Review
Biochemistry & Molecular Biology
Juan Garcia-Arriaza, Mariano Esteban, Daniel Lopez
Summary: There is a need for a highly effective vaccine against the emerging chikungunya virus, and this review discusses the development of potential vaccine candidates. It highlights the importance of understanding the immune responses induced by these candidates in animal models before progressing to human clinical trials.
Article
Medicine, General & Internal
Koen Bartholomeeusen, Matthieu Daniel, Desiree A. LaBeaud, Philippe Gasque, Rosanna W. Peeling, Kathryn E. Stephenson, Lisa F. P. Ng, Kevin K. Arien
Summary: Chikungunya virus is a widespread and recurring infectious disease that causes severe joint and muscle pain. It is transmitted by Aedes mosquitoes and has been causing outbreaks in various regions due to climate change and globalization. With no specific treatment or vaccines available, surveillance and vector control measures are crucial in preventing further re-emergence and epidemics.
NATURE REVIEWS DISEASE PRIMERS
(2023)
Article
Immunology
James M. Mccarty, Lisa Bedell, Jason Mendy, Emily E. Coates, Grace L. Chen, Julie E. Ledgerwood, Sarah Royalty Tredo, Kelly L. Warfield, Jason S. Richardson
Summary: This study analyzed the safety and immunogenicity of a chikungunya virus virus-like particle (CHIKV VLP) vaccine in individuals with different antibody statuses. The results showed that the CHIKV VLP vaccine was well-tolerated and immunogenic in individuals with pre-existing immunity.
Review
Immunology
Gerardo Montalvo Zurbia-Flores, Arturo Reyes-Sandoval, Young Chan Kim
Summary: Chikungunya virus (CHIKV) is a major global health threat, causing chikungunya fever (CHIKF) characterized by severe arthralgia. Despite its complex epidemiology and misrepresentation of its disease burden worldwide, there is still no licensed vaccine or antiviral treatment against CHIKV. This review highlights the clinical relevance of developing chikungunya vaccines, discussing the understanding of disease burden, epidemiological surveillance, and the global emergence of CHIKV infections, as well as recent progress in vaccine development.
Article
Immunology
Jingjing Liu, Xishan Lu, Xingxing Li, Weijin Huang, Enyue Fang, Wenjuan Li, Xiaohui Liu, Minglei Liu, Jia Li, Ming Li, Zelun Zhang, Haifeng Song, Bo Ying, Yuhua Li
Summary: This study developed an mRNA vaccine using the nucleotide sequence encoding structural proteins of CHIKV, and evaluated its immunogenicity. The encoded proteins elicited high levels of neutralizing antibody titers and T cell-mediated cellular immune responses in mice. Furthermore, a homologous booster mRNA vaccine regimen using different immunization strategies showed higher levels of neutralizing antibody titers and T-cell immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Sangwoo Nam, Yun Ji Ga, Joo-Youn Lee, Woo-Yeon Hwang, Eunhye Jung, Jin Soo Shin, Weiyi Chen, Gildon Choi, Bin Zhou, Jung-Yong Yeh, Yun Young Go
Summary: The study found that radicicol effectively suppressed CHIKV replication and may serve as a potential drug candidate against CHIKV. It also revealed nsP2 as a putative molecular target of radicicol, with the G641D mutation in nsP2 playing a key role in its interaction with cytoplasmic Hsp90 beta chaperone.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Virology
Thaise Yasmine Vasconcelos de Lima Cavalcanti, Mylena Ribeiro Pereira, Sergio Oliveira de Paula, Rafael Freitas de Oliveira Franca
Summary: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that has recently re-emerged, causing large-scale outbreaks worldwide. The infection mainly presents as severe joint pain and myalgia, but can also lead to severe complications such as neurological disorders and even death. Factors such as virus evolution, globalization, and climate change contribute to the spread of CHIKV, while the lack of preventive vaccines and effective antiviral treatments make it a major global health threat.
