Article
Plant Sciences
Raj Kumar Verma, Doron Teper
Summary: In this study, researchers identified a novel avirulence protein called ChpG that is recognized in resistant eggplant varieties and restricts the host range of Clavibacter michiganensis.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
In Woong Park, In Sun Hwang, Eom-Ji Oh, Choon-Tak Kwon, Chang-Sik Oh
Summary: Clavibacter michiganensis causes bacterial canker and wilting in host plants. Nicotiana benthamiana is a surrogate host for C. michiganensis and C. capsici. C. michiganensis induced blister-like lesions, canker, and wilting in N. benthamiana, and the celA and pat-1 genes were not necessary for symptom development.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Alice Godson, Renier A. L. van der Hoorn
Summary: Apoplastic proteases play crucial roles in plant defense, with most being induced upon infection and 17 genetically required for the immune response. While the proteolytic activity of these proteases has been confirmed, its necessity for biological function is not always clear.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
In Sun Hwang, Eom-Ji Oh, Eunbee Song, In Woong Park, Yoonyoung Lee, Kee Hoon Sohn, Doil Choi, Chang-Sik Oh
Summary: Clavibacter michiganensis is a plant-pathogenic bacterium that causes disease through the secretion of effector proteins. The study reveals the role of Pat-1(Cm), a secreted protein carrying a functional catalytic triad, in both pathogenicity and plant immunity. Pat-1(Cm) plays a critical role in disease development and the hypersensitive response (HR) in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Xiaoliang Wang, Damjan Avsec, Ales Obreza, Shida Yousefi, Irena Mlinaric-Rascan, Hans-Uwe Simon
Summary: This study demonstrates that serine protease inhibitors can block necrosis triggered by CD44, preventing cell death in GM-CSF-primed neutrophils. The inhibitors inhibit the activation of MLKL, p38 MAPK, and PI3K. However, they have no effect on apoptosis mediated by the FAS receptor.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Shuaishuai Wang, Rongkang Xing, Yan Wang, Haidong Shu, Shenggui Fu, Jie Huang, Judith K. Paulus, Mariana Schuster, Diane G. O. Saunders, Joe Win, Vivianne Vleeshouwers, Yuanchao Wang, Xiaobo Zheng, Renier A. L. van der Hoorn, Suomeng Dong
Summary: The study reveals the important function of plant apoplastic proteases in cleaving pathogen proteins to release immunogenic peptides. A newly identified protein PC2 induces immunity in plants only after being cleaved by apoplastic proteases, and a core peptide of PC2 carrying key cysteines is found to be sufficient for inducing cell death. Additionally, protease inhibitors produced by the pathogen dampen the host immunity elicited by PC2.
Article
Plant Sciences
Zongxiang Zhan, Huishan Liu, Yao Yang, Shuang Liu, Xiaonan Li, Zhongyun Piao
Summary: In this study, 518 secretory proteins were screened and 63 candidate effectors were identified, two of which could induce cell death and were associated with H2O2 accumulation. Additionally, 55 candidate effectors were found to suppress BAX-induced cell death, and 21 of them could suppress the immunity caused by a bacterial pathogen.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Alejandro A. Puchol Tarazona, Daniel Maresch, Annette Grill, Janet Bakalarz, Juan A. Torres Acosta, Alexandra Castilho, Herta Steinkellner, Lukas Mach
Summary: Nicotiana benthamiana is a versatile host for manufacturing protein therapeutics, but the fidelity of recombinant proteins is compromised by proteolysis. Two subtilisin-like serine proteases (NbSBT1 and NbSBT2) have been identified as the main causes of proteolysis, suggesting that downregulating their activities could optimize the production of biopharmaceuticals on this platform. Sequence data of NbSBT are available in the DDBJ/EMBL/GenBank databases.
Article
Plant Sciences
Kamrun Nahar, Takafumi Mukaihara, Fumiko Taguchi, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Tomonori Shiraishi, Yuki Ichinose
Summary: HopH1 is an effector protein that induces HR cell death in a nonhost plant, and can confer the ability to induce HR in other bacteria when introduced.
