in influenza A (H1N1) viruses in southwest Iran. The emergence of oseltamivir resistance was identified, highlighting the importance of conducting similar evaluations in different geographical regions and planning treatment strategies for influenza.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Weixu Zhang, Hefeng Xu, Shuxuan Guan, Chengmin Wang, Guoying Dong
Summary: This study analyzed the available NA protein sequences of H1N1 influenza viruses worldwide and found that 3.76% of H1N1 viruses currently harbor oseltamivir resistance, with the most common mutation being H274Y. Oseltamivir resistance is mainly found in humans, swine, and avian, and the resistance level peaked in 2007-2008 and quickly decreased in 2009. The study highlights the importance of globally monitoring oseltamivir resistance.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Editorial Material
Medicine, General & Internal
Mohammad Danyal Kayani
Summary: Swine flu, caused by H1N1 influenza virus, is commonly transmitted from animals, especially pigs, to humans. While most cases are mild to moderate and non-fatal, we report a rare case of a healthy woman from Pakistan who died from a severe course of H1N1 influenza virus despite aggressive treatment with Osaltemivir and antibiotics. The emergence of Osaltemivir resistance in many countries, including Pakistan, highlights the importance of surveillance and antiviral resistance testing in third world countries.
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION
(2022)
Review
Immunology
Frederick G. Hayden, Jason Asher, Benjamin J. Cowling, Aeron C. Hurt, Hideyuki Ikematsu, Klaus Kuhlbusch, Annabelle Lemenuel-Diot, Zhanwei Du, Lauren Ancel Meyers, Pedro A. Piedra, Takahiro Takazono, Hui-Ling Yen, Arnold S. Monto
Summary: Studies indicate that prompt antiviral treatment can reduce the risk of influenza virus transmission to contacts, especially in household settings. However, reported effects vary widely across studies. Additionally, all approved classes of influenza antivirals carry a potential risk of transmitting drug-resistant variants.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Critical Care Medicine
Abhishek Deshpande, Michael Klompas, Pei -Chun Yu, Peter B. Imrey, Andrea M. Pallotta, Thomas Higgins, Sarah Haessler, Marya D. Zilberberg, Peter K. Lindenauer, Michael B. Rothberg
Summary: This study investigates the frequency and timing of influenza testing and its associations with treatment and outcomes in adults hospitalized with community-acquired pneumonia (CAP). The results show that a significant proportion of CAP patients are not tested for influenza, even during flu season. However, for patients testing positive, they receive antiviral treatment more often and early treatment is associated with lower mortality and shorter hospital stays.
Article
Biotechnology & Applied Microbiology
Vaclav Brazda, Otilia Porubiakova, Alessio Cantara, Natalia Bohalova, Jan Coufal, Martin Bartas, Miroslav Fojta, Jean-Louis Mergny
Summary: The study identified multiple potential G-quadruplex forming sequences in the G4-EA-H1N1 genomes, which could serve as potential therapeutic targets.
Article
Immunology
Chenyi Xie, Wen Su, Sin Fun Sia, Ka-Tim Choy, Steven Morrell, Jie Zhou, Malik Peiris, Jesse D. Bloom, Hui-Ling Yen
Summary: This study identified that A(H1N1)pdm09 influenza viruses are mainly transmitted via airborne particles produced by infected ferrets' upper respiratory epithelial cells. The findings highlight the importance of reducing viral loads in the upper respiratory tract with vaccines and antiviral drugs to prevent influenza transmission.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Renee W. Y. Chan, Kin P. Tao, Jiqing Ye, Kevin K. Y. Lui, Xiao Yang, Cong Ma, Paul K. S. Chan
Summary: Influenza virus is a major public health challenge characterized by high morbidity and mortality. Oseltamivir is commonly used as an antiviral, but resistant strains have been detected. This study designed and synthesized two new compounds that showed significant antiviral activity against both seasonal and oseltamivir-resistant strains. These compounds have the potential to be an important addition to the arsenal of antiviral drugs.
Article
Multidisciplinary Sciences
Maki Kiso, Yuri Furusawa, Ryuta Uraki, Masaki Imai, Seiya Yamayoshi, Yoshihiro Kawaoka
Summary: In this study, the authors characterized mutant viruses with reduced sensitivity to nirmatrelvir, an oral antiviral agent that targets SARS-CoV-2. They found that these mutant viruses showed a slower growth rate and attenuated phenotypes in infection models. However, it is important to closely monitor the emergence of nirmatrelvir-resistant SARS-CoV-2 variants as they could potentially become dominant.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
Yogesh Sharma, Chris Horwood, Paul Hakendorf, Campbell Thompson
Summary: The study showed that early administration of Oseltamivir in adult hospitalized influenza patients can reduce 30-day readmissions and the composite outcome of 30-day readmissions and mortality.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemical Research Methods
Haokun Yuan, Sarah C. Kramer, Eric H. Y. Lau, Benjamin J. Cowling, Wan Yang
Summary: Climate drivers such as humidity and temperature have a significant impact on influenza transmission in subtropical regions, with a simple model effectively replicating epidemic patterns observed in Hong Kong by quantifying the bimodal effect of absolute humidity on transmission and the nonlinear relationship with temperature. Shorter immunity periods also play a crucial role in approximating the co-circulation of influenza virus (sub)types at the population level. The study's findings are consistent with laboratory studies on influenza survival and transmission dynamics under different humidity and temperature levels.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Microbiology
Junyi Hu, Zhe Hu, Yandi Wei, Ming Zhang, Senlin Wang, Qi Tong, Honglei Sun, Juan Pu, Jinhua Liu, Yipeng Sun
Summary: This study suggests that SIVs with H1N1/2009 genotypes could undergo rapid adaptive changes in a new host, especially in the PB2 and HA genes, which may facilitate the emergence of pandemic strains such as H1N1/2009. Mutations like PB2D309N and HAL425M were found to enhance the replication and pathogenicity of rH1N1 in mice and enable its transmission in other mammals.
VETERINARY MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yusuke Kato, Kazuhiro Takahashi, Fuyu Ito, Shoichi Suzuki, Kiyoshi Fukui, Masakazu Mimaki, Kazuo Suzuki
Summary: This study identified oseltamivir-resistant influenza B samples with common amino acid substitutions that decrease the affinity of oseltamivir to neuraminidase. These substitutions, N340D and E358K, located far from the binding site, rearranged hydrogen bond networks and altered the electrostatic potential map, indirectly influencing affinity through novel mechanisms. These findings may inform the development of drugs for treating oseltamivir-resistant virus infections.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Surgery
H. F. Wunderink, E. van der Meijden, C. S. van der Blij-de Brouwer, M. J. K. Mallat, G. W. Haasnoot, E. W. van Zwet, E. C. J. Claas, J. W. de Fijter, A. C. M. Kroes, F. Arnold, A. Touze, F. H. J. Claas, J. I. Rotmans, M. C. W. Feltkamp
AMERICAN JOURNAL OF TRANSPLANTATION
(2017)
Article
Immunology
Marjolein J. Korndewal, Anne Marie Oudesluys-Murphy, Aloys C. M. Kroes, Ann C. T. M. Vossen, Hester E. de Melker
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2017)
Article
Immunology
Roberta Rovito, Frans H. J. Claas, Geert W. Haasnoot, Dave L. Roelen, Aloys C. M. Kroes, Ann C. T. M. Vossen
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2018)
Article
Public, Environmental & Occupational Health
Rebecca van Grootveld, Manu P. Bilsen, Timo L. Boelsums, Edou R. Heddema, Geert H. Groeneveld, Jairo Gooskens, Mark G. J. de Boer
VECTOR-BORNE AND ZOONOTIC DISEASES
(2018)
Article
Immunology
Roberta Rovito, Frans H. J. Claas, Geert W. Haasnoot, Dave L. Roelen, Aloys C. M. Kroes, Michael Eikmans, Ann C. T. M. Vossen
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Multidisciplinary Sciences
Elisabeth M. Terveer, Roel H. T. Nijhuis, Monique J. T. Crobach, Cornelis W. Knetsch, Karin E. Veldkamp, Jairo Gooskens, Ed J. Kuijper, Eric C. J. Claas
Article
Virology
Aline L. van Rijn, Herman F. Wunderink, Igor A. Sidorov, Caroline S. de Brouwer, Aloysius C. M. Kroes, Hein Putter, Aiko P. J. de Vries, Joris Rotmans, Mariet C. W. Feltkamp
Summary: The dynamics of TTV load predict allograft rejection in kidney transplantation recipients, but not BKPyV and CMV infections. Further validation is needed to explore the potential use of TTV load levels as a guide for optimal immunosuppressive drug dosage to prevent allograft rejection.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Microbiology
Maria M. Konstantinovski, Karin Ellen Veldkamp, Adriana P. M. Lavrijsen, Thijs Bosch, Margriet E. M. Kraakman, Sam Nooij, Eric C. J. Claas, Jairo Gooskens
Summary: Staphylococcus aureus is a major cause of hospital infections worldwide. While there is high awareness towards MRSA infections, attention towards BORSA is limited. Research shows that BORSA has a higher incidence among patients, carrying multiple resistance and virulence genes, highlighting the importance of timely implementation of infection control measures.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Virology
Alhena Reyes, Ellen C. Carbo, Johan S. van Harinxma Thoe Slooten, Margriet E. M. Kraakman, Igor A. Sidorov, Eric C. J. Claas, Aloys C. M. Kroes, Leo G. Visser, Jutte J. C. Vries De
Summary: Viral metagenomics can help detect viral pathogens and co-infections in international travellers with febrile illness, and the use of probes for enrichment results in high genome coverage and depth.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Microbiology
Ellen C. Carbo, Anne Russcher, Margriet E. M. Kraakman, Caroline S. de Brouwer, Igor A. Sidorov, Mariet C. W. Feltkamp, Aloys C. M. Kroes, Eric C. J. Claas, Jutte J. C. de Vries
Summary: In this study, the mNGS approach was used for the identification and load monitoring of transplantation-related DNA viruses in immunocompromised patients. The results showed that the mNGS method performed comparably to qPCR in detection and viral load determination.
Article
Microbiology
Maria M. M. Konstantinovski, Leo M. M. Schouls, Sandra Witteveen, Eric C. J. Claas, Margriet E. E. Kraakman, Jayant Kalpoe, Eva Mattson, David J. J. Hetem, Erika P. M. van Elzakker, Jos Kerremans, Vishal Hira, Thijs Bosch, Jairo Gooskens
Summary: This study assessed the genetic epidemiology and clinical characteristics of Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in an urban area with a limited livestock population in the Netherlands. The findings suggest that LA-MRSA may cause a significant burden of disease in urban areas, with most infections occurring through inter-human transmission rather than livestock exposure.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Alieke van der Hoeven, Martha T. van der Beek, Vincent Bekker, Erin Meijers, Marco J. R. Ivens, Els Wessels, Aloysius C. M. Kroes, Stefan A. Boers
Summary: Bacterial meningitis in infants is a life-threatening condition, and conventional bacterial culture may not always detect the causative microorganisms. The addition of 16S rRNA sequencing greatly improves the identification of bacterial meningitis.
INFECTIOUS DISEASES AND THERAPY
(2023)
Letter
Immunology
Jairo Gooskens, Marja M. Konstantinovski, Margriet E. M. Kraakman, Jayant S. Kalpoe, Nathalie D. van Burgel, Eric C. J. Claas, Thijs Bosch, Haga Ziekenhuis
Summary: This study reports the detection of Panton-Valentine leukocidin-positive clonal complex 398 human-origin methicillin-resistant Staphylococcus aureus L2 in the Netherlands. This hyper-virulent lineage, originating from the Asia-Pacific Region, has the potential to become community-acquired in Europe through recurrent travel-related introductions. Genomic surveillance plays a crucial role in early detection, guiding control measures, and limiting pathogen spread in urban settings.
EMERGING INFECTIOUS DISEASES
(2023)
Letter
Infectious Diseases
L. B. S. Aulin, C. I. M. Koumans, Y. Haakman, W. van Os, M. E. M. Kraakman, J. Gooskens, W. K. Smits, A. Liakopoulos, J. G. C. van Hasselt
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Medicine, General & Internal
K. C. E. Sigaloff, P. K. Chung, J. Koopmans, D. W. Notermans, G. G. C. van Rijckevorsel, M. Koene, R. W. Sprengers, J. Gooskens, J. E. Stalenhoef
NETHERLANDS JOURNAL OF MEDICINE
(2017)