Article
Biochemistry & Molecular Biology
Yilong Zhao, Huajun Yang, Wei Shan, Anchen Guo, Qun Wang
Summary: Moderate ethanol preconditioning protects against ischemic brain injury by upregulating the expression of BKCa channels in rats.
NEUROCHEMICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Kemeng Zhao, Pengwei Wang, Xiaoguang Tang, Na Chang, Haonan Shi, Longfei Guo, Bingyi Wang, Pengfei Yang, Tiantian Zhu, Xinghua Zhao
Summary: Stroke is caused by ruptured or blocked cerebral blood vessels and subsequent disruption of brain blood circulation, resulting in rapid neurological deficits. The majority of stroke cases are ischemic strokes. Current treatments for ischemic stroke, such as t-PA thrombolytic therapy and surgical thrombectomy, can paradoxically lead to ischemia-reperfusion injury, exacerbating brain damage. Minocycline, a semi-synthetic tetracycline antibiotic, has demonstrated a wide range of neuroprotective effects independent of its antibacterial activity. In this article, we summarize the mechanisms underlying the protective effects of minocycline against cerebral ischemia-reperfusion injury, including modulation of oxidative stress, inflammatory response, excitotoxicity, programmed cell death, and blood-brain barrier injury. We also discuss the role of minocycline in alleviating stroke-related complications, providing a theoretical basis for its clinical application in cerebral ischemia-reperfusion injury.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Clinical Neurology
Lukasz Przykaza, Ewa Kozniewska
Summary: Ischemic stroke is a major global health issue with limited treatment options. Current treatments focus on reperfusion methods, but a small percentage of patients are eligible. Basic research suggests that neuropeptide Y activation may have potential protective effects against stroke.
TRANSLATIONAL STROKE RESEARCH
(2022)
Article
Neurosciences
Reai Shan, Hongyan Zhou, Xinfang Liu, Guangjun Su, Guangsen Liu, Xiaoli Zhang, Cong Sun, Zining Yu, Lifang Zhan, Zhihua Huang
Summary: This study evaluated the protective effects of different fluids on the blood-brain barrier in cerebral ischaemia/reperfusion injury, and the results showed that HSH and other fluids could alleviate neurological deficits, brain infarction, brain oedema, and increased BBB permeability.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Shraddha Tipirneni, Peter Stanwell, Robert Weissert, Sonu M. M. Bhaskar
Summary: This meta-analysis highlights a significant association between CMBs and adverse post-procedural safety outcomes in AIS patients undergoing reperfusion therapy, including symptomatic intracerebral hemorrhage, hemorrhagic transformation, poor functional outcomes, and increased mortality. The notable prevalence of CMBs in both the overall AIS population and those undergoing reperfusion therapy emphasizes their importance in post-stroke prognostication.
Article
Biochemical Research Methods
Di Wu, Jian Chen, Longfei Wu, Hangil Lee, Jingfei Shi, Mo Zhang, Yanhui Ma, Xiaoduo He, Zixin Zhu, Feng Yan, Chuanjie Wu, Yunxia Duan, Yongjuan Fu, Sijie Li, Xinglong Zhi, Xuxiang Zhang, Shengli Li, Yuchuan Ding, Xunming Ji
Summary: The article describes a protocol for developing a stroke model in rhesus monkeys and highlights the advantages of using this model in simulating patients with ischemic stroke.
Article
Clinical Neurology
Alis J. Dicpinigaitis, Steven D. Shapiro, Rolla Nuoman, Haris Kamal, Philip Overby, Gurmeen Kaur, Ji Y. Chong, Johanna T. Fifi, Neha Dangayach, Eliza C. Miller, Shadi Yaghi, Fawaz Al-Mufti
Summary: Population-level data from the United States over a twenty-year period show that intravenous thrombolysis treatment for pediatric AIS patients is associated with high rates of favorable outcomes without an increased risk of hemorrhagic transformation.
INTERNATIONAL JOURNAL OF STROKE
(2023)
Review
Biochemistry & Molecular Biology
Shannon M. Stuckey, Lin Kooi Ong, Lyndsey E. Collins-Praino, Renee J. Turner
Summary: Ischaemic stroke is a leading cause of death and disability worldwide, affecting over 17 million people each year. Neuroinflammation and immune mediators play a crucial role in acute and long-term neuronal tissue damage and healing following stroke, with specific spikes in neuroinflammation observed in sites of secondary neurodegeneration. However, the exact mechanisms driving stroke-induced neuroinflammation remain poorly understood, leading to a lack of effectiveness in current anti-inflammatory treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Thijs R. J. Landman, Yvonne Schoon, Michiel C. Warle, Frederick J. A. Meijer, Frank-Erik De Leeuw, Dick H. J. Thijssen
Summary: This study found no significant improvement in infarct size or clinical outcome in patients with an acute ischemic stroke who were treated with repeated remote ischemic postconditioning.
