期刊
MOLECULAR PHARMACEUTICS
卷 13, 期 1, 页码 163-171出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.molpharmaceut.5b00594
关键词
BSEP; classification model; inhibition; drug-induced cholestasis; bromocriptin
资金
- Innovative Medicines Initiative Joint Undertaking from European Union [115002]
- Austrian Science Fund (FWF) [F03502, F03509, F03520]
- SNF National Center of Competence in Research TransCure (University of Berne, Berne, Switzerland)
The bile salt export pump (BSEP) is an ABC-transporter expressed at the canalicular membrane of hepatocytes. Its physiological role is to expel bile salts into the canaliculi from where they drain into the bile duct. Inhibition of this transporter may lead to intrahepatic cholestasis. Predictive computational models of BSEP inhibition may allow for fast identification of potentially harmful compounds in large databases. This article presents a predictive in silico model based on physicochemical descriptors that is able to flag compounds as potential BSEP inhibitors. This model was built using a training set of 670 compounds with available BSEP inhibition potencies. It successfully predicted BSEP inhibition for two independent test sets and was in a further step used for a virtual screening experiment. After in vitro testing of selected candidates, a marketed drug, bromocriptin, was identified for the first time as BSEP inhibitor. This demonstrates the usefulness of the model to identify new BSEP inhibitors and therefore potential cholestasis perpetrators.
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