Article
Virology
Celine Chessa, Charles Bodet, Clement Jousselin, Andy Lariviere, Alexia Damour, Julien Garnier, Nicolas Leveque, Magali Garcia
Summary: LL-37 can directly inhibit West Nile virus (WNV), while hBD-3 does not have an inhibitory effect on WNV replication. Both peptides have immunomodulatory properties in WNV-infected keratinocytes.
Review
Biochemistry & Molecular Biology
Kornelius Zeth, Enea Sancho-Vaello
Summary: The human cathelicidin LL-37 is a versatile peptide with antimicrobial activity, playing a crucial role in targeting microorganisms. Recent structural studies have revealed the diversity and adaptability of LL-37 in interacting with lipid-like molecules. Complexes of LL-37 with detergents provide insights into mimicking lipids and inducing oligomerization and fibrillation, opening avenues for designing LL-37-derived peptides with enhanced antibiotic properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Kylen E. Ridyard, Mariam Elsawy, Destina Mattrasingh, Darien Klein, Janine Strehmel, Carole Beaulieu, Alex Wong, Joerg Overhage
Summary: The rise in antimicrobial resistant bacteria is limiting the number of effective treatments for bacterial infections. In this study, the synergy between the antimicrobial peptide LL-37 and various antibiotics was investigated against Escherichia coli and Pseudomonas aeruginosa strains. The combination of LL-37 and polymyxin B (PMB) showed strong synergy in preventing biofilm formation and eradicating pre-grown biofilms of E. coli and P. aeruginosa.
Article
Microbiology
Gladys Pinilla, Yenifer Tatiana Coronado, Gabriel Chaves, Liliana Munoz, Jeannette Navarrete, Luz Mary Salazar, Carlos Pelleschi Taborda, Julian E. Munoz
Summary: Fungal infections have become a major problem in immunosuppressed or ICU patients, and the resistance of fungi to conventional antifungal treatments is increasing. This study evaluated the in vitro antifungal activity of LL-37 analogue peptides against Candida spp. The results suggest that LL37-1 and AC-2 have the best inhibitory effect on Candida strains.
Article
Biochemical Research Methods
William F. Porto, Sergio A. Alencar
Summary: Cathelicidin antimicrobial peptides, including LL-37, are important host defense peptides with various activities. We investigated the effects of single nucleotide polymorphism (SNP)-driven point mutations on LL-37 structure and activity. Our results suggest that while the mutations had minimal impact on LL-37 activity, some mutations could reduce its antimicrobial activity and alter its structure.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2023)
Article
Microbiology
Yun-Qi Song, Su Min Kyung, Suji Kim, Gun Kim, So Yeong Lee, Han Sang Yoo
Summary: Pseudomonas aeruginosa is a major pathogen in nosocomial infections, which are difficult to handle due to its biofilm-forming ability and carbapenem resistance. Antimicrobial peptides, such as RP557, have shown effectiveness in inhibiting biofilms. Our study assessed the effectiveness of RP557 on carbapenem-resistant P. aeruginosa planktonic cells and biofilms, providing a potential treatment option for carbapenem-resistant P. aeruginosa infection. This treatment could be helpful in managing severe nosocomial infections caused by carbapenem-resistant bacteria and improving patients' survival rates.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Sara Masihzadeh, Mansour Amin, Zahra Farshadzadeh
Summary: This study investigated the antibiofilm activity of the WLBU2 peptide against Pseudomonas aeruginosa isolates. Results showed that WLBU2 exhibited strong inhibitory and eradication potency against MDR and carbapenem-resistant strains of P. aeruginosa, both in vitro and in vivo.
Article
Oncology
Qian Xiao, Yanfen Luo, Wen Shi, Yang Lu, Rui Xiong, Xinggui Wu, Haihao Huang, Chanjing Zhao, Jianming Zeng, Cha Chen
Summary: The antimicrobial peptide LL-37 regulates the quorum sensing system in Pseudomonas aeruginosa, inhibiting the expression of virulence factors and potentially serving as a candidate for QS inhibition and treatment of Pseudomonas aeruginosa biofilms.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Infectious Diseases
Malihe Mirzaee, Edita Holaskova, Alzbeta Micuchova, David J. Kopecny, Zhila Osmani, Ivo Frebort
Summary: This study demonstrates stable inheritance and expression of the LL-37 gene in barley over six generations, including two consecutive seasons of experimental field cultivation. The transgenic plants exhibited normal growth and remained fertile, with the produced LL-37 peptide retaining high antibacterial activity based on bacteria viability tests.
Article
Microbiology
Adam S. Bronson, Nina S. Baggett, Matthew T. Cabeen
Summary: Pyocins, interbacterial killing complexes produced by Pseudomonas aeruginosa, are primarily regulated by the activation of RecA due to DNA damage. However, our study reveals that the deletion of xerC enhances basal pyocin expression independently of RecA and that DNA damage-induced pyocin stimulation in Delta xerC cells is RecA-independent but dependent on PrtN. Moreover, inactivation of XerC recombinase activity leads to increased pyocin production, which is fully RecA dependent. These findings highlight the importance of XerC in regulating pyocin production and suggest potential strategies for targeting noncanonical pyocin production.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Roberto Vazquez, Antonio Domenech-Sanchez, Susana Ruiz, Julio Sempere, Jose Yuste, Sebastian Alberti, Pedro Garcia
Summary: Phage lysins are a potential alternative to antibiotics, especially for Gram-negative pathogens. In this study, a modified antimicrobial peptide derived from a phage lysin showed improved antibacterial activity, synergy with antibiotics, and low cytotoxicity.
Article
Immunology
Regina Geitani, Carole Ayoub Moubareck, Floriane Costes, Lea Marti, Gabrielle Dupuis, Dolla Karam Sarkis, Lhousseine Touqui
Summary: Cationic antimicrobial peptides (CAMPs) exert bactericidal effects against Pseudomonas aeruginosa (PA) depending on the timing of administration, with pretreatment of IB3-1 cells enhancing the bactericidal activity. LL-37 induces factors production in IB3-1 cells to improve bactericidal action.
MICROBES AND INFECTION
(2022)
Review
Infectious Diseases
Kylen E. Ridyard, Joerg Overhage
Summary: LL-37 shows promise as an alternative to conventional antibiotics due to its broad spectrum antibacterial and immunomodulatory properties, but faces limitations such as high cost and toxicity to human cells.
Article
Biochemistry & Molecular Biology
Paulina Piller, Heimo Wolinski, Robert A. Cordfunke, Jan Wouter Drijfhout, Sandro Keller, Karl Lohner, Nermina Malanovic
Summary: The development of antimicrobial agents against multidrug-resistant bacteria is crucial in the medical field. This study found that SAAP-148, an antimicrobial peptide, showed higher efficiency in killing bacteria and had stronger effects on membranes at both cellular and molecular levels compared to OP-145.
Article
Chemistry, Multidisciplinary
Bruno Casciaro, Maria Rosa Loffredo, Floriana Cappiello, Niamh O'Sullivan, Carola Tortora, Rizwan Manzer, Sougata Karmakar, Alan Haskell, Syed K. Hasan, Maria Luisa Mangoni
Summary: Antimicrobial resistance continues to be a problem, but a newly designed peptide called KDEON WK-11 shows strong antimicrobial activity against Pseudomonas aeruginosa with low cytotoxicity and potential anti-lipopolysaccharide qualities, making it a promising agent against sepsis.
FRONTIERS IN CHEMISTRY
(2022)
Article
Infectious Diseases
Rokeya Sultana Rekha, Akhirunnesa Mily, Tajnin Sultana, Ahsanul Haq, Sultan Ahmed, S. M. Mostafa Kamal, Annemarie van Schadewijk, Pieter S. Hiemstra, Gudmundur H. Gudmundsson, Birgitta Agerberth, Rubhana Raqib
BMC INFECTIOUS DISEASES
(2018)
Article
Multidisciplinary Sciences
Iwona T. Myszor, Zahida Parveen, Hakan Ottosson, Peter Bergman, Birgitta Agerberth, Roger Stromberg, Gudmundur H. Gudmundsson
SCIENTIFIC REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Zhiqian Yi, Yixi Su, Paulina Cherek, David R. Nelson, Jianping Lin, Ottar Rolfsson, Hua Wu, Kourosh Salehi-Ashtiani, Sigurdur Brynjolfsson, Weiqi Fu
MICROBIAL CELL FACTORIES
(2019)
Article
Immunology
Rokeya Sultana Rekha, Harpa Karadottir, Sultan Ahmed, Gudmundur H. Gudmundsson, Birgitta Agerberth, Peter Bergman
INFECTION AND IMMUNITY
(2020)
Review
Immunology
Peter Bergman, Rubhana Raqib, Rokeya Sultana Rekha, Birgitta Agerberth, Gudmundur H. Gudmundsson
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Immunology
Xinmeng Liao, Yawen Lan, Rui Shao, Jiayu Liu, Shufei Liang, Zhan Yin, Gudmundur H. Gudmundsson, Peter Bergman, Min Wan
Summary: Vitamin D has a significant impact on the generation and function of neutrophils in zebrafish, promoting granulopoiesis and enhancing neutrophil activity. VD also reduces bacterial counts and mortality in zebrafish infected with pathogens, in a neutrophil-dependent manner.
JOURNAL OF INNATE IMMUNITY
(2022)
Article
Immunology
Iwona T. Myszor, Snaevar Sigurdsson, Alexia Ros Viktorsdottir, Birgitta Agerberth, Eeva-Liisa Eskelinen, Margret Helga Ogmundsdottir, Gudmundur H. Gudmundsson
Summary: This study found that APD HO53 induces autophagy through a complex mechanism involving multiple pathways and epigenetic events. The induction of autophagy is associated with activation of AMPK, nuclear translocation of TFEB, and changes in expression of autophagy-related genes.
JOURNAL OF INNATE IMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Marjorie Laurence Barrier, Iwona Teresa Myszor, Priyanka Sahariah, Snaevar Sigurdsson, Miguel Carmena-Bargueno, Horacio Perez-Sanchez, Gudmundur Hrafn Gudmundsson
Summary: In the context of antibiotic resistance, the urgent need for alternative treatment strategies is addressed in this study. The researchers investigated the use of synthetized compounds called aroylated phenylenediamines (APDs) to induce the expression of a gene called cathelicidin antimicrobial peptide gene (CAMP) as a means to reduce the use of antibiotics during infection. The compound HO53 showed promising results in inducing CAMP expression in bronchial epithelium cells, and further analysis revealed its potential as a histone deacetylase (HDAC) inhibitor, which affects the acetylation status of cells and influences the expression of CAMP and other genes related to innate immunity and metabolism.
MOLECULAR IMMUNOLOGY
(2023)
Article
Orthopedics
Wen Deng, Zhiqian Yi, Enzhi Yin, Rui Lu, Hongbo You, Xuefeng Yuan
Summary: Supplementation of n-3 PUFAs is effective in relieving pain and improving joint function in patients with osteoarthritis (OA).
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Review
Immunology
Iwona T. Myszor, Gudmundur Hrafn Gudmundsson
Summary: The innate immune system of the airway epithelium consists of multiple components that work together to protect the host from invading pathogens. Modulating innate immune responses in the airway epithelium to boost host defenses and enhance immune responses is an alternative approach to standard antibiotics. This review discusses the possibilities of such modulation for host-directed therapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Xinmeng Liao, Yawen Lan, Wentao Wang, Jinjin Zhang, Rui Shao, Zhan Yin, Gudmundur H. Gudmundsson, Peter Bergman, Kangsen Mai, Qinghui Ai, Min Wan
Summary: This study used cyp2r1 mutant zebrafish and zebrafish fed a VD-deficient diet as VD-deficient animal models to investigate the role of VD in intestinal immunity against bacterial infection. The results showed that VD deficiency decreased the expression of antimicrobial peptides and IL-22, and increased susceptibility to bacterial infection in zebrafish. VD regulated gut microbiota composition and short-chain fatty acid production in the zebrafish intestine, leading to enhanced immunity.
Article
Medicine, Research & Experimental
Jon P. Joelsson, Iwona T. Myszor, Snaevar Sigurdsson, Fredrik Lehmann, Clive P. Page, Gudmundur H. Gudmundsson, Thorarinn Gudjonsson, Sigurbergur Karason
ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION
(2020)
Review
Infectious Diseases
Sultan Ahmed, Rubhana Raqib, Gudmundur Hrafn Gudmundsson, Peter Bergman, Birgitta Agerberth, Rokeya Sultana Rekha
Article
Medicine, Research & Experimental
Jon P. Joelsson, Iwona T. Myszor, Ari J. Arason, Saevar Ingthorsson, Paulina Cherek, Gabriel S. Windels, Kristjan Leosson, Gudmundur H. Gudmundsson, Thorarinn Gudjonsson, Sigurbergur Karason
ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION
(2019)
Article
Respiratory System
Ari Jon Arason, Jon Petur Joelsson, Bryndis Valdimarsdottir, Snaevar Sigurdsson, Alexander Gudjonsson, Skarphedinn Halldorsson, Freyr Johannsson, Ottar Rolfsson, Fredrik Lehmann, Saevar Ingthorsson, Paulina Cherek, Gudmundur H. Gudmundsson, Fridrik R. Gardarsson, Clive P. Page, Olafur Baldursson, Thorarinn Gudjonsson, Jennifer A. Kricker
RESPIRATORY RESEARCH
(2019)