Citreicella marina sp. nov., isolated from deep-sea sediment

A taxonomic study was carried out on a novel strain, designated CK-I3-6(T), which was isolated from deep-sea sediment of the south-west Indian Ocean Ridge. Cells were Gram-reaction-negative, oxidase- and catalase-positive, rod-shaped and non-motile. Growth was observed at 4-38 degrees C and in 1-12 % (w/v) NaCl. Cells were able to degrade gelatin and oxidize thiosulfate but did not reduce nitrate. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CK-I3-6(T) belonged to the genus Citreicella with a sequence similarity of 97.3% to Citreicella thiooxidans CHLG 1(T), while similarities with other taxa were < 95.7 %. DNA-DNA hybridization showed that strain CK-I3-6(T) and C. thiooxidans CHLG 1(T) showed a low DNA-DNA relatedness (48 +/- 3 %). The principal fatty acids were C-16:0 (7.8%), C-18:1 omega 7c (66.6%), summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c; 6.3%) and C-19:0 omega 8c cyclo (10.0%). The chromosomal DNA G + C content was 67.5 mol%. On the basis of the combined genotypic and phenotypic data, strain CK-I3-6(T) represents a novel species of the genus Citreicella, for which the name Citreicella marina sp. nov. is proposed. The type strain is CK-I3-6(T) (=CCTCC AB 209064(T) =LMG 25230(T) =MCCC 1A03060(T)).