Article
Chemistry, Multidisciplinary
Jay Min Lim, Rahul Tevatia, Ravi F. Saraf
Summary: This study found that small nucleic acids have a significant impact on the limit of quantification in qPCR analysis, primarily due to the length of the PCR product. Therefore, it is crucial to consider the size of the nucleic acids and the length of the PCR product during primer design for qPCR.
Article
Entomology
Yue Cao, Baishu Li, Naizhong Chen, Ding Yang, Li Li, Tao Liu
Summary: In this study, seven internal reference genes of Bactrocera dorsalis under various quarantine treatments were screened, with G6PDH and RPL-32 identified as the most stable genes for analyzing phytosanitary treatment in B. dorsalis. This research provides a foundation for further understanding the resistance mechanisms of B. dorsalis to abiotic stresses.
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Yaping Liu, Wanting Zhong, Shuo Feng, Ziteng Tang, Yingzhi Zhang, Lianzhong Ai, Zhiqiang Xiong
Summary: In this study, gene expression variance in B. animalis was analyzed using RNA sequencing, and stable reference genes were identified. Through integration of multiple analysis algorithms, rplD and atpA were determined as stable reference genes. Using these two genes for colony counting, accurate results were obtained in fermented skim milk. This study provides a foundation for accurate analysis of colony counting of B. animalis in dairy foods.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Public, Environmental & Occupational Health
E. Murillo-Zamora, O. Mendoza-Cano, I Delgado-Enciso, C. M. Hernandez-Suarez
Summary: The study identified factors predicting severe symptomatic laboratory-confirmed SARS-CoV-2 reinfection, including increasing age, comorbidities (such as obesity, asthma, type 2 diabetes mellitus), and previous severe laboratory-confirmed coronavirus disease 2019 history.
Article
Biology
Seda Arslan Tuncer, Hakan Ayyildiz, Mehmet Kalayci, Taner Tuncer
Summary: The study introduces a 25-depth CNN model called Scat-NET using scattergram images to detect COVID-19, achieving an overall accuracy of 92.4%. It is proposed as an alternative to CT scans and a secondary test for patients with negative RT-PCR results to improve detection accuracy.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Waleed M. M. El-Sayed, Alli L. Gombolay, Penghao Xu, Taehwan Yang, Youngkyu Jeon, Sathya Balachander, Gary Newnam, Sijia Tao, Nicole E. Bowen, Tomas Bruna, Mark Borodovsky, Raymond F. Schinazi, Baek Kim, Yongsheng Chen, Francesca Storici
Summary: The distribution of rNMPs in different organelle genomes of Chlamydomonas reinhardtii reveals specific biases and patterns, reflecting the varying ATP levels present in the algal cells. Researchers identified the orthologous genes for all three subunits of the RNase H2 enzyme in the algal organelles using different tools and protocols.
Article
Neurosciences
Wenyuan Ji, Xuan Zhe, Lusheng Li, Yang Cheng, Xueling Zhao, Ping Liang, Chunxi Long, Jianjun Zhou
Summary: miR-137 is down-regulated in medulloblastoma tissues and cells, and its down-regulation is significantly associated with poor prognosis and clinical indicators. Up-regulated miR-137 inhibits cell proliferation, migration, invasion, cell cycle progression, and induces apoptosis by targeting KDM1A. MiR-137 may serve as a prognostic biomarker for medulloblastoma.
NEUROMOLECULAR MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Huina Wu, Mingyuan Zou, Xiaobo Fan, Feiya Su, Feng Xiao, Meiling Zhou, Yan Sun, Fengfeng Zhao, Guoqiu Wu
Summary: A catalytic hairpin assembly (CHA) combined with lateral flow immunoassay (CHA-LFIA) method was proposed for rapid and precise detection of influenza viruses and RSV, demonstrating high sensitivity and specificity.
Article
Biotechnology & Applied Microbiology
Wayne M. Barnes, Zhian Zhang, Milko B. Kermekchiev
Summary: Changing an amino acid of Taq DNA polymerase leads to faster PCR; surprising results from negative controls reveal additional abilities of the enzyme.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Pathology
Angela Avenoso, Salvatore Campo, Michele Scuruchi, Manuela Mania, Vanessa Innao, Angela D'Ascola, Giuseppe Mandraffino, Andrea G. Allegra, Caterina Musolino, Alessandro Allegra
Summary: MGUS is a pre-malignant abnormality of plasma cells characterized by increased serum immunoglobulin levels. This study evaluated microRNA expression in peripheral lymph-monocytes of MGUS subjects, discovering a signature of miRNAs involved in regulating genes related to lymphocyte homeostasis, cell proliferation, apoptosis, and MM progression. These findings suggest potential new therapeutic strategies for monoclonal gammopathies.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Oncology
Jiu-Ning Huang, Hui -Ming Zhang, Jun-Dong Cai, Wu-Long Wang, Ping Wang
Summary: Emerging evidence indicates the important role of long noncoding RNAs (lncRNAs) in cancer tumorigenesis and development, suggesting their potential as therapeutic targets. Through bioinformatic analysis, a ceRNA network was constructed, and the DSCR8/miR-22-3p/ARPC5 axis was identified as a potential target in liver cancer. Further investigation revealed that DSCR8 is highly expressed in liver cancer cell lines Hep3B and Huh7, and inhibition of DSCR8 suppresses cell proliferation and promotes apoptosis. Moreover, DSCR8 functions as a sponge for miR-22-3p, and ARPC5 is a target gene of miR-22-3p. The study suggests that lncRNA_DSCR8 promotes liver cancer progression and inhibits apoptosis through the miR-22-3p/ARPC5 axis, highlighting DSCR8 as a potential therapeutic target for liver cancer.
Article
Agronomy
Hyo-Jeong Lee, Sena Choi, In-Sook Cho, Ju-Yeon Yoon, Rae-Dong Jeong
Summary: In this study, a sensitive and accurate quantification method for PlAMV infection in lily samples using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay was established. The RT-ddPCR assay showed high specificity and sensitivity for PlAMV detection and quantification, outperforming the traditional RT-qPCR method. Field validation tests further confirmed the superior sensitivity of RT-ddPCR. This method can be used for PlAMV infection diagnosis, especially in plant quarantine inspection and PlAMV-free certification programs.
Article
Oncology
Tian Li, Dong Guo, Xiaoyan Xu, Peng Liu, Ping Wang, Yongcun Zhu, Lin Lin, Yemin Qu, Feng Liu, Yanliu Chu, Xiaozhong Gao
Summary: MicroRNA-153 (miR-153), a novel tumor-related miRNA, is aberrantly expressed in gastric cancer (GC). The study showed that miR-153 expression is significantly decreased in GC cells, tissues, and serum samples. Transfection with miR-153 mimics inhibits GC cell proliferation and migration, while promoting apoptosis. The serum expression levels of miR-153 are lower in patients with larger tumor size, poor differentiation, lymph node metastasis, and advanced tumor stage. These findings suggest that miR-153 may serve as a potential tumor biomarker and therapeutic target in GC.
Review
Biochemistry & Molecular Biology
Adrianna Kondracka, Paulina Gil-Kulik, Bartosz Kondracki, Karolina Fraszczak, Anna Oniszczuk, Magda Rybak-Krzyszkowska, Jakub Staniczek, Anna Kwasniewska, Janusz Kocki
Summary: MicroRNAs are non-coding segments of RNA that modulate various biological processes. Their presence in biological fluids like blood, saliva, tears, and breast milk has raised interest in their potential impact on health and disease. Although hundreds of microRNAs have been found in breast milk, evidence on their function remains inconsistent. This scoping review aims to map the evidence on the occurrence, characterization techniques, and functional roles of microRNAs in breast milk. While methodological challenges need to be addressed, significant progress has been made in characterizing the microRNA profiles of human breast milk.
