Article
Microbiology
Jasmin Frey, Sophie Kassner, Dieter Spiteller, Mario Mergelsberg, Matthias Boll, David Schleheck, Bernhard Schink
Summary: The study showed that sulfate-reducing bacteria degrade butanone, isopropanol, and acetone through TDP-dependent formylation, using the same enzymes for degradation.
Article
Agriculture, Multidisciplinary
Jian He, Wenjing Qiao, Junwei Liu, Shiyu Zhao, Ningning Wu, Gang Hu, Jiguo Qiu
Summary: In this study, a sulfate-reducing bacterium SRB-5 was isolated and identified as Cupidesulfovibrio sp. It was found that SRB-5 possesses a novel anaerobic pathway capable of degrading acetochlor. The study also demonstrated that SRB-5 could degrade other chloroacetamide herbicides, highlighting its potential application in remediation of contaminated sites.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Environmental Sciences
Jinlong Gao, Yifan Chen, Jiwei Li, Yalin Yu, Jun Wang, Tiantian Pang, Yuting Qi, Jingge Shang, Qianjiahua Liao
Summary: This study investigated the treatment of 100-mg/L Norfloxacin (NOR) wastewater containing high concentrations of sulfate through a combination of electrolysis, sulfate-reducing up-flow sludge bed (SRUSB), and biological contact oxidation reactor (BCOR) treatments. The results showed that electrolysis transformed most of the NOR, while SRUSB and BCOR played important roles in degrading TOC and SO42? contents. Sulfate-reducing bacteria and sulfate-oxidizing bacteria were crucial in carbon chain oxidation and benzene ring opening during the treatment process.
ENVIRONMENTAL RESEARCH
(2021)
Article
Microbiology
Qiuyun Jiang, Hongmei Jing, Hao Liu, Mengran Du
Summary: This study investigated microbial communities in cold seep and trough sediments in the South China Sea and observed differences in biogeography and gene abundance. The study found closely related genotypes in wetland and mangrove microbial communities and higher relative abundance of the NC10 group in the Xisha trough. The study highlights the importance of combining functional genes and 16S rRNA genes to understand population shifts in natural microbial communities among different oceanic regions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Qiuyun Jiang, Hongmei Jing, Xuegong Li, Ye Wan, I-Ming Chou, Lijun Hou, Hongpo Dong, Yuhui Niu, Dengzhou Gao
Summary: This study reveals the microbial diversity and potential activities of DAMO and SAMO processes in deep-sea cold seeps using isotopic tracing incubations and provides direct evidence for the occurrence of Nr-/N-DAMO as a previously overlooked microbial methane sink in deep-sea hydrate-bearing sediments.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jing Wang, Xiaohong Li, Fang Guan, Zhibo Yang, Xiaofan Zhai, Yimeng Zhang, Xuexi Tang, Jizhou Duan, Hui Xiao
Summary: In this study, culturable sulfate-reducing bacteria (SRB) were isolated and identified from the inner rust layer of steel and sediments. A comparison of amino acid sequences encoding related enzymes in the sulfate reduction pathway was conducted between anaerobic and facultative anaerobic SRB strains. The study found differences in the distribution of enzymes between the two types of SRB, providing insights into the metabolic mechanisms and potential applications of SRB.
Review
Engineering, Chemical
Ivan Kushkevych, Aneta Kovarova, Dani Dordevic, Jonah Gaine, Peter Kollar, Monika Vitezova, Simon K. -M. R. Rittmann
Summary: This review discusses the importance of sulfate-reducing bacteria (SRB) in producing hydrogen sulfide and their cryopreservation methods. It highlights the factors affecting microbial viability after cryopreservation and recommends storing SRB in glass vials with low salt concentration. Furthermore, it emphasizes the potential impact of SRB in medical, construction, and environmental sectors from a molecular biological and bioengineering perspective.
Article
Engineering, Environmental
Ginevra Giangeri, Panagiotis Tsapekos, Maria Gaspari, Parisa Ghofrani-Isfahani, Marie Karen Tracy Hong Lin, Laura Treu, Panagiotis Kougias, Stefano Campanaro, Irini Angelidaki
Summary: The presence of sulfate in anaerobic digestion systems reduces methane yield, as sulfate-reducing bacteria compete with methanogens for substrates. This study demonstrates that the addition of magnetite can enhance methane production and reduce hydrogen sulfide levels. Furthermore, genomic analysis reveals both competitive and cooperative interactions between methanogens and sulfate-reducing bacteria, depending on the specific methanogenic group.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Oceanography
Huan Zhang, Junming Zhou, Peng Yuan, Yanhui Dong, Wenxiao Fan, Xing Yu, Jianggu Lu
Summary: Mn-rich sediments were discovered at the active Haima cold seeps in the South China Sea, from which Mn-micronodules were extracted. These micronodules were rapidly formed through biomineralization and chemical precipitation, and were supplied with sufficient Mn by metal-rich seepage fluids. Their presence demonstrates manganese bacterial activities and indirectly reflects metal-dependent anaerobic methane oxidation.
JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS
(2023)
Article
Engineering, Environmental
Zuotao Zhang, Jiao Sun, Xiaoqiang Gong, Chongyang Wang, Hui Wang
Summary: A new sulfate-reducing bacterium, Desulforamulus aquiferis strain DSA, was found to degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) pyrene and benzo[a]pyrene, while reducing sulfate to sulfide. The metabolic processes for anaerobic pyrene and benzo[a]pyrene degradation were proposed based on detecting intermediates and analyzing the genome. This study provides new guidance for anaerobic HMW-PAHs degradation under sulfate-reducing conditions.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Ecology
Kartik Dhar, Logeshwaran Panneerselvan, Suresh R. Subashchandrabose, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Summary: This study reports on the enrichment culture of Desulfotomaculum, which plays a key role in the anaerobic degradation of PAHs, under completely anaerobic sulfate-reducing conditions.
Article
Biochemistry & Molecular Biology
Pavel Stahel, Vera Mazankova, Daniela Podzemna, Erika Podzemna, Veronika Pizurova, Jana Jurmanova, Lubomir Prokes, Marian Lehocky, Kadir Ozaltin, Hana Pistekova, David Trunec
Summary: Antibacterial polymeric thin coatings were deposited on teflon substrates using atmospheric pressure plasma polymerization. The films showed high antibacterial activity against both S. aureus and E. coli strains, while remaining cytocompatible.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Febrian Hillman, Mohamad Rezi Abdul Hamid, Panagiotis Krokidas, Salvador Moncho, Edward N. Brothers, Ioannis G. Economou, Hae-Kwon Jeong
Summary: The delayed linker addition (DLA) synthesis strategy allows for efficient incorporation of Im linkers into ZIF-8 framework, improving gas separation performance. The Im/ZIF-8 synthesized via DLA demonstrates outstanding performance in gas separation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biotechnology & Applied Microbiology
Samuel C. Eziuzor, Felipe B. Correa, Shuchan Peng, Junia Schultz, Sabine Kleinsteuber, Ulisses N. da Rocha, Lorenz Adrian, Carsten Vogt
Summary: This study investigated the metabolism of benzene by microbes under anoxic conditions. The results showed that benzene mineralization was correlated with the enrichment of a particular Peptococcaceae phylotype, and observed a slight increase in other abundant taxa. Despite benzene being the sole organic carbon source, the microbial community remained diverse, suggesting complex interactions between different functional groups.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Asma Wasfi, Mawahib Sulieman, Ziad Sefelnasr, Abdulla Alteneiji, Atawulrahman Shafiqurrahman, Ammar Alharairi, Falah Awwad
Summary: Gas sensing is an important research area in aerospace, military, medical, and industrial environments for preventing risks caused by toxic gases. This study introduces the use of two-dimensional nitrogenated holey graphene (C2N) sensors for identifying butane and propane gases. The sensors, enhanced with gold electrodes and a gate terminal, detect the gases through measuring their effect on electrical conductivity. Through advanced simulations, the C2N-based sensors show high sensitivity and accuracy, offering potential for small size and low-cost gas sensing applications.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Marc Tamisier, Matthias Schmidt, Carsten Vogt, Steffen Kuemmel, Hryhoriy Stryhanyuk, Niculina Musat, Hans-Hermann Richnow, Florin Musat
Summary: The research focused on the stable carbon and hydrogen isotope effects associated with methane formation by the corrosive archaeon Methanobacterium strain IM1 during growth with hydrogen and iron. The growth resulted in methane with depleted C-13 values, while H-2 values were less impacted by growth phase. The corrosion crusts formed were composed of siderite, calcite, and iron sulfide, with attached cells found on crust surfaces and deep inside crust domes.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Federica Calabrese, Hryhoriy Stryhanyuk, Cristina Moraru, Michael Schloemann, Lukas Y. Wick, Hans H. Richnow, Florin Musat, Niculina Musat
Summary: This study investigates the dynamics of anabolic heterogeneity in microbial populations under nutrient replete conditions, revealing two major trends of the heterogeneity over time. The results suggest an interplay of metabolic history and metabolic fitness in shaping the heterogeneity of microbial populations during substrate assimilation.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Johan Decelle, Giulia Veronesi, Charlotte LeKieffre, Benoit Gallet, Fabien Chevalier, Hryhoriy Stryhanyuk, Sophie Marro, Stephane Ravanel, Remi Tucoulou, Nicole Schieber, Giovanni Finazzi, Yannick Schwab, Niculina Musat
Summary: Photosymbiosis between colonial Collodaria and their microalga dinoflagellate Brandtodinium was investigated using multimodal subcellular imaging. The study revealed a dynamic symbiosis where symbionts interact with host cells via extracellular vesicles. Results showed that the photosynthetic apparatus of the microalgae was more voluminous in symbiosis, with carbon and nitrogen stored in starch granules and purine crystals in the symbiotic microalga. The study sheds light on the biogeochemical significance of this oceanic photosymbiosis.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Ecology
Song-Can Chen, Rohit Budhraja, Lorenz Adrian, Federica Calabrese, Hryhoriy Stryhanyuk, Niculina Musat, Hans-Hermann Richnow, Gui-Lan Duan, Yong-Guan Zhu, Florin Musat
Summary: This study successfully characterized uncultured microorganisms responsible for the aerobic degradation of biphenyl in the environment and revealed their significant role in pollutant degradation.