INTERNATIONAL JOURNAL OF STROKE
(2023)
Article
Clinical Neurology
Longfei Wu, Ming Wei, Bohao Zhang, Bowei Zhang, Jian Chen, Sifei Wang, Leilei Luo, Shuling Liu, Sijie Li, Changhong Ren, David C. Hess, Haiqing Song, Wenbo Zhao, Xunming Ji
Summary: This study investigated the safety and tolerability of direct ischemic postconditioning (IPostC) in both a dog model and patients with acute ischemic stroke treated with thrombectomy, and found it to be safe, feasible, and tolerable.
Article
Neurosciences
Hongjie Xi, Tao Tao, Ruru Zhang, Xinxin Xue, Yana Zhu, Jiuyang Liu, Xianyi Xin, Xianzhang Zeng
Summary: This study demonstrates that the selective I2-imidazoline receptor ligand 2BFI can alleviate the severity of ischemic stroke in diabetic patients by reducing microglial NOX2 production, inhibiting inflammatory and oxidative stress responses, and decreasing neuron death.
BRAIN RESEARCH BULLETIN
(2021)
Article
Clinical Neurology
Yinghua Jiang, Yang Ji, Iris Yuwen Zhou, Ning Liu, Phillip Zhe Sun, Mingming Ning, Aaron S. Dumont, Xiaoying Wang
Summary: The study found that LT3001, a novel synthetic small molecule, has thrombolytic and free radical scavenging activities and may serve as an alternative thrombolytic for stroke treatment. Results showed that LT3001 administered at 1.5 hours after stroke had neuroprotective effects, while treatment at 3 hours after stroke yielded better outcomes in terms of reducing infarct size, swelling rate, and hemorrhagic transformation compared to tPA. LT3001 did not interfere with tPA activity and had no significant impact on related serum levels. Therefore, LT3001 shows promise as a novel therapy for thrombotic ischemic stroke.
TRANSLATIONAL STROKE RESEARCH
(2022)
Article
Clinical Neurology
Estelle Pruvost-Robieux, Joseph Benzakoun, Guillaume Turc, Angela Marchi, Rossella Letizia Mancusi, Catherine Lamy, Valerie Domigo, Catherine Oppenheim, David Calvet, Jean-Claude Baron, Jean-Louis Mas, Martine Gavaret
Summary: The study aimed to evaluate the feasibility and safety of C-tDCS in reducing infarct growth in hyperacute middle cerebral artery stroke patients. While no significant difference was found between active and sham groups, there was a potential benefit of C-tDCS in patients with high NIH Stroke Scale score or large vessel occlusion. Further randomized trials targeting these populations may be warranted.
Article
Cardiac & Cardiovascular Systems
Alexandra J. Lansky, Rajendra Makkar, Tamim Nazif, Steven Messe, John Forrest, Rahul Sharma, Joachim Schofer, Axel Linke, David Brown, Abhijeet Dhoble, Phillip Horwitz, Ming Zang, Frederico DeMarco, Vivek Rajagopal, Michael G. Dwyer, Robert Zivadinov, Pieter Stella, Joshua Rovin, Helen Parise, Susheel Kodali, Andreas Baumbach, Jeffrey Moses
Summary: The REFLECT I trial investigated the safety and effectiveness of the TriGuard(TM) HDH(TG) cerebral embolic deflection device in patients undergoing transcatheter aortic valve replacement. The study enrolled 258 patients, with the primary safety outcome being met but the primary efficacy endpoint not being met. TG was found to be safe compared to historical TAVR data, but did not meet the predefined effectiveness endpoint compared with unprotected TAVR controls.
EUROPEAN HEART JOURNAL
(2021)
Review
Clinical Neurology
E. M. Vos, V. J. Geraedts, A. van der Lugt, D. W. J. Dippel, M. J. H. Wermer, J. Hofmeijer, A. C. G. M. van Es, Y. B. W. E. M. Roos, C. M. P. C. D. Peeters-Scholte, I. R. van den Wijngaard
Summary: Neuroprotective agents in combination with reperfusion therapy have shown some efficacy in acute ischemic stroke, but relevant studies are limited. Future research should use standardized outcome measures and combine neuroprotective agents with reperfusion therapy.
FRONTIERS IN NEUROLOGY
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Paloma Jorda, Daniel Martinez, Marta Farrero, Maria Angeles Marcos, Elena Sandoval, Maria Angeles Castel, Daniel Pereda, Eduard Quintana, Elena Arbelo, Aurora Sanchez, Oscar Campuzano, Coloma Tiron de Llano, Ramon Brugada, Josep Brugada, Manuel Castella, Felix Perez-Villa, Ana Garcia-alvarez
CIRCULATION-HEART FAILURE
(2022)
Article
Biochemistry & Molecular Biology
Rocio Toro, Alexandra Perez-Serra, Alipio Mangas, Oscar Campuzano, Georgia Sarquella-Brugada, Maribel Quezada-Feijoo, Monica Ramos, Martin Alcala, Esther Carrera, Carlos Garcia-Padilla, Diego Franco, Fernando Bonet
Summary: Oxidative stress plays a significant role in cardiovascular diseases, and endoplasmic reticulum stress is an important source of reactive oxygen species. miR-16-5p could promote oxidative stress through ER stress induction and regulation of ATF6. Analysis of oxidative markers in the plasma of ischemic dilated cardiomyopathy patients showed a correlation with oxidative stress. Overexpression of miR-16-5p increased oxidative stress in cardiomyoblasts, while its suppression decreased apoptosis, inflammation, and cardiac damage through activation of the ATF6-mediated cytoprotective pathway. ATF6 was identified as a direct target gene of miR-16-5p.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Matthieu Chourrout, Margaux Roux, Carlie Boisvert, Coralie Gislard, David Legland, Ignacio Arganda-Carreras, Cecile Olivier, Francoise Peyrin, Herve Boutin, Nicolas Rama, Thierry Baron, David Meyronet, Emmanuel Brun, Hugo Rositi, Marlene Wiart, Fabien Chauveau
Summary: This study demonstrates the use of ethanol-induced brain sample dehydration and XPCT for the detection and morphometric analysis of A beta plaques in the hippocampus. The proposed workflow identified differences in signal intensity and 3D shape parameters among different Alzheimer mouse strains.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Matthieu Chourrout, Hugo Rositi, Elodie Ong, Violaine Hubert, Alexandre Paccalet, Louis Foucault, Awen Autret, Barbara Fayard, Cecile Olivier, Radu Bolbos, Francoise Peyrin, Claire Crola-da-Silva, David Meyronet, Olivier Raineteau, Helene Elleaume, Emmanuel Brun, Fabien Chauveau, Marlene Wiart
Summary: This study proposes a new approach for mapping myelin fibers in the whole rodent brain with microscopic resolution without strenuous staining. The approach enhances myelinated axons in rodent and human brain tissue, detects and quantifies white-matter injuries, and quantitatively characterizes white-matter microstructure. This non-destructive approach is compatible with subsequent complementary brain sample analysis by conventional histology and may become a novel gold-standard for investigating white-matter injury in the intact brain.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemistry & Molecular Biology
Estefania Martinez-Barrios, Sergi Cesar, Jose Cruzalegui, Clara Hernandez, Elena Arbelo, Victoria Fiol, Josep Brugada, Ramon Brugada, Oscar Campuzano, Georgia Sarquella-Brugada
Summary: Sudden death is rare in the pediatric population, and in cases where the cause is inconclusive, sudden arrhythmic death syndrome is suggested as the main potential cause. However, the current challenges in genetic diagnosis include establishing the correct genotype-phenotype association and classifying variants of uncertain significance.
