Article
Multidisciplinary Sciences
Jing Wang, Steven Randolph, Qian Wu, Aurelien Botman, Jenna Schardt, Cedric Bouchet-Marquis, Xiaolin Nan, Chad Rue, Marcus Straw
Summary: A CLEM workflow based on a novel FIB/SEM technology using an OSSM process was proposed, which achieved high resolution and imaging efficiency while improving throughput. By combining with iPALM technique, successful 3D imaging of mitochondria was carried out, demonstrating the advantages of OSSM-SEM in CLEM applications.
SCIENTIFIC REPORTS
(2021)
Article
Materials Science, Multidisciplinary
Zhongquan Jiang, Danqing Sun, Huize Guan, Yutong Sun, Menglei Ye, Lin Zhang, Tingting Gu, Jiani Chen, Shujie Wang, Chunhua Zhang, Shimei Wang, Zhenlei Zhou, Ying Ge, Zhen Li
Summary: The ULLS technique offers a novel approach for preparing ultra-thin sections for TEM analysis, simplifying the sample preparation process and providing acceptable toxicity levels for most microbial cells. It holds promise for playing a significant role in studying the ultrastructure of microbial cells.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Cell Biology
Pieter Baatsen, Sergio Gabarre, Katlijn Vints, Rosanne Wouters, Dorien Vandael, Rose Goodchild, Sebastian Munck, Natalia V. Gounko
Summary: In life science research, it is important to determine the location of molecules within cells or tissues, typically using genetically encoded fluorescent proteins and fluorescence affinity-labeling. To add an ultrastructural context, correlative light and electron microscopy (CLEM) imaging can be used, which preserves both fluorescent signals and provides high-resolution electron microscopy imaging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Virology
Michael Laue, Tobias Hoffmann, Janine Michel, Andreas Nitsche
Summary: In this study, a new method was used to visualize and analyze the ultrastructure of coronavirus particles and infected host cells in patient swab samples. The results showed that SARS-CoV-2 particles could be detected in the upper airways of patients and within host cells, providing important insights into the pathogenicity of the virus.
Article
Microbiology
Maximilian W. D. Raas, Thiago P. Silva, Jhamine C. O. Freitas, Lara M. Campos, Rodrigo L. Fabri, Rossana C. N. Melo
Summary: The study utilized whole slide imaging (WSI) to visualize Candida biofilm formation, staining accumulated biofilms with fluorescent markers and scanning in both bright-field and fluorescence modes using a WSI digital scanner. WSI allowed for clear assessment of biofilm size and structural features, with quantitative analysis showing reductions in biofilm-covered surface area upon antifungal exposure. At the single-cell level, WSI proved to be adequate for evaluating morphometric parameters, demonstrating its reliability in visualizing Candida biofilms and making it an important addition to microscopic tools for fungal biofilm growth analysis.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Chemistry, Physical
Sapna Sinha, Jamie H. Warner
Summary: Transmission electron microscopy (TEM) is a vital tool for studying nanomaterials at the atomic level, with graphene playing a key role in facilitating the characterization of other materials and understanding their properties and interactions. The review highlights the use of TEM with graphene as a substrate for studying a wide range of nanomaterials, discussing the challenges and potential engineering applications in the future.
Review
Immunology
Desalegn Tadesse Egu, Thomas Schmitt, Jens Waschke
Summary: This study characterized the effects of PV-IgG on desmosome ultrastructure in a human ex vivo skin model using transmission electron microscopy. Results showed that inhibition of specific signaling pathways could protect human epidermis from blistering. The study also revealed the importance of desmosome number and keratin filament insertion in preventing skin blistering.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hong-Lim Kim, Tae-Ryong Riew, Jieun Park, Youngchun Lee, In-Beom Kim
Summary: Researchers have developed a new method by combining light and electron microscopy with immunolabeling, to overcome technical barriers in immuno-electron microscopy. This method allows for excellent antigenicity of target proteins and preservation of ultrastructure, without the need for specifically designed correlative light and electron microscopy devices. It can be easily applied in many biomedical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Marina Gotelli, Elsa Lattar, Lucia Melisa Zini, Sonia Rosenfeldt, Beatriz Galati
Summary: The appearance of new cellular structures and characteristics in the tapetum suggests that there is still much to discover about its functions. The ultrastructure of the tapetum provides important information for understanding its functions. This review aims to collect detailed information about the tapetum ultrastructure for future research.
