Nocardioides nanhaiensis sp nov., an actinobacterium isolated from a marine sediment sample

A rod- to coccus-shaped, non-spore-forming actinobacterium, strain YIM M13091(T), was isolated from a marine sediment sample collected from the South China Sea and examined by a polyphasic approach to clarify its taxonomic position. This Gram-staining-positive, aerobic actinobacterium did not produce substrate mycelium and aerial hyphae, and no diffusible pigments were produced on the media tested. The optimum growth occurred at 30 degrees C, 1 % (w/v) NaCl and pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Nocardioides, with low levels (<= 96.2%) of sequence similarity with respect to Nocardioides kribbensis KSL-2(T) and other members of the genus Nocardioides. Whole-organism hydrolysates of the strain contained LL-2,6-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H-4), with MK-8 in a minor amount. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, hydroxphosphatidylinositol and phosphatidylcholine, were the main polar lipids detected, while iso-C-16:0 and C-18:1 omega 9c were the major fatty acids. The G+C content of the genomic DNA was 68.5 mol%. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a member of the genus Nocardioides, and the name Nocardioides nanhaiensis sp. nov. (Type strain YIM M13091(T)=JCM 18127(T)=CCTCC AA 2011020(T)) is proposed for the novel species.