Saccharopolyspora tripterygii. sp nov., an endophytic actinomycete isolated from the stem of Tripterygium hypoglaucum

An endophytic actinomycete, designated strain YIM 65359(T), was isolated from a surface-sterilized stem sample of Tripterygium hypoglaucum collected from Yunnan province, south-west China. The morphological and chemotaxonomic properties of the new isolate were consistent with those of members of the genus Saccharopolyspora. Analysis of 16S rRNA gene sequences revealed that the new isolate was most closely related to 'Saccharopolyspora endophytica' YIM 61095 (98.6%), Saccharopolyspora flava AS4.1520(T) (97.6%) and Saccharopolyspora spinosa DSM 44228(T) (97.0%). The results of DNA-DNA hybridizations (57.5%, 44.9% and 48.5%, respectively) with the above micro-organ isms, in combination with differences in the biochemical and physiological characteristics, suggested that strain YIM 65359(T) should be classified as a novel species of the genus Saccharopolyspora. The name Saccharopolyspora tripterygii sp. nov. is proposed for this novel species, with YIM 65359(T) (=CCTCC AA 208062(T) =DSM 45269(T)) as the type strain.