JOURNAL OF GENERAL PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Man Zhou, You Lu, Gerit Bethke, Brian T. Harrison, Noriyuki Hatsugai, Fumiaki Katagiri, Jane Glazebrook
Article
Plant Sciences
Noriyuki Hatsugai, Aya Nakatsuji, Osamu Unten, Kimi Ogasawara, Maki Kondo, Mikio Nishimura, Tomoo Shimada, Fumiaki Katagiri, Ikuko Hara-Nishimura
Article
Plant Sciences
You Lu, William Truman, Xiaotong Liu, Gerit Bethke, Man Zhou, Chad L. Myers, Fumiaki Katagiri, Jane Glazebrook
Article
Genetics & Heredity
Li Lei, Ana M. Poets, Chaochih Liu, Skylar R. Wyant, Paul J. Hoffman, Corey K. Carter, Brian G. Shaw, Xin Li, Gary J. Muehlbauer, Fumiaki Katagiri, Peter L. Morrell
G3-GENES GENOMES GENETICS
(2019)
Article
Biochemical Research Methods
S. Ha, E. Dimitrova, S. Hoops, D. Altarawy, M. Ansariola, D. Deb, J. Glazebrook, R. Hillmer, H. Shahin, F. Katagiri, J. McDowell, M. Megraw, J. Setubal, B. M. Tyler, R. Laubenbacher
BMC BIOINFORMATICS
(2019)
Correction
Biochemistry & Molecular Biology
You Lu, Jianxin Xue, Tao Deng, Xiaojuan Zhou, Kun Yu, Lei Deng, Meijuan Huang, Xin Yi, Maozhi Liang, Yu Wang, Haige Shen, Ruizhan Tong, Wenbo Wang, Li Li, Jin Song, Jing Li, Xiaoxing Su, Zhenyu Ding, Youling Gong, Jiang Zhu, Yongsheng Wang, Bingwen Zou, Yan Zhang, Yanying Li, Lin Zhou, Yongmei Liu, Min Yu, Yuqi Wang, Xuanwei Zhang, Limei Yin, Xuefeng Xia, Yong Zeng, Qiao Zhou, Binwu Ying, Chong Chen, Yuquan Wei, Weimin Li, Tony Mok
Review
Biochemistry & Molecular Biology
You Lu, Kenichi Tsuda
Summary: The review discusses the recognition mechanisms of microbes by plants through PRRs and NLRs, as well as the crosstalk between the signals mediated by these receptors. It also proposes hypotheses for guiding further research on key topics in this field.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Endocrinology & Metabolism
Xiaoxue Qiu, Marissa G. Macchietto, Xiaotong Liu, You Lu, Yiwei Ma, Hong Guo, Milena Saqui-Salces, David A. Bernlohr, Chi Chen, Steven Shen, Xiaoli Chen
Summary: In this study, it was found that short-term high fat diet feeding stimulates intestinal Lcn2 expression, but as the feeding period prolongs, fecal Lcn2 levels decrease. Lcn2 deficiency accelerates the development of HFD-induced intestinal inflammation and microbiota dysbiosis, as well as leads to a remodeling of microbiota-derived metabolome.
INTERNATIONAL JOURNAL OF OBESITY
(2021)
Editorial Material
Plant Sciences
Zhengnan Jia, Miaomiao Ding, Masahito Nakano, Kunqi Hong, Ruidong Huang, Dieter Becker, Jane Glazebrook, Fumiaki Katagiri, Xiaowei Han, Kenichi Tsuda
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Qi Zheng, Kristina Majsec, Fumiaki Katagiri
Summary: The study investigated the evolutionary mechanisms among the CBP60a, CBP60g, and SARD1 immune regulatory subfamilies, revealing significant coevolutionary interactions across diverse core eudicot species lineages, consistent with hypothesized resilience-confering coevolution mechanisms to fight against fast-evolving pathogen effectors.