Article
Multidisciplinary Sciences
Clarisse Uwizeye, Margaret Mars Brisbin, Benoit Gallet, Fabien Chevalier, Charlotte LeKieffre, Nicole L. Schieber, Denis Falconet, Daniel Wangpraseurt, Lukas Schertel, Hryhoriy Stryhanyuk, Niculina Musat, Satoshi Mitarai, Yannick Schwab, Giovanni Finazzi, Johan Decelle
Summary: This study reveals that cell division of endosymbionts is blocked within hosts, leading to radical changes in cellular structure and bioenergetic machinery; photosynthetic efficiency increases with cell size, and networked mitochondrial architecture enhances light capture; hosts trigger and benefit from major bioenergetic remodeling of symbiotic microalgae.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Genetics & Heredity
Rima M. Chakaroun, Lucas Massier, Anna Heintz-Buschart, Nedal Said, Joerg Fallmann, Alyce Crane, Tatjana Schuetz, Arne Dietrich, Matthias Blueher, Michael Stumvoll, Niculina Musat, Peter Kovacs
Summary: The study found that the bacterial signature in the blood is related to metabolic diseases and can be improved after bariatric surgery. This work provides contaminant-aware evidence for the presence of living bacteria in the blood and suggests a putative crosstalk between components of the blood and metabolism in metabolic health regulation.
Article
Materials Science, Multidisciplinary
T. Malyi, V Tsiumra, V. Vistovskyy, N. Mitina, N. Musat, H. Stryhanyuk, A. Kondyr, A. Zaichenko, A. Voloshinovskii
Summary: Template synthesis with heterofunctional oligoperoxide was used to obtain inorganic nanoparticles of controlled size, which showed reduced aggregation and higher luminescence efficiency even at high temperatures.
Article
Microbiology
Song-Can Chen, Jiaheng Ji, Denny Popp, Ulrike Jaekel, Hans-Hermann Richnow, Stefan M. Sievert, Florin Musat
Summary: The metabolic potential of the sulfate-reducing bacterium Desulfosarcina sp. strain BuS5, capable of oxidizing propane and butane without oxygen, was investigated. Genomics, proteomics, and physiology assays revealed that BuS5 encodes an alkyl-succinate synthase that initiates the oxidation of both propane and butane, with the resulting alkyl-succinates being oxidized to CO2. BuS5 conserves energy through sulfate reduction and electron bifurcation. However, its ability to utilize long-chain fatty acids, mannose, and oligopeptides, suggested by annotation pipelines, was not supported by experimental findings. These findings highlight BuS5 as a metabolic specialist, capable of growing only with propane and butane.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Endocrinology & Metabolism
Rima Chakaroun, Lucas Massier, Niculina Musat, Peter Kovacs
Summary: Despite the ongoing debate, recent studies have shown that under strict contamination-free conditions, various methods combined with advanced bioinformatics tools can detect and quantify bacteria and bacterial DNA in human tissues. Changes in bacterial load and composition in the blood have been associated with several disease states, potentially contributing to systemic inflammation in cardiometabolic disease. This review explores the clinical relevance of a blood bacterial signature to cardiometabolic diseases and proposes strategies for future research and application, considering the challenges posed by low bacterial biomass.
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES
(2022)
Article
Multidisciplinary Sciences
Nestor Arandia-Gorostidi, Hugo Berthelot, Federica Calabrese, Hryhoriy Stryhanyuk, Isabell Klawonn, Morten Iversen, Nurun Nahar, Hans-Peter Grossart, Helle Ploug, Niculina Musat
Summary: This study reveals the importance of bacteria colonization on sinking diatom aggregates for carbon and nitrogen cycling. The results suggest that different bacterial groups have varying levels of carbon and nitrogen uptake from diatoms, with Alteromonas group showing a competitive advantage in nitrogen-limited deep-sea environments.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Sarah Haenelt, Gangan Wang, Jonas Coelho Kasmanas, Florin Musat, Hans Hermann Richnow, Ulisses Nunes da Rocha, Jochen A. Mueller, Niculina Musat
Summary: Currently, there are few regulations on the discharge of antibiotics from wastewater treatment plants (WWTP), resulting in surface waters becoming a potential reservoir for antibiotics and antibiotic resistance genes (ARGs). In this study, the abundance of indicator genes and ARGs were monitored in a river system from its pristine source to WWTP-impacted water. The results showed that the WWTP effluent is the main source of sulfonamides and indicator genes, but downstream water quality improved with lower sulfonamide concentrations and decreasing abundances of sul1 and sul2.
FRONTIERS IN MICROBIOLOGY
(2023)