Nocardioides fonticola sp nov., a novel actinomycete isolated from spring water

A Gram-positive, rod-shaped, yellow-pigmented actinomycete, designated strain NAA-13(T), was isolated from a freshwater spring located in Kaoshiung, southern Taiwan. This strain was characterized using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Nocardioides. The strain contained LL-diaminopimelic acid as the principal diamino acid of the peptidoglycan, MK-8(H-4) as the major menaquinone, and iso-C-16:0 and C-17:0 as the predominant fatty acids. 16S rRNA gene sequence studies showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Nocardioides; its closest neighbours were Nocardioides insulae DS-51(T) (94.9 % similarity), Nocardioides pyridinolyticus KCTC 0074BP(T) (96.5 % similarity) and Nocardioides aquiterrae GW-9(T) (96.6% similarity). The DNA G+C content of strain NAA-13(T) was 71.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain NAA-13(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides fonticola sp. nov. is proposed. The type strain is NAA-13(T) (=LMG 24213(T) = BCRC 16874(T)).