Article
Health Care Sciences & Services
Estefania Martinez-Barrios, Georgia Sarquella-Brugada, Alexandra Perez-Serra, Anna Fernandez-Falgueras, Sergi Cesar, Monica Coll, Marta Puigmule, Anna Iglesias, Mireia Alcalde, Marta Vallverdu-Prats, Carles Ferrer-Costa, Bernat del Olmo, Ferran Pico, Laura Lopez, Victoria Fiol, Jose Cruzalegui, Clara Hernandez, Elena Arbelo, Simone Grassi, Antonio Oliva, Rocio Toro, Josep Brugada, Ramon Brugada, Oscar Campuzano
Summary: An exhaustive reanalysis of rare missense TTN variants classified before 2015 revealed that 36.8% were reclassified from unknown to benign/likely benign, while 38.3% showed a potential but not confirmed deleterious role. Most variants with a suspected deleterious role were identified in patients diagnosed with hypertrophic cardiomyopathy.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Genetics & Heredity
Mireia Alcalde, Laia Nogue-Novarro, Coloma Tiron, Anna Fernandez-Falgueras, Anna Iglesias, Adria Simon, Maria Buxo, Alexandra Perez-Serra, Marta Puigmule, Laura Lopez, Ferran Pico, Bernat del Olmo, Monica Corona, Oscar Campuzano, Sergio Moral, Josep Castella, Monica Coll, Ramon Brugada
Summary: Unexpected cardiac deaths pose a challenge to healthcare systems, with around 10%-15% remaining unexplained and potentially due to inherited arrhythmias. This study investigates the effect of non-benign genetic variants on heart measures and finds that they may modify certain measures in structurally normal hearts.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2022)
Correction
Genetics & Heredity
Julien Barc, Rafik Tadros, Charlotte Glinge, David Y. Chiang, Mariam Jouni, Floriane Simonet, Sean J. Jurgens, Manon Baudic, Michele Nicastro, Franck Potet, Joost A. Offerhaus, Roddy Walsh, Seung Hoan Choi, Arie O. Verkerk, Yuka Mizusawa, Soraya Anys, Damien Minois, Marine Arnaud, Josselin Duchateau, Yanushi D. Wijeyeratne, Alison Muir, Michael Papadakis, Silvia Castelletti, Margherita Torchio, Cristina Gil Ortuno, Javier Lacunza, Daniela F. Giachino, Natascia Cerrato, Raphael P. Martins, Oscar Campuzano, Sonia Van Dooren, Aurelie Thollet, Florence Kyndt, Andrea Mazzanti, Nicolas Clementy, Arnaud Bisson, Anniek Corveleyn, Birgit Stallmeyer, Sven Dittmann, Johan Saenen, Antoine Noel, Shohreh Honarbakhsh, Boris Rudic, Halim Marzak, Matthew K. Rowe, Claire Federspiel, Sophie Le Page, Leslie Placide, Antoine Milhem, Hector Barajas-Martinez, Britt-Maria Beckmann, Ingrid P. Krapels, Johannes Steinfurt, Bo Gregers Winkel, Reza Jabbari, Moore B. Shoemaker, Bas J. Boukens, Doris Skoric-Milosavljevic, Hennie Bikker, Federico Manevy, Peter Lichtner, Marta Ribases, Thomas Meitinger, Martina Muller-Nurasyid, Konstantin Strauch, Konstantin Strauch, Annette Peters, Holger Schulz, Lars Schwettmann, Reiner Leidl, Margit Heier, Jan H. Veldink, Leonard H. van den Berg, Philip Van Damme, Daniele Cusi, Chiara Lanzani, Sidwell Rigade, Eric Charpentier, Estelle Baron, Stephanie Bonnaud, Simon Lecointe, Audrey Donnart, Herve Le Marec, Stephanie Chatel, Matilde Karakachoff, Stephane Bezieau, Barry London, Jacob Tfelt-Hansen, Dan Roden, Katja E. Odening, Marina Cerrone, Larry A. Chinitz, Paul G. Volders, Maarten P. van de Berg, Gabriel Laurent, Laurence Faivre, Charles Antzelevitch, Stefan Kaab, Alain Al Arnaout, Jean-Marc Dupuis, Jean-Luc Pasquie, Olivier Billon, Jason D. Roberts, Laurence Jesel, Martin Borggrefe, Pier D. Lambiase, Jacques Mansourati, Bart Loeys, Antoine Leenhardt, Pascale Guicheney, Philippe Maury, Eric Schulze-Bahr, Tomas Robyns, Jeroen Breckpot, Dominique Babuty, Silvia G. Priori, Carlo Napolitano, Pascal Defaye, Pascal Defaye, Frederic Anselme, Jean Philippe Darmon, Francois Wiart, Carlo de Asmundis, Pedro Brugada, Ramon Brugada, Elena Arbelo, Josep Brugada, Philippe Mabo, Nathalie Behar, Carla Giustetto, Maria Sabater Molina, Juan R. Gimeno, Can Hasdemir, Peter J. Schwartz, Lia Crotti, Pascal P. McKeown, Sanjay Sharma, Elijah R. Behr, Michel Haissaguerre, Frederic Sacher, Caroline Rooryck, Hanno L. Tan, Carol A. Remme, Pieter G. Postema, Mario Delmar, Patrick T. Ellinor, Steven A. Lubitz, Jean-Baptiste Gourraud, Michael W. Tanck, Alfred L. George, Calum A. MacRae, Paul W. Burridge, Christian Dina, Vincent Probst, Arthur A. Wilde, Jean-Jacques Schott, Richard Redon, Connie R. Bezzina
