Paraperlucidibaca wandonensis sp nov., isolated from seawater, and emended description of the genus Paraperlucidibaca Oh et al 2011

A Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated WT-RY4(T), was isolated from wood falls in the South Sea, South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain WT-RY4(T) grew optimally at 25 degrees C, at pH 7.0-7.5 and in the absence of NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WT-RY4(T) clustered with the type strain of Paraperlucidibaca baekdonensis with a bootstrap resampling value of 100%. Strain WT-RY4(T) exhibited 16S rRNA gene sequence similarity values of 98.8% and 96.3% to Paraperlucidibaca baekdonensis RL-2(T) and Perlucidibaca piscinae IMCC 1704(T), respectively and less than 91.5% to the type strains of other species used in the phylogenetic analysis. The DNA G+C content of strain WT-RY4(T) was 52.4 mol% and the mean DNA-DNA relatedness value with Paraperlucidibaca baekdonensis RL-2(T) was 25%. Strain WT-RY4(T) contained Q-11 as the predominant ubiquinone and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c, C-16:0 and C-12:0 3-OH as the major fatty acids. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain WT-RY4(T) was distinguishable from Paraperlucidibaca baekdonensis RL-2(T). On the basis of the data presented, strain WT-RY4(T) is considered to represent a novel species of the genus Paraperlucidibaca, for which the name Paraperlucidibaca wandonensis sp. nov. is proposed. The type strain is WT-RY4(T) (=KCTC 32216(T)=CCUG 63419(T)). An emended description of the genus Paraperlucidibaca is also provided.