Nocardioides salsibiostraticola sp nov., isolated from biofilm formed in coastal seawater

A Gram-staining-positive, non-motile, aerobic, non-spore-forming and short rod-shaped bacterial strain, PAMC 26527(T), was isolated from biofilm formed in coastal seawater of the Norwegian Sea. Analysis of the 16S rRNA gene sequence of strain PAMC 26527(T) revealed a clear affiliation with the genus Nocardioides. Based on phylogenetic analysis, strain PAMC 26527(T) showed the closest phylogenetic relationship with Nocardioides caricicola YC6903(T) with 16S rRNA gene sequence similarity of 96.3 %. Strain PAMC 26527(T) grew in the presence of 0-5.0% sea salts. The optimum temperature and pH for growth were 20 degrees C and pH 7.5. The major cellular fatty acids of strain PAMC 26527(T) were iso-C-16:0, C-17:1 omega 8c and C-18:1 omega 9c and the major isoprenoid quinone was MK-8(H-4). Cell-wall analysis showed that strain PAMC 26527(T) contained LL-diaminopimelic acid. The genomic DNA G + C content was 69.3 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain PAMC 26527(T) could be clearly distinguished from recognized members of the genus Nocardioides. Thus, strain PAMC 26527(T) should be classified as representing a novel species in the genus Nocardioides, for which the name Nocardioides salsibiostraticola sp. nov. is proposed. The type strain is PAMC 26527(T) (=KCTC 29158(T)=JCM 18743(T)).