Article
Cell Biology
Yewei Ji, Yuan Luo, Yating Wu, Yao Sun, Lianfeng Zhao, Zhen Xue, Mengqi Sun, Xiaoqiong Wei, Zinan He, Shuangcheng Alivia Wu, Liangguang Leo Lin, You Lu, Lei Chang, Fei Chen, Siyu Chen, Wei Qian, Xiaoxi Xu, Shengnuo Chen, Dongli Pan, Zhangsen Zhou, Sheng Xia, Chih-Chi Andrew Hu, Tingbo Liang, Ling Qi
Summary: The SEL1L-HRD1 complex regulates STING protein levels in the endoplasmic reticulum and negatively regulates its activation. SEL1L-HRD1 deficiency amplifies STING signaling in macrophages and enhances immunity against viral infection and tumor growth. This study identifies a key role of SEL1L-HRD1 ERAD in innate immunity and provides a therapeutic approach for targeting STING.
NATURE CELL BIOLOGY
(2023)
Article
Plant Sciences
Fumiaki Katagiri
Summary: Flowering plants have developed a highly resilient core immunity that has allowed them to withstand attacks from fast-evolving pathogens for millions of years. One key mechanism for this resilience is the acquisition of backup immune signaling mechanisms that conceal evolutionary goals from pathogens. However, it is unlikely that these backup mechanisms evolved through incremental adaptation under constant pressure from faster-evolving organisms. This paper proposes a hypothesis for the evolution of a system with backup mechanisms, drawing inspiration from the multiverse hypothesis in cosmology.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Multidisciplinary Sciences
Shuangcheng Alivia Wu, Chenchen Shen, Xiaoqiong Wei, Xiawei Zhang, Siwen Wang, Xinxin Chen, Mauricio Torres, You Lu, Liangguang Leo Lin, Huilun Helen Wang, Allen H. Hunter, Deyu Fang, Shengyi Sun, Magdalena I. Ivanova, Yi Lin, Ling Qi
Summary: Endoplasmic reticulum-associated degradation (ERAD) and ER-phagy are two central degradative mechanisms in the ER. This study describes the sequence of events underlying the disposition of misfolded ER proteins by ERAD and ER-phagy.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Benedict Abdon, Yusheng Liang, Debora da Luz Scheffer, Mauricio Torres, Neha Shrestha, Rachel B. Reinert, You Lu, Brent Pederson, Amara Bugarin-Lapuz, Sander Kersten, Ling Qi
Summary: The SEL1L-HRD1 ERAD complex is essential for maintaining postnatal muscle growth and systemic energy balance. Deletion of SEL1L in myocytes impairs muscle hypertrophy and leads to a reduced overall body growth. This deletion also triggers metabolic reprogramming characterized by improved glucose sensitivity, enhanced beigeing of adipocytes, and resistance to diet-induced obesity.
Article
Medicine, Research & Experimental
Neha Shrestha, Mauricio Torres, Jason Zhang, You Lu, Leena Haataja, Rachel B. Reinert, Jeffrey Knupp, Yu-Jie Chen, Gunes Parlakgul, Ana Paula Arruda, Billy Tsai, Peter Arvan, Ling Qi
Summary: This study reveals the intricate crosstalk among three ER quality-control mechanisms, namely, the unfolded protein response, ER-associated degradation, and ER-phagy, in the dynamic regulation of organellar architecture and pancreatic β-cell function. The SEL1L-HRD1 protein complex of ERAD plays a central role in this process. Disruption of SEL1L-HRD1 ERAD leads to activation of autophagy and retention of proinsulin in the ER, which is subsequently cleared via ER-phagy. Loss of both SEL1L and autophagy in pancreatic β-cells results in diabetes and premature death in mice. Focused ion beam scanning electron microscopy and deep-learning automated image segmentation reveal a profound organellar restructuring with a massive expansion of ER volume and network in cells lacking both SEL1L and autophagy.
JOURNAL OF CLINICAL INVESTIGATION
(2023)