Article
Cell & Tissue Engineering
Jiajia Xu, Yiyun Wang, Zhu Li, Ye Tian, Zhao Li, Amy Lu, Ching-Yun Hsu, Stefano Negri, Cammy Tang, Robert J. Tower, Carol Morris, Aaron W. James
Summary: Cells within the periosteum act as a stem cell reservoir for bone formation and repair. Activating PDGFR alpha reporter genes allows tracking and manipulation of these cells.
Article
Biochemistry & Molecular Biology
Ling-Ling Wang, Xiao-Yun Wan, Chun-Qi Liu, Fei-Meng Zheng
Summary: The study revealed a novel function of NDR1 in regulating BCSC properties by activating the Notch pathway, providing a potential strategy for eradicating BCSC to overcome tumor relapses, metastasis and drug resistance.
MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Mans Kadefors, Sara Rolandsson Enes, Emma Ahrman, Barbora Michalikova, Anna Lofdahl, Goran Dellgren, Stefan Scheding, Gunilla Westergren-Thorsson
Summary: Mesenchymal cells in the lung play crucial roles in tissue regeneration and repair, with dysregulation potentially leading to tissue remodeling in lung diseases. Two populations of CD105(+)CD90(+) mesenchymal cells, distinguished by their expression of CD13, show differences in clonogenic and proliferative capacity, with CD105(+)CD90(+)CD13(+) cells being more potent. Adventitial fibroblast subset is identified as the origin of these clonogenic mesenchymal cells in human lung through transcriptomic and spatial analysis.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
John P. Gleeson, Namit Chaudhary, Katherine C. Fein, Rose Doerfler, Patricia Hredzak-Showalter, Kathryn A. Whitehead
Summary: Breast milk contains cells that are beneficial for infant development, but little is known about these cells. This study characterized the cells in mature breast milk from healthy donors and identified the main cell population as epithelial cells, with several subpopulations including previously unidentified ones. These findings provide insights into the potential applications of breast milk cells in maternal and infant medicine.
Article
Cell Biology
Chen Gao, Xiaolan Qi, Xin Gao, Jin Li, Yumin Qin, Yunjun Yin, Fei Gao, Tao Feng, Sen Wu, Xuguang Du
Summary: Screening with a CRISPR library under stringent conditions revealed key regulators of pluripotency exit, including previously reported genes and 17 unreported genes. Zfp771 and Olfr769 were found to be potentially involved in pluripotency exit.
Article
Multidisciplinary Sciences
Rasmus Freter, Paola Falletta, Omid Omrani, Mahdi Rasa, Katharine Herbert, Francesco Annunziata, Alberto Minetti, Anna Krepelova, Lisa Adam, Sandra Kaeppel, Tina Ruediger, Zhao-Qi Wang, Colin R. Goding, Francesco Neri
Summary: The authors have developed a fluorescent Optical Stem Cell Activity Reporter (OSCAR) to assess RNA polymerase II activity and identify dormant cell populations in intestinal epithelial cells in vivo, providing a tool for a better understanding of dormant stem cell biology.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Abdel-Rahman N. Zekri, Abeer Bahnassy, Magda Mourad, Ibrahim Malash, Ola Ahmed, Mona S. Abdellateif
Summary: This study characterized breast cancer stem cells (BCSCs) through genetic profiling, revealing specific molecular profiles that differ according to their phenotypes. These profiles could potentially impact patients' prognosis and outcome.
CANCER CELL INTERNATIONAL
(2022)
Review
Medicine, Research & Experimental
Tianshu Zhang, Huimin Zