Brenneria populi sp nov., isolated from symptomatic bark of Populusxeuramericana canker

Five Gran-stain-negative, facultatively anaerobic, motile, bacterial strains were isolated from symptomatic bark tissue of Populusxeuramericana canker. Strains grew at 4-41 degrees C, pH 4-10 and 0-6% (w/v) salinity. They were positive with respect to catalase activity and negative for oxidase activity, nitrate reduction and the Voges-Proskauer reaction. Analysis of 16S rRNA gene sequences indicated that these five poplar isolates belong to the genus Brenneria, having highest sequence similarity of 95.98 % with Brenneria goodwinii LMG 26270(T). These five isolates formed a single cluster based on multilocus sequence analysis, indicating that they all belong to a single taxon within the genus Brenneria, which was confirmed by DNA-DNA hybridization. The DNA G+C content was 54.9-55.7 mol%, and the main fatty acids were C-16:0, C-18:1 omega 7C, C-17:0 cyclo and C-16:1 omega 7c/iso-C-15:0 2-OH. Based on these results, we describe a novel species of the genus Brenneria with the proposed name Brenneria populi sp. nov. The type strain is D9-5(T)(=CFCC 11963(T)=KCTC 42088(T)).