Article
Virology
Lady S. Lopez, Eliana P. Calvo, Jaime E. Castellanos
Summary: Ubiquitination and deubiquitination processes play important roles in regulating cellular protein function and stability. This study investigated the role of deubiquitinating enzymes (DUBs) in chikungunya virus (CHIKV) infection and found that inhibiting DUB activity impairs CHIKV replication by disrupting protein and viral RNA synthesis. Therefore, targeting DUB activity could be a potential strategy for blocking CHIKV infection.
Article
Immunology
Christin Schmidt, Florian D. Hastert, Julia Gerbeth, Tim Beissert, Ugur Sahin, Mario Perkovic, Barbara S. Schnierle
Summary: The study demonstrated that a CHIKV vaccine candidate based on trans-amplifying RNA can induce CHIKV- and RRV-specific immune responses in mice. However, higher antibody titers and neutralization capacity were observed after immunization with a single TR-RNA. There was no significant difference in alphavirus-specific T cell responses between bivalent and single vaccinations.
Article
Virology
Ya-Nan Zhang, Zhe-Rui Zhang, Na Li, Xin-Ru Pei, Xiao-Dan Li, Cheng-Lin Deng, Han-Qing Ye, Bo Zhang
Summary: This study successfully propagated Chikungunya virus in Vero cells through the construction of a chimeric VEEV-Delta C-CHIKV, significantly improving its yield. The passaged VEEV-Delta C-CHIKV demonstrated the safety and practicality of utilizing infectious capsidless viral particles of Chikungunya virus as a vaccine candidate.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Hammadul Hoque, Rahatul Islam, Srijon Ghosh, Md. Mashiur Rahaman, Nurnabi Azad Jewel, Md. Abunasar Miah
Summary: This study identified potential vaccine candidates for Chikungunya and Mayaro viruses based on immuno-informatics and computational methods, with multiple epitopes identified for CD8+ and CD4+ T cells as well as B cells. Analysis showed high population coverage globally, and molecular docking simulation demonstrated good HLA-epitope interaction for the predicted T cell epitopes. This research aims to pave the way for designing novel vaccines to combat both diseases simultaneously.
Article
Immunology
Awadalkareem Adam, Huanle Luo, Samantha R. Osman, Binbin Wang, Christopher M. Roundy, Albert J. Auguste, Kenneth S. Plante, Bi-Hung Peng, Saravanan Thangamani, Elena I. Frolova, Ilya Frolov, Scott C. Weaver, Tian Wang
Summary: The study demonstrates that the EILV/CHIKV vaccine candidate is safe, cost-effective to produce and a potent inducer of both innate and adaptive immunity in mice, showing good effects on CHIKV-specific antibodies and memory B cells, as well as CD8(+) T cell responses.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Virology
Liang Cao, Wei Wang, Wenchao Sun, Jinyong Zhang, Jicheng Han, Changzhan Xie, Zhuo Ha, Yubiao Xie, He Zhang, Ningyi Jin, Huijun Lu
Summary: This study investigated the use of adenovirus 5 as a vaccine vector for the development of a vaccine against Chikungunya virus (CHIKV). The results showed that the recombinant adenoviruses were able to elicit significant immune responses and provide protection against CHIKV infection in mice.
Article
Immunology
Jonathan O. Rayner, Jin Hyun Kim, Rosemary W. Roberts, Raphael Ryan Wood, Brian Fouty, Victor Solodushko
Summary: The study suggests that DNA launched VLP vaccines may have potential in preventing CHIKV infection compared to live attenuated vaccines, and gender differences in viral RNA loads in male and female mice were discovered, impacting the interpretation of vaccine efficacy and potentially influencing future CHIKV vaccine development.
Article
Immunology
Shambhavi Rao, Eranga Abeyratne, Joseph R. Freitas, Chenying Yang, Kothila Tharmarajah, Helen Mostafavi, Xiang Liu, Mehfuz Zaman, Suresh Mahalingam, Ali Zaid, Adam Taylor
Summary: Our study shows that using a gene delivery system could enhance the efficacy of live-attenuated vaccines encoded with a gene to prevent severe arthritic disease. The experiments conducted on mice demonstrate that this vaccine can provide long-lasting immune protection and has a systemic protective effect against CHIKV disease.
