Pseudorhodobacter wandonensis sp nov., isolated from wood falls, and emended description of the genus Pseudorhodobacter

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated WT-MW11(T), was isolated from wood falls on the coast of Wando, an island of South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain WT-MW11(T) grew optimally at pH 7.0-8.0, at 25 degrees C and in the presence of 2.0-3.0?% (w/v) NaCl. Bacteriochlorophyll a was not produced. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WT-MW11(T) clustered with Pseudorhodobacter ferrugineus IAM 12616(T) and Pseudorhodobacter aquimaris HDW-19(T), with which it exhibited 16S rRNA gene sequence similarity values of 97.9 and 95.5%, respectively. Strain WT-MW11(T) contained Q-10 as the predominant ubiquinone and C-18:1 omega 7c as the major fatty acid. The polar lipid profile of strain WT-MW11(T) was similar to those of P. ferrugineus JCM 20687(T) and P. aquimaris HDW-19(T). The DNA G+C content of strain WT-MW11(T) was 61.6 mol% and its mean DNA-DNA relatedness value with P. ferrugineus JCM 20687(T) was 12.3%. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WT-MW11(T) was distinguishable from the two recognized species of the genus Pseudorhodobacter. On the basis of the data presented, strain WT-MW11(T) is considered to represent a novel species of the genus Pseudorhodobacter, for which the name Pseudorhodobacter wandonensis sp. nov. is proposed. The type strain is WT-MW11(T) (=KCTC 23672(T)=CCUG 61506(T)). The description of the genus Pseudorhodobacter is emended.