Article
Biochemistry & Molecular Biology
Muhammad Babar Khan, Rosamaria Ruggieri, Eesha Jamil, Nhan L. Tran, Camila Gonzalez, Nancy Mugridge, Steven Gao, Jennifer MacDiarmid, Himanshu Brahmbhatt, Jann N. Sarkaria, John Boockvar, Marc Symons
Summary: The study suggests that microRNA-34a can effectively reduce the activation status of glioblastoma cells and enhance survival rates against multiple glioblastoma cell types. Combination therapy of temozolomide with microRNA-34a loaded nanocells targeted to epidermal growth factor receptor (EGFR) significantly enhances sensitivity of glioblastoma cells to treatment.
MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yi-Yung Hung, Chen-Kai Chou, Yi-Chien Yang, Hung-Chun Fu, El-Wui Loh, Hong-Yo Kang
Summary: This study found that in patients with major depressive disorder, the expression levels of miR-146a in exosomes were higher, and patients who achieved remission showed lower levels of various microRNAs before treatment and increased levels after treatment. Let-7e, miR-145, and miR-146a showed acceptable discrimination between the remission and non-remission groups.
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Marta Correia de Sousa, Nicolas Calo, Cyril Sobolewski, Monika Gjorgjieva, Sophie Clement, Christine Maeder, Dobrochna Dolicka, Margot Fournier, Laurent Vinet, Xavier Montet, Jean-Francois Dufour, Bostjan Humar, Francesco Negro, Christine Sempoux, Michelangelo Foti
Summary: Despite being considered a potent oncomiR in human cancers, genetic deletion of miR-21 in mouse models surprisingly promotes hepatocellular carcinoma (HCC) development by affecting various signaling pathways and immune responses. These results challenge the current belief in miR-21 as an oncogene and raise caution regarding using miR-21 inhibition as a therapeutic approach for HCC.
Article
Engineering, Biomedical
Yuanyuan Liu, Meng Zheng, Mingzhu Jiao, Chengnan Yan, Sen Xu, Qiuli Du, Marco Morsch, Jinlong Yin, Bingyang Shi
Summary: This study developed a targeted polymeric nanoparticle combining polymeric nanotechnology with miRNA regulation technology to effectively treat GBM. The nanoparticle can protect miRNA from enzyme degradation in the blood and enable targeted delivery to GBM tissue. Co-delivery of anti-miR-21 and miR-124 can regulate tumor cell signaling pathways, achieving combinatorial GBM therapy.
Review
Cell Biology
Romain Larrue, Sandy Fellah, Cynthia van der Hauwaert, Marie-Flore Hennino, Michael Perrais, Arnaud Lionet, Francois Glowacki, Nicolas Pottier, Christelle Cauffiez
Summary: MicroRNAs (miRNAs) are small RNA species that control gene expression and play important roles in kidney development, homeostasis, and disease treatment. miR-21, a well-studied miRNA, has attracted much attention in renal physiology due to its conservation and pathogenic role in various renal diseases. This review summarizes the involvement of miR-21 in renal homeostasis and diseases, particularly its dual role in acute and chronic kidney injuries, and discusses its potential as a biomarker and therapeutic target in renal diseases.
Article
Cell Biology
Fabiola Olivieri, Francesco Prattichizzo, Angelica Giuliani, Giulia Matacchione, Maria Rita Rippo, Jacopo Sabbatinelli, Massimiliano Bonafe
Summary: Inflammaging is a key concept in understanding how aging contributes to the development of age-related diseases, with a focus on the role of miRNAs in regulating inflammatory pathways. The assessment of individual genetic make-up alone is insufficient to depict the trajectories of age-related inflammation, and studies have shown that circulating inflammamiRs could be used to measure the degree of inflammaging.
AGEING RESEARCH REVIEWS
(2021)
Review
Oncology
Ana E. Jenike, Marc K. Halushka
Summary: miRNA-21 is considered as a specific predictive or prognostic biomarker for at least 29 diseases, but lacks specificity for any one disease, thus making it not an ideal candidate for a biomarker despite continued evaluation. This issue of non-specificity in biomarker designation is shared with other common, ubiquitous miRNAs and warrants concern for their use as biomarkers as well.
BIOMARKER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Hao Jia, Wei Sun, Xiangbo Li, Wenhao Xu
Summary: MEL has been found to effectively inhibit the growth of thyroid cancer and suppress tumor cell proliferation while promoting apoptosis. MEL also regulates the expression of certain genes and proteins to further promote tumor cell apoptosis.
Article
Biochemistry & Molecular Biology
Liana Maries, Alexandra Ioana Moatar, Maria Sala-Cirtog, Laurentiu Sima, Andrei Anghel, Catalin Marian, Aimee Rodica Chis, Ioan-Ovidiu Sirbu
Summary: Left ventricle remodeling after acute myocardial infarction is a significant contributor to heart failure, but predicting it remains challenging. This study found that certain circulant microRNAs may not be effective in distinguishing patients with left ventricle remodeling from those without.
