Pseudonocardia sediminis sp nov., isolated from marine sediment

A Gram-stain-positive, aerobic actinomycete, designated strain YIM M13141(T), was isolated from a marine sediment sample from the South China Sea, and its taxonomic position was determined using a polyphasic approach. The strain produced branched substrate mycelium and aerial hyphae, but no diffusible pigments were produced on the media tested. At maturity, substrate mycelium was fragmented and spore chains were formed on aerial hyphae and substrate mycelium. Optimum growth occurred at 28 degrees C, 1-3% (w/v) NaCl and pH 7.0. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the genus Pseudonocardia, showing highest levels of similarity with respect to Pseudonocardia sichuanensis KLBMP 1115(T) (97.1%), Pseudonocardia tetrahydrofuranoxydans K1(T) (97.1 %) and Pseudonocardia kunmingensis YIM 63158(T) (97.0%). Whole-organism hydrolysates of the strain contained meso-diaminopimelic acid and the sugars galactose, glucose, mannose and arabinose. The predominant menaquinone was MK-8(H-4). The polar lipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylethanolamine, two unknown phosphoglycolipids and two glycolipids. The major fatty acid was iso-C-16:0. The G+C content of the genomic DNA was 73.1 molok. DNA DNA relatedness with P. tetrahydrofuranoxydans DSM 44239(T) was 42.8 +/- 3.5 % (mean +/- SD). Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia sediminis sp. nov. is proposed. The type strain is YIM M13141(T) (=DSM 45779(T)=JCM 18540(T)).