Article
Biochemistry & Molecular Biology
Li Xiao, Mai Mochizuki, Dongliang Wang, Naohiro Shimamura, Katsuhisa Sunada, Taka Nakahara
Summary: This study developed an environment-friendly cell culture insert, XL-insert, which can reduce plastic waste with lower cost. Compared to two types of commercial disposable culture inserts (Col-inserts and PET-inserts), XL-inserts showed better results in cell-cell interactions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Fabrizio Fontana, Martina Anselmi, Patrizia Limonta
Summary: It has been found that obesity is associated with a higher risk of prostate cancer. The interaction between adipose tissue and prostate cancer is not fully understood. This study showed that adipocyte conditioned media can induce stemness properties in prostate cancer cells and promote certain gene expressions. Furthermore, exposure to adipocyte conditioned media led to partial epithelial-to-mesenchymal transition in the cancer cells, resulting in enhanced tumor aggressiveness and chemoresistance.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2023)
Article
Endocrinology & Metabolism
Abir Mukherjee, Divya Bezwada, Francesco Greco, Malu Zandbergen, Tong Shen, Chun-Yi Chiang, Medine Tasdemir, Johannes Fahrmann, Dmitry Grapov, Michael R. La Frano, Hieu S. Vu, Brandon Faubert, John W. Newman, Liam A. Mcdonnell, Luigi Nezi, Oliver Fiehn, Ralph J. Deberardinis, Ernst Lengyel
Summary: This study reveals that adipocytes in the tumor microenvironment contribute to macromolecular biosynthesis in cancer cells by providing an alternate fuel source. Through multiple experiments, it is found that glucose-derived carbons are utilized for the biosynthesis of glycerol-3-phosphate, resulting in increased lipid synthesis and promoting metastasis. Targeting biosynthetic processes specific to adipose-rich tumor microenvironments might be an effective strategy against metastasis.
Article
Biochemistry & Molecular Biology
Gayathri Visakan, Jingtan Su, Janet Moradian-Oldak
Summary: Studies on animal models have shown that ameloblastin (AMBN) is involved in cell-matrix adhesion and cell polarization during enamel formation. In this study, an in vitro 3D cell culture model was developed to investigate the role of AMBN in cell polarization and morphology. The addition of AMBN to the culture matrix resulted in the clustering and elongation of ameloblast cell lines in a dose-dependent manner. AMBN plays a critical signaling role in the initiation of cell polarity by acting as a communicator between cell-cell and cell-matrix interactions.
Article
Acoustics
D. Cody Morris, Derek Y. Chan, Mark L. Palmeri, Thomas J. Polascik, Wen-Chi Foo, Kathryn R. Nightingale
Summary: SWEI is more sensitive and specific for prostate cancer biopsy guidance compared to TRUS B-mode imaging, capable of distinguishing PCa from healthy tissue based on shear wave speed, and yields higher specificity and positive predictive value after normalizing for tissue compression with shear wave speed ratio consideration.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Article
Endocrinology & Metabolism
R. Aird, J. Wills, K. F. Roby, C. Benezech, R. H. Stimson, M. Wabitsch, J. W. Pollard, A. Finch, Z. Michailidou
Summary: This study found that hypoxia in the adipose-tumour microenvironment promotes lipid uptake in cancer cells, leading to increased proliferation. Additionally, hypoxia alters the metabolome of adipocytes and drives proliferation of non-malignant cells.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Oncology
Xu-ke Qin, Yang Du, Xiu-heng Liu, Lei Wang
Summary: Prostate cancer is the most common cancer in men worldwide, and castration-resistant prostate cancer is incurable and prone to drug resistance. Up-regulated LSD1 in prostate cancer can destabilize cancer suppressor protein FBXW7 without its demethylation function, promoting cancer cell survival. Inhibition of LSD1-FBXW7 interaction by the allosteric inhibitor SP-2509 can suppress cancer cell survival, suggesting a potential new direction for prostate cancer treatment research.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Nina Compera, Scott Atwell, Johannes Wirth, Christine von Toerne, Stefanie M. Hauck, Matthias Meier
Summary: This study developed a microfluidic chip platform to simulate adipose tissue in vitro and revealed the differences in adipocytes under low/high glucose conditions using proteomics analysis. This in vitro model can be used to investigate the molecular and functional mechanisms of adipose tissue under normal and pathological conditions.
Article
Oncology
Elena D. Czyrnik, Marc Wiesehoefer, Jaroslaw T. Dankert, Sven Wach, Mathias Wagner, Martin Spahn, Marianna Kruithof de Julio, Gunther Wennemuth
Summary: This study reveals the mechanism of cell-cell interaction via soluble factors in prostate carcinoma and highlights the importance of GALNT14.
FRONTIERS IN ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ajay Dhakal, Luna Acharya, Ruth O'Regan, Shipra Gandhi, Carla Falkson
Summary: The derangement of the PI3K pathway is closely related to various subtypes of breast cancer, making it a promising molecular target for systemic therapy. While several drugs targeting PI3K have been or are being investigated in clinical trials, the search for an effective and safe PI3K inhibitor for breast cancer treatment continues. This review focuses on completed and ongoing clinical trials of PI3K inhibitors in breast cancer, highlighting their efficacy and potential challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eriko Suzuki, Naoki Masaka, Tatsuhiro Urabe, Madoka Sasaki, Keiji Hasumi
Summary: This study reveals that the direct interaction between cancer stem cells (CSCs) and non-CSCs inhibits the division of CSCs, providing insights into the regulatory mechanism of non-CSCs on CSC proliferation in the tumor microenvironment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Yongliang Fan, Long Jin, Zhiping He, Tiantian Wei, Tingting Luo, Jiaman Zhang, Can Liu, Changjiu Dai, A. Chao, Yan Liang, Xuan Tao, Xuebin Lv, Yiren Gu, Mingzhou Li
Summary: Studying the composition and developmental mechanisms in mammary gland is important for newborn health. The transcriptomic dataset of porcine mammary gland cells revealed distinct phenotypic and transcriptional signatures at different developmental stages, showing differentiation and proliferation processes of adipocytes and epithelial cells.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Peng Gu, Minhao Zhang, Xinhua Chen, Jie Du, Lu Chen, Xiaoliang He, Meilin Li
Summary: The study found that CDCA3 expression is increased in prostate cancer and high expression is correlated with poor prognosis. CDCA3 may serve as a diagnostic and prognostic marker for prostate cancer, as well as a potential therapeutic target.
Article
Oncology
Rongrong Jiang, Jiechun Huang, Xiaotian Sun, Xianglin Chu, Fangrui Wang, Jie Zhou, Qihui Fan, Liewen Pang
Summary: Cancer metastasis is the leading cause of death in cancer patients, and there is still a lack of drugs targeting metastasis. The current 2D culture methods and animal models are unable to fully meet the needs for studying cell invasion. Therefore, the development of a precise and efficient 3D in vitro model is crucial for cell invasion research and drug screening tests.
Article
Oncology
Dorine Bellanger, Clea Dziagwa, Cyrille Guimaraes, Michelle Pinault, Jean-Francois Dumas, Lucie Brisson
Summary: The study reveals that adipocytes promote autophagy in breast cancer cells through the acidification of lysosomes, leading to cancer cell survival in nutrient-deprived conditions and to cancer cell migration. The interaction between white adipose tissue and cancer cells through this mechanism may play a central role in cancer progression.