Article
Biochemistry & Molecular Biology
Marjolein E. Crooijmans, Tijn O. Delzenne, Tim Hensen, Mina Darehei, Johannes H. de Winde, G. Paul H. van Heusden
Summary: The expression of low or high affinity phosphate transporters in yeast cells is dependent on phosphate availability, with specific genes such as RRP6 and BMH1 playing a role in regulating the expression of the regulator Spl2. Deletion of certain genes can result in changes in expression levels of specific regulators, leading to variations in gene expression among cells.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Microbiology
Yoshihiro Ojima, Kyohei Naoi, Riho Akiyoshi, Masayuki Azuma
Summary: In this study, the amount and form of accumulated phosphate as well as its utilization in PHO mutant strains of Saccharomyces cerevisiae were quantitatively analyzed. It was found that the accumulated phosphate existed as polyphosphoric acid and served as a phosphorus source for the cells. This is the first report on the quantitative analysis of phosphate accumulation and utilization in PHO regulatory system-mutant strains of Saccharomyces cerevisiae.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
G. Paul H. van Heusden
Summary: Sense and antisense transcription levels have important effects on the expression of PHO genes and the regulation of SPL2 protein in yeast under phosphate starvation. Replacement of the transcriptional terminator of PHO84 resulted in increased antisense transcription, decreased sense transcription, and reduced expression of PHO84 and SPL2. The expression of unrelated genes was also affected. These results suggest that antisense transcription of PHO84, rather than the Pho84 transporter, regulates the expression of SPL2.
Article
Biochemistry & Molecular Biology
Deniz Irvali, Fabian P. Schlottmann, Prathibha Muralidhara, Iliya Nadelson, Katja Kleemann, N. Ezgi Wood, Andreas Doncic, Jennifer C. Ewald
Summary: In yeast cells, the decision at the Start point can be reversed, as cells that have passed Start can re-import the cell cycle inhibitor Whi5 into the nucleus and regain sensitivity to signaling molecules. This finding suggests that cell cycle commitment in yeast is a multi-step process.
Review
Plant Sciences
Shengnan Zhu, Cuiyue Liang, Jiang Tian, Yingbin Xue
Summary: This review summarizes recent discoveries and molecular basis of plant adaptive mechanisms to low phosphate availability, providing insights into the molecular mechanisms underlying plant adaptation to phosphorus starvation and facilitating the development of crop cultivars with high phosphorus utilization efficiency.
Review
Biochemistry & Molecular Biology
Francisco Gutierrez-Santiago, Francisco Navarro
Summary: Ribosomes are vital for protein production, and their biogenesis is regulated by cellular energy status and stress signals. In eukaryotic cells, stress signals and newly-synthesized ribosomes require transcription by RNA polymerases. The Target of Rapamycin pathway in eukaryotes influences RNA polymerase transcription to ensure proper ribosome production. This review focuses on how TOR regulates transcription in Saccharomyces cerevisiae and the coordination of three RNA polymerases.
Article
Endocrinology & Metabolism
Hyejin Lee, Eunmi Kim, Eun-Ae Shin, Jong Cheol Shon, Hyunseung Sun, Ji Eon Kim, Jae Woo Jung, Haesong Lee, Yangie Pinanga, Dae-Geun Song, Kwang-Hyeon Liu, Jung Weon Lee
Summary: This study investigated the modulation of hepatic fructose metabolism by TM4SF5 and found that it can regulate fructose uptake and lipogenesis by interacting with GLUT8, thereby affecting liver steatosis.
MOLECULAR METABOLISM
(2022)
Article
Multidisciplinary Sciences
Wenting Shen, Ziqing Gao, Kaiyue Chen, Alusi Zhao, Qi Ouyang, Chunxiong Luo
Summary: Cells respond to environmental changes by modulating gene expression. In this study, the responses to osmotic stress in glucose-limited and glucose-rich environments were compared. It was found that cells exhibited a stronger osmotic gene expression response and larger integral of Hog1 nuclear localization under glucose-limited conditions. The regulatory mechanism of osmoresponse was derived and a reserve flux-dependent stress-defense strategy was proposed.
Article
Chemistry, Multidisciplinary
Karolina Nowosad, Monika Sujka, Ewelina Zielinska, Urszula Pankiewicz
Summary: This study aims to optimize the PEF parameters to increase the accumulation of vitamin C in yeast cells, evaluate the effect of electroporation on biomass and yeast viability, and assess the effect of storage conditions on the vitamin C content and its antioxidant activity.
APPLIED SCIENCES-BASEL
(2022)
Article
Plant Sciences
Shichao Wang, Tingting Xu, Min Chen, Liyan Geng, Zhaoyang Huang, Xiaoli Dai, Hongye Qu, Jun Zhang, Huanhuan Li, Mian Gu, Guohua Xu
Summary: A WRKY transcription factor, OsWRKY10, acts as a negative regulator by suppressing the expression of OsPHT1;2 gene in rice, thereby inhibiting phosphate uptake from phosphate-replete environments.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Endocrinology & Metabolism
Wanping Chen, Xilan Yu, Yinsheng Wu, Jie Tang, Qi Yu, Xiaodong Lv, Zitong Zha, Bicheng Hu, Xin Li, Jianguo Chen, Lixin Ma, Jerry L. Workman, Shanshan Li
Summary: Through studying budding yeast and human endothelial cells, the researchers found that increased synthesis of acetyl-CoA in the nucleus can promote histone acetylation at subtelomeric regions, leading to telomere silencing defects and cellular senescence.
Article
Food Science & Technology
Pieter W. J. Labuschagne, Steohanie Rollero, Benoit Divol
Summary: The study found that B-complex vitamins significantly impact the fermentation performance of Kluyveromyces marxianus IWBT Y885, especially thiamine, which is crucial for ensuring optimal fermentation rate and sugar utilization. Higher concentrations of thiamine may lead to delayed uptake in K. marxianus Y885, and the different metabolites produced in wine fermentations between this yeast and others suggest that thiamine concentration affects the carbon metabolic flux differently in these yeasts, potentially influencing final wine properties.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kunio Nakatsukasa, Munetaka Fujisawa, Xiaotan Yang, Tomoyuki Kawarasaki, Fumihiko Okumura, Takumi Kamura
Summary: This study found that Tgl4 is a stable protein that can be inactivated through dephosphorylation, playing an important regulatory role in the cell cycle.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Thoma Itoh, Takashi Makino
Summary: Recent advancements in high throughput single cell RNA-seq have enabled the identification of functional compensation effects of gene perturbations through noise change, which can be measured quantitatively and captured in gene regulatory networks.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Bazile Ravoityte, Juliana Luksa, Ralf Erik Wellinger, Saulius Serva, Elena Serviene
Summary: This study analyzed the impact of L-A virus on gene expression in yeast hosts using high-throughput RNA sequencing data. It was found that the presence of L-A virus resulted in moderate alterations in gene expression, particularly in genes involved in ribosome biogenesis. Additionally, the transcriptional responses to L-A maintenance were similar to those induced by stress or nutrient availability. The study also identified yeast transcriptional regulators that modulate the levels of L-A dsRNA in cells.
Article
Biochemistry & Molecular Biology
Pradeep Kumar Yadav, Ram Rajasekharan
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Praveen Kumar Rajvanshi, Madhuri Arya, Ram Rajasekharan
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Genetics & Heredity
Monala Jayaprakash Rao, Malathi Srinivasan, Ram Rajasekharan
Article
Plant Sciences
Varadarajan Venkateshwari, Anitha Vijayakumar, Arun Kumar Vijayakumar, L. Prasanna Anjaneya Reddy, Malathi Srinivasan, Ram Rajasekharan
Article
Multidisciplinary Sciences
Iyappan Ramachandiran, Anitha Vijayakumar, Visvanathan Ramya, Ram Rajasekharan
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Anusha Jahagirdar, Dandamudi Usharani, Malathi Srinivasan, Ram Rajasekharan
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Biology
Arvind A. Thekkingha, Kamlesh K. Yadav, Pundi N. Rangarajan
Article
Cell Biology
Martijn R. Molenaar, Kamlesh K. Yadav, Alexandre Toulmay, Tsjerk A. Wassenaar, Muriel C. Mari, Lucie Caillon, Aymeric Chorlay, Ivan E. Lukmantara, Maya W. Haaker, Richard W. Wubbolts, Martin Houweling, Arie Bas Vaandrager, Xavier Prieur, Fulvio Reggiori, Vineet Choudhary, Hongyuan Yang, Roger Schneiter, Abdou Rachid Thiam, William A. Prinz, J. Bernd Helms
Summary: The study demonstrates an alternative pathway of lipid droplet biogenesis independent of seipin, where retinyl esters can spontaneously accumulate and form lipid droplets in lipid bilayers. Even in cells that do not normally produce retinyl esters, the production of retinyl esters can induce the formation of lipid droplets.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ravi Chidambaram, Gowsalya Ramachandran, Ram Rajasekharan, Vasanthi Nachiappan
Summary: This study elucidated the important role of the transcription factor GCR1 in lipid metabolism in Saccharomyces cerevisiae. The study confirmed the binding of GCR1 to the OPI3 promoter region and its regulation of OPI3 expression. The study also demonstrated the impact of GCR1 on lipid homeostasis, including the level of phosphatidylcholine and the number and size of lipid droplets.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Review
Genetics & Heredity
Pradeep Kumar Yadav, Ram Rajasekharan
Review
Genetics & Heredity
Pradeep Kumar Yadav, Praveen Kumar Rajvanshi, Ram Rajasekharan
Review
Genetics & Heredity
Neelima Singh, Kamlesh Kumar Yadav, Ram Rajasekharan
Article
Biochemistry & Molecular Biology
Madhuri Arya, Malathi Srinivasan, Ram Rajasekharan
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)