Article
Nanoscience & Nanotechnology
Zhongshan Wang, Guangsheng Wu, Zhujun Yang, Xuejian Li, Zhihong Feng, Yimin Zhao
Summary: In this study, miR-21-loaded chitosan/tripolyphosphate/hyaluronic acid nanoparticles were prepared and formed a stable biological seal on titanium surfaces. The miR-21-functionalized titanium surface enhanced fibroblast adhesion, proliferation, and expression of extracellular matrix-related genes.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Physical
Cheng-Hu Hu, Bing-Dong Sui, Jin Liu, Lei Dang, Ji Chen, Chen-Xi Zheng, Songtao Shi, Na Zhao, Min-Yan Dang, Xiao-Ning He, Li-Qiang Zhang, Ping-Ping Gao, Nan Chen, Hui-Juan Kuang, Kai Chen, Xiao-Lin Xu, Xiao-Rui Yu, Ge Zhang, Yan Jin
Summary: Sympathetic neurostimulation through beta(1/2)-adrenergic receptor signaling triggers the transcription of miR-21 in osteoblasts, which is then transferred to osteoclast progenitors via exosomes to dictate osteoclastogenesis. Pharmacological inhibition of exosome release can suppress this miR-21 transfer and ameliorate bone loss under stress-induced depression conditions.
Article
Chemistry, Analytical
Tao Dong, Ran Yin, Qingcai Yu, Wanwei Qiu, Kun Li, Lisheng Qian, He Li, Bing Shen, Guodong Liu
Summary: The study presents a highly sensitive approach for detecting microRNA-21 (miR-21) in cancer cells and human serum using Au@Si nanocomposite labeled lateral flow assay. After systematic optimization, the method can detect a minimum concentration of 1.0 pM miR-21, which is 60 times lower than that of the GNP-based lateral flow assay.
ANALYTICA CHIMICA ACTA
(2021)
Review
Biochemistry & Molecular Biology
Amir Mohammad Malvandi, Seyed Hamidreza Rastegar-Moghaddam, Saeede Ebrahimzadeh-Bideskan, Giovanni Lombardi, Alireza Ebrahimzadeh-Bideskan, Abbas Mohammadipour
Summary: Spinal cord injury (SCI) is a devastating neurological condition that causes physical disability, psychological stress, and financial burden. miR-21, which is highly expressed in SCI, has been found to have protective effects against SCI by inhibiting inflammation, improving blood-spinal cord barrier function, regulating angiogenesis, and controlling glial scar formation. This review provides a new therapeutic perspective for miR-21 in SCI.
MOLECULAR MEDICINE
(2022)
Article
Medicine, General & Internal
Pawel Rubis, Justyna Toton-Zuranska, Maria Kolton-Wroz, Pawel Wolkow, Ewelina Pitera, Sylwia Wisniowska-Smialek, Ewa Dziewiecka, Ann Garlitski, Piotr Podolec
Summary: This study investigated the kinetics of fibrosis-linked microRNAs and their relationship with extracellular matrix (ECM) fibrosis in dilated cardiomyopathy (DCM). The results showed that regardless of ECM fibrosis status or duration of the disease, the 12-month patterns of circulating microRNAs were similar in DCM patients. Additionally, the correlations between microRNAs measured at 3 and 12 months were lower than expected, and circulating microRNAs were not able to differentiate between DCM patients with and without fibrosis.
ARCHIVES OF MEDICAL SCIENCE
(2022)
Review
Anatomy & Morphology
Siddhant Suri Dhawan, Vivek Yedavalli, Tarik F. Massoud
Summary: Organisms may retain nonfunctional anatomical features as a consequence of evolutionary natural selection. Resultant atavistic and vestigial anatomical structures, which reappear after loss or are remnants with reduced or no function, can be puzzling and often overlooked. This article provides an overview of important atavistic and vestigial structures in the head, neck, and spine and their clinical significance. Understanding these structures can inform diagnoses, prevent misinterpretation, and guide clinical interventions.