Article
Biology
Mohammad Uzzal Hossain, Arafat Rahman Oany, Shah Adil Ishtiyaq Ahmad, Md. Anayet Hasan, Md. Arif Khan, Md Al Ahad Siddikey
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2016)
Article
Genetics & Heredity
Md Anayet Hasan, Md Arif Khan, Tahmina Sharmin, Md Habibul Hasan Mazumder, Afrin Sultana Chowdhury
Article
Chemistry, Multidisciplinary
Mohammad Uzzal Hossein, Chaman Ara Keya, Keshob Chandra Das, Abu Hashem, Taimur Md Omar, Md Arif Khan, S. M. Rakib-Uz-Zaman, Md Salimullah
FRONTIERS IN CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Syed Shahariar Bappy, Sorna Sultana, Juthi Adhikari, Shafi Mahmud, Md. Arif Khan, K. M. Kaderi Kibria, Md. Masuder Rahman, Abu Zaffar Shibly
Summary: This study focused on designing a peptide-based epitope vaccine against CHIKV using immunoinformatics and docking tools. Two potential immunogenic envelope glycoprotein sequences were identified, interacting with HLA molecules with low IC50 scores and showing high conservancy in endemic countries. The identified peptides have great potential for vaccine development and further validation through antigen testing, production, and clinical trials is needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Md Arif Khan, Shafi Mahmud, A. S. M. Rubayet Ul Alam, Md Ekhtiar Rahman, Firoz Ahmed, Mohammed Rahmatullah
Summary: This study identified 23 potential drug candidates, among which Epirubicin, Vapreotida, and Saquinavir showed better binding affinity against SARS-CoV-2 Main Protease. Molecular dynamics simulation suggested the stability of the docked complex with these drugs. These findings suggest a potential inhibitory effect of these drugs on COVID-19 through synergistic interactions in the active cavity, paving the way for drug discovery with further in-vitro and in-vivo validations needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Shafi Mahmud, Md. Jahirul Islam, Md. Rimon Parves, Md. Arif Khan, Lamiya Tabussum, Sinthyia Ahmed, Md. Ackas Ali, Sayo O. Fakayode, Mohammad A. Halim
Summary: This study aimed to identify potent inhibitors of P-glycoprotein using computational approaches. Two compounds showed promising anti-tumor activity with acceptable pharmacokinetic properties, and molecular docking and dynamics simulations verified their stable binding with P-glycoprotein. Principal component analysis and QSAR modeling provided insights into the interactions and predictive capabilities of the drug candidates.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Chemistry, Multidisciplinary
Shafi Mahmud, Suvro Biswas, Gobindo Kumar Paul, Mohasana Akter Mita, Shamima Afrose, Md Robiul Hasan, Mst Sharmin Sultana Shimu, Mohammad Abu Raihan Uddin, Md Salah Uddin, Shahriar Zaman, K. M. Kaderi Kibria, Md Arif Khan, Talha Bin Emran, Md Abu Saleh
Summary: This study explored the potential of antiviral peptides in inhibiting the main protease of SARS-CoV-2, showing promising results in binding energy and stability of peptide-main protease complexes. Further wet-lab experiments are needed to confirm these findings.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Trina Ekawati Tallei, Fatimawali, Afriza Yelnetty, Rinaldi Idroes, Diah Kusumawaty, Talha Bin Emran, Talha Zahid Yesiloglu, Wolfgang Sippl, Shafi Mahmud, Taha Alqahtani, Ali M. Alqahtani, Saeed Asiri, Mohammed Rahmatullah, Rownak Jahan, Md Arif Khan, Ismail Celik
Summary: The article explores the potential of bromelain to counteract various SARS-CoV-2 variants by targeting its binding site with human ACE2. The research suggests that bromelain could serve as a promising inhibitor against different strains of the virus, although more studies are needed to verify its efficacy.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Infectious Diseases
A. M. U. B. Mahfuz, Md Arif Khan, Emran Hossain Sajib, Anamika Deb, Shafi Mahmud, Mahmudul Hasan, Otun Saha, Ariful Islam, Md Mizanur Rahaman
Summary: Nipah virus (NiV) is a zoonotic virus that causes severe infections in humans and animals. This study designed potential small interfering RNA (siRNA) molecules to silence the gene encoding the nucleocapsid protein of NiV, offering a promising molecular therapeutic approach against this dangerous virus.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Immunology
M. Nazmul Hoque, Md. Murshed Hasan Sarkar, Md. Arif Khan, Md. Arju Hossain, Md. Imran Hasan, Md. Habibur Rahman, Md. Ahashan Habib, Shahina Akter, Tanjina Akhtar Banu, Barna Goswami, Iffat Jahan, Tasnim Nafisa, Md. Maruf Ahmed Molla, Mahmoud E. Soliman, Yusha Araf, M. Salim Khan, Chunfu Zheng, Tofazzal Islam
Summary: This study utilized machine learning approaches to analyze RNA-seq data from COVID-19 patients, recovered individuals, and healthy individuals, finding disease-related differentially expressed genes. It provides insights into the role of SARS-CoV-2 infection in the pathophysiology and comorbidity of COVID-19.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Md. Arju Hossain, Sheikh Abdullah Al Ashik, Moshiur Rahman Mahin, Md. Al Amin, Md Habibur Rahman, Md. Arif Khan, Abdullah Al Emran
Summary: The study identified shared molecular etiology between PCOS and metabolic diseases through in-silico research, suggesting new therapeutic targets. Ten potential hub proteins were identified from protein-protein interaction analysis, with one significant hub protein, IGF2R, showing promising results in molecular docking studies.
Article
Multidisciplinary Sciences
Md Abdullah Al Mamun Khan, Asif Ahsan, Md Arif Khan, Jannatul Maowa Sanjana, Suvro Biswas, Md Abu Saleh, Dipali Rani Gupta, M. Nazmul Hoque, Tahsin Islam Sakif, Md Masuder Rahman, Tofazzal Islam
Summary: Magnaporthe oryzae causes destructive blast disease in major cereal crops and poses a threat to global food security. This study focused on discovering naturally occurring biofungicides as alternatives to synthetic chemical fungicides for controlling M. oryzae. Through molecular docking and simulation analysis, two alkaloidal metabolites, camptothecin and GKK1032A2, were identified as potential fungicides with excellent binding energy and bioactivity scores. These compounds showed superior efficacy compared to commercial fungicides. In particular, GKK1032A2 exhibited multi-site mode of action against M. oryzae. These findings suggest that camptothecin and GKK1032A2 could serve as lead compounds for developing novel fungicides against blast fungus.
Article
Chemistry, Multidisciplinary
A. M. U. B. Mahfuz, Md Arif Khan, Suvro Biswas, Shamima Afrose, Shafi Mahmud, Newaz Mohammed Bahadur, Firoz Ahmed
Summary: This study identified a potential pan-FGFR inhibitor from the PubChem database using a pharmacophore model generated from the structure of FGFR4 inhibited by LY2874455. Molecular docking and dynamics studies confirmed the initial finding, and ADMET analysis revealed similar properties between the identified compound and LY2874455. The compound has the potential to be used in the treatment of various cancers and can serve as a lead compound for the synthesis of more effective anticancer drugs.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
A. M. U. B. Mahfuz, Md Arif Khan, Promita Deb, Sharmin Jahan Ansary, Rownak Jahan
Summary: TBX5 gene encodes the transcription factor TBX5, crucial for heart and limb development. This study identified 5 most damaging SNPs through in silico analyses, predicting their structural and functional consequences using various tools and predictors. Wet-lab experiments can further validate these findings regarding their potential role in causing Holt-Oram Syndrome.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Toxicology
Amit Datta, Md. Jibran Alam, Laila Khaleda, Mohammad Al-Forkan
Summary: After arsenic exposure, rats supplemented with Corchorus olitorius and Butea monosperma showed relatively less severe toxicity effects, with significantly higher mtDNA copy numbers, reduced methylation levels in the p53 gene promoter, and decreased occurrence of mtDNA deletions. Histopathological analysis revealed less severe liver and kidney changes, with normal liver and kidney function parameters in supplemented rats. This indicates the protective properties of B. monosperma and C. olitorius against arsenic toxicity at a molecular level.
TOXICOLOGY REPORTS
(2021)