期刊
ISME JOURNAL
卷 6, 期 10, 页码 1869-1882出版社
SPRINGERNATURE
DOI: 10.1038/ismej.2012.24
关键词
metagenome; metatranscriptome; Chlorobi
资金
- NASA [NX09AM87G]
- US Department of Energy (DOE), Office of Biological and Environmental Research (BER)
- National Science Foundation [MCB-0523100, EF-0328698]
- Department of Energy [DE-FG02-94ER20137]
- DOE-Joint Genome Institute
- Danish Natural Science Research Council
- IGERT [DGE 0654336]
- Office of Science of the US Department of Energy [DE-AC02-05CH11231]
An uncultured member of the phylum Chlorobi, provisionally named 'Candidatus Thermochlorobacter aerophilum', occurs in the microbial mats of alkaline siliceous hot springs at the Yellowstone National Park. 'Ca. T. aerophilum' was investigated through metagenomic and metatranscriptomic approaches. 'Ca. T. aerophilum' is a member of a novel, family-level lineage of Chlorobi, a chlorophototroph that synthesizes type-1 reaction centers and chlorosomes similar to cultivated relatives among the green sulfur bacteria, but is otherwise very different physiologically. 'Ca. T. aerophilum' is proposed to be an aerobic photoheterotroph that cannot oxidize sulfur compounds, cannot fix N-2, and does not fix CO2 autotrophically. Metagenomic analyses suggest that 'Ca. T. aerophilum' depends on other mat organisms for fixed carbon and nitrogen, several amino acids, and other important nutrients. The failure to detect bchU suggests that 'Ca. T. aerophilum' synthesizes bacteriochlorophyll (BChl) d, and thus it occupies a different ecological niche than other chlorosome-containing chlorophototrophs in the mat. Transcription profiling throughout a diel cycle revealed distinctive gene expression patterns. Although 'Ca. T. aerophilum' probably photoassimilates organic carbon sources and synthesizes most of its cell materials during the day, it mainly transcribes genes for BChl synthesis during late afternoon and early morning, and it synthesizes and assembles its photosynthetic apparatus during the night. The ISME Journal (2012) 6, 1869-1882; doi:10.1038/ismej.2012.24; published online 29 March 2012
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