Nocardioides iriomotensis sp nov., an actinobacterium isolated from forest soil

An actinomycete strain, designated IR27-S3(T), was isolated from a forest soil sample collected from Iriomote Island, Okinawa, Japan. Cells of the isolate were Gram-stain-positive, aerobic, non-sporulating, non-motile coccoids or short rods. The strain grew in the presence of 0-7% (w/v) NaCl, at pH 6-8 and at 12-37 degrees C, with optimum growth at 30 degrees C. Chemotaxonomically, the strain contained LL-diaminopimelic acid as the diagnostic diamino acid and MK-8(H-4) as the predominant menaquinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The major cellular fatty acids were iso-C-16:0, C-17:1 cis-9, C-15:0 and iso-C-15:0. The DNA G+C content was 73.7 molok. On the basis of 16S rRNA gene sequence analysis, strain IR27-S3(T) was closely related to Nocardioides mesophilus MSL-22(T) (98.1 % 16S rRNA gene sequence similarity), Marmoricola bigeumensis MSL-05(T) (97.2%) and Nocardioides jensenii DSM 20641(T) (96.5 %). On the basis of fatty acid analysis, phylogenetic analysis and phenotypic data, the isolate should be classified in a novel species of the genus Nocardioides, for which the name Nocardioides iriomotensis sp. nov. is proposed. The type strain is IR27-S3(T) (=NBRC 105384(T) =KACC 14926(T)).