Article
Veterinary Sciences
Hadiseh Dadras, Faranak Dadras, Aiman Imentai, Oleksandr Malinovskyi, Tomas Penka, Jitka Kolarova, Tomas Policar
Summary: This study examined the ultrastructure of spermatogenic stages and mature spermatozoa in Thymallus thymallus. The testes were observed using a transmission electron microscope to investigate the structure and morphology of germ cells, spermatozoa, and somatic cells. The findings provide valuable information for understanding germ cell development and grayling breeding practices.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Cell Biology
Helmut Hopfer, Martin C. Herzig, Rainer Gosert, Thomas Menter, Jurgen Hench, Alexandar Tzankov, Hans H. Hirsch, Sara E. Miller
Summary: Transmission electron microscopy is a valuable tool for investigating tissues of COVID-19 patients to visualize SARS-CoV-2, but the presence of 'virus-like particles' in organs has been controversial. Our review aims to discuss ultrastructural changes associated with SARS-CoV-2 infection and COVID-19, and how viral replication induces extensive remodeling of intracellular membrane systems. Identifying coronavirus in tissues by transmission electron microscopy remains challenging.
Article
Anatomy & Morphology
Xiu Zhu, Sai Liu, Chang-Qing Xu, Kun Guo, Rong Xu, Hai-Li Qiao, Jun Chen
Summary: The study examined the external morphologies and distributions of sensilla on the antennae and ovipositors of P. jaapiellae using scanning electron microscopy. It identified nine morphological sensilla types on the antennae and six types on the ovipositor, discussing their potential biological functions. These findings lay the foundation for further research on chemical communication between P. jaapiellae and their host, and the development of a biological control program for G. lycantha.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Anatomy & Morphology
Sri Widiyanti Rahayu Hilia, Abinawanto, Astari Dwiranti, Anom Bowolaksono, Retno Lestari, Fadhilah, Anang Hari Kristanto, Nobuko Ohmido
Summary: The ultrastructure of spermatozoa of Javaen barb fish (S. orphoides) was studied using transmission and scanning electron microscopy. The spermatozoa of S. orphoides are relatively simple cells composed of a spherical head, a short midpiece, and a flagellum. The ultrastructure analysis showed the absence of acrosome, the total length of spermatozoa, and the specific organelles present. This study provides important information for understanding the reproductive biology and conservation of S. orphoides.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Biochemical Research Methods
Tong Xin, Yanan Lv, Haoran Chen, Linlin Li, Lijun Shen, Guangcun Shan, Xi Chen, Hua Han
Summary: This article proposes a new method for serial section registration using an unsupervised optical flow network to measure feature similarity and eliminate nonlinear deformation. The optical flow network is also used to estimate and compensate for cumulative registration error, enabling the reconstruction of biological tissue structures. Experimental results demonstrate that this method effectively improves the spatial continuity of serial sections and enhances the accuracy of registration and reconstruction for biological tissue structures.
Review
Anatomy & Morphology
Aseem Grover, Reshma Sinha, Divya Jyoti, Caterina Faggio
Summary: The development of electron microscope (EM) in 1931 revolutionized microscopical examination of both biological and non-biological samples. Modifications in EM techniques have widened their applicability in various sectors, such as drug toxicity understanding, criminal investigation, and nano-molecule characterization. EM plays an important role in toxicity assessment and disease prognosis, especially in understanding the impact of toxicants at the ultra-structural level in biological systems.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