ANATOMICAL SCIENCE INTERNATIONAL
(2023)
Article
Acoustics
Yanrong Zhang, Jing Wang, Sara Natasha Ghobadi, Haiyan Zhou, Ai Huang, Marco Gerosa, Qingyi Hou, Olivier Keunen, Anna Golebiewska, Frezghi G. Habte, Gerald A. Grant, Ramasamy Paulmurugan, Kevin S. Lee, Max Wintermark
Summary: Transient, focal opening of the blood-brain barrier (BBB) using magnetic resonance imaging (MRI)-guided focused ultrasound (MRgFUS) combined with microbubbles can alter the composition of tumor-associated macrophages and microglia (TAMs) and induce a shift towards a more pro-inflammatory status of the immune environment in glioblastoma.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Seong Yeol Kim, Ramar Thangam, Nayeon Kang, Hyunsik Hong, Chowon Kim, Sungkyu Lee, Subin Son, Hyun-Jeong Lee, Kyong-Ryol Tag, Sunhong Min, Daun Jeong, Jangsun Hwang, Kanghyeon Kim, Dahee Kim, Yuri Kim, Jinmyoung Joo, Bong Hoon Kim, Yangzhi Zhu, Sung-Gyu Park, Hyun-Cheol Song, Wujin Sun, Jae-Pyoung Ahn, Woo Young Jang, Ramasamy Paulmurugan, Hong-Kyu Kim, Jong Seung Kim, Heemin Kang
Summary: In this study, the linking molecule-mediated conjugation of 1D magnetic nanocylinders (MNCs) to material surfaces pre-decorated with gold nanospheres (GNSs) was performed to establish a non-bridging state for RGD-coated MNCs (RGD-MNCs) over RGD-coated GNSs (RGD-GNSs). The use of magnetic field-mediated compression of the linking molecules enabled bridging between RGD-MNCs and RGD-GNSs. The optimized proportion of RGD-MNCs to RGD-GNSs allowed for effective remote stimulation of integrin binding and modulation of macrophages for immunomodulatory responses.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Editorial Material
Medicine, General & Internal
Jessica T. Wen, Tarik F. Massoud
AMERICAN JOURNAL OF MEDICINE
(2023)
Article
Anatomy & Morphology
Brian Dang, Fabian N. Necker, Siddhant Suri Dhawan, Tara Murty, Tarik F. Massoud
Summary: This study retrospectively analyzed MRI images of 34 healthy individuals to reveal the structure and dimensions of the caudolenticular gray bridges (CLGBs) in normal individuals. These findings will guide future studies on the potential role of CLGBs morphometry in predisposition to Parkinson's disease.
Article
Hematology
Sumanth Mosale Seetharam, Yi Liu, Jason Wu, Lenn Fechter, Kanagavel Murugesan, Holden Maecker, Jason Gotlib, James Zehnder, Ramasamy Paulmurugan, Anandi Krishnan
Summary: Impaired protein homeostasis plays a significant role in myeloproliferative neoplasms (MPNs). This study identifies the downregulation of enkurin (ENKUR) and the upregulation of CDC20 as potential markers of MPN pathogenesis, suggesting a novel role for ENKUR in MPNs.
Correction
Nanoscience & Nanotechnology
Uday K. K. Sukumar, Jagathesh Chandra Bose Rajendran, Sanjiv S. S. Gambhir, Tarik F. F. Massoud, Ramasamy Paulmurugan
ACS APPLIED MATERIALS & INTERFACES
(2023)
Correction
Chemistry, Multidisciplinary
RajendranJ C. Bose, Sukumar Uday Kumar, Yitian Zeng, Rayhaneh Afjei, Elise Robinson, Kenneth Lau, Abel Bermudez, Frezghi Habte, Sharon J. Pitteri, Robert Sinclair, Juergen K. Willmann, Tarik F. Massoud, Sanjiv S. Gambhir, Ramasamy Paulmurugan
Correction
Chemistry, Multidisciplinary
Uday S. S. Kumar, Rayhaneh Afjei, Katherine Ferrara, Tarik F. F. Massoud, Ramasamy Paulmurugan
Proceedings Paper
Acoustics
Negar Sadeghipour, Farbod Tabesh, Arutselvan Natarajan, Meredith A. Jones, Xuxin Chen, Ramasamy Paulmurugan, Bin Zheng, Ahmed El Kaffas
Summary: Molecular ultrasound imaging utilizes microbubbles that are conjugated with specific proteins to image their expression on blood vessels. However, optimal application of microbubbles in ultrasound imaging system for detecting and quantifying protein expression requires further investigation.
MEDICAL IMAGING 2023
(2023)
Article
Biochemical Research Methods
Nisi Zhang, Yutong Guo, Josquin Foiret, Spencer K. Tumbale, Ramasamy Paulmurugan, Katherine W. Ferrara
Summary: This study presents a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. The protocol includes steps of computational simulation, experimental calibration, reagent preparation, ultrasound treatment, validation, and gene expression analysis. It utilizes approved diagnostic microbubbles and parameters applicable for human use.
Article
Biochemistry & Molecular Biology
Sung-Bae Kim, Ramasamy Paulmurugan, Nobuo Kitada, Sojiro A. Maki
Summary: Single-chain multicolor-reporter imaging templates were developed for the subcellular localization of molecular events in mammalian cells. These templates consist of fluorescent protein variants fused with luciferases and subcellular localization signal peptides, allowing simultaneous reporting of steroid hormonal activities in subcellular compartments at different optical spectra. The templates contribute to the expansion of a toolbox of optical probes for subcellular localization of molecular events in intact cells.
RSC CHEMICAL BIOLOGY
(2023)