Article
Multidisciplinary Sciences
Chloe Dumot, Chrystelle Po, Lucille Capin, Violaine Hubert, Elodie Ong, Matthieu Chourrout, Radu Bolbos, Camille Amaz, Celine Auxenfans, Emmanuelle Canet-Soulas, Claire Rome, Fabien Chauveau, Marlene Wiart
Summary: This study aimed to evaluate an intracerebral cell-based therapy for stroke in a preclinical setting. The observational study in rats with ischemic stroke found that side bias and axial diffusivity were the most relevant endpoints for evaluating treatment efficacy. The impact of lesion subtype and intracerebral administration on these endpoints was assessed. Sample size calculation indicated that a multicenter trial would be needed for a therapeutic trial based on these endpoints.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Marta Vallverdu-Prats, David Carreras, Guillermo J. J. Perez, Oscar Campuzano, Ramon Brugada, Mireia Alcalde
Summary: This study systematically describes gene and protein expression alterations in desmosomes, electrical conduction-related genes, and genes involved in fibrosis and adipogenesis in arrhythmogenic cardiomyopathy (ACM). The results show that loss of desmosomal genes leads to functional alterations in calcium handling, potentially contributing to the development of arrhythmogenic events in ACM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Marlene Wiart, Clement Tavakoli, Violaine Hubert, Ines Hristovska, Chloe Dumot, Stephane Parola, Frederic Lerouge, Fabien Chauveau, Emmanuelle Canet-Soulas, Olivier Pascual, David P. Cormode, Emmanuel Brun, Helene Elleaume
Summary: The activation of phagocytic cells is important in neurological diseases, and tracking them in the brain using MRI and CT can help understand their role and monitor therapeutic effects. Metal-based contrast agents like gadolinium, gold, and iron can be used for tracking phagocytic cells, but they may have toxicity and accumulation issues. Despite these challenges, early results in patients with neurological diseases are promising.
JOURNAL OF NEUROSCIENCE METHODS
(2023)
Article
Biotechnology & Applied Microbiology
Frederic Lerouge, Elodie Ong, Hugo Rositi, Francis Mpambani, Lise-Prune Berner, Radu Bolbos, Cecile Olivier, Francoise Peyrin, Vinu K. Apputukan, Cyrille Monnereau, Chantal Andraud, Frederic Chaput, Yves Berthezene, Bettina Braun, Mathias Jucker, Andreas K. O. Aslund, Sofie Nystrom, Per Hammarstrom, K. Peter Nilsson, Mikael Lindgren, Marlene Wiart, Fabien Chauveau, Stephane Parola
Summary: Aim: This study aimed to propose and evaluate a new multimodal imaging agent targeting amyloid-beta (A beta) plaques in Alzheimer's disease using various imaging techniques. Materials & methods: A new generation of hybrid contrast agents was designed and extensively characterized, and animal models of Alzheimer's disease were used for evaluation through MRI, two-photon microscopy, and synchrotron x-ray phase-contrast imaging. Results & conclusion: The study achieved two different grafting densities of luminescent-conjugated polythiophene while maintaining colloidal stability and fluorescent properties. In vivo brain uptake depended on the blood-brain barrier status. Nevertheless, successful A beta targeting was observed in both transgenic mice and A beta fibril-injected rats through multimodal imaging.
Article
Clinical Neurology
Laure Peter-Derex, Frederic Philippeau, Pierre Garnier, Nathalie Andre-Obadia, Sebastien Boulogne, Helene Catenoix, Philippe Convers, Laure Mazzola, Michel Gouttard, Maud Esteban, Julia Fontaine, Laura Mechtouff, Elodie Ong, Tae-Hee Cho, Norbert Nighoghossian, Nathalie Perreton, Anne Termoz, Julie Haesebaert, Anne-Marie Schott, Muriel Rabilloud, Christine Pivot, Carole Dhelens, Andrea Filip, Yves Berthezene, Sylvain Rheims, Florent Boutitie, Laurent Derex
Summary: This study found that prophylactic levetiracetam may reduce the risk of acute seizures in patients with intracerebral haemorrhage. However, larger studies are needed to determine whether seizure prophylaxis improves functional outcomes in these patients.
