Parapusillimonas granuli gen. nov., sp nov., isolated from granules from a wastewater-treatment bioreactor

A novel betaproteobacterium, designated strain Ch07(T), was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in South Korea. In order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. The new strain was Gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16S rRNA gene sequence analysis revealed that strain Ch07(T) belonged to the class Betaproteobacteria, being related to Pusillimonas noertemannii BN9(T) (gene sequence similarity 97.30%), Achromobacter xylosoxidans subsp. xylosoxidans DSM 10346(T) (97.09%), Bordetella pertussis DSM 5571(T) (97.01%), Pigmentiphaga kullae DSM 13608(T) (96.68%) and Castellaniella defragrans DSM 1214(T) (96.47%). The results of DNA-DNA hybridization tests showed that reassociation values were less than 62% with respect to these closely related type strains. Chemotaxonomic data showed that strain Ch07(T) possessed ubiquinone Q-8. The G + C content of the genomic DNA was 67.9 +/- 0.1 mol%. The major polyamine of strain Ch07(T) was putrescine. The major polar lipids of strain Ch07(T) were phosphatidylethanolamine, followed by diphosphatidylglycerol and phosphatidylglycerol. When strain Ch07(T) was incubated on tryptic soy agar, the major cellular fatty acids were C-16:0, C-17:0 cyclo, summed feature 3 (C-16.1 omega 7c/iso C-15:0 2-OH) and summed feature 5 (C-18:1 omega 7c/omega 9t/omega 12t). The results of DNA-DNA hybridizations, in combination with the chemotaxonomic and physiological data, demonstrated that strain Ch07(T) represents a novel species of a new genus, for which the name Parapusillimonas granuli gen. nov., sp. nov. is proposed. The type strain of the type species is Ch07(T) (=KCTC 12668(T)=LMG 24012(T)).