期刊
ISME JOURNAL
卷 7, 期 9, 页码 1752-1763出版社
SPRINGERNATURE
DOI: 10.1038/ismej.2013.62
关键词
oxidase; respiration; Shewanella
资金
- Major State Basic Research Development Program (973 Program) [2010CB833803]
- Natural Science Foundation of Zhejiang province [R3110096]
- Major Program of Science and Technology Department of Zhejiang province [2009C12061]
- National Natural Science Foundation of China [31270097]
Shewanella species are a group of facultative Gram-negative microorganisms with remarkable respiration abilities that allow the use of a diverse array of terminal electron acceptors (EA). Like most bacteria, S. oneidensis possesses multiple terminal oxidases, including two heme-copper oxidases (caa(3)-and cbb(3)-type) and a bd-type quinol oxidase. As aerobic respiration is energetically favored, mechanisms underlying the fact that these microorganisms thrive in redox-stratified environments remain vastly unexplored. In this work, we discovered that the cbb(3)-type oxidase is the predominant system for respiration of oxygen (O-2), especially when O-2 is abundant. Under microaerobic conditions, the bd-type quinol oxidase has a significant role in addition to the cbb(3)-type oxidase. In contrast, multiple lines of evidence suggest that under test conditions the caa(3)-type oxidase, an analog to the mitochondrial enzyme, has no physiological significance, likely because of its extremely low expression. In addition, expression of both cbb(3)-and bd-type oxidases is under direct control of Crp (cAMP receptor protein) but not the well-established redox regulator Fnr (fumarate nitrate regulator) of canonical systems typified in Escherichia coli. These data, collectively, suggest that adaptation of S. oneidensis to redox-stratified environments is likely due to functional loss of the caa(3)-type oxidase and switch of the regulatory system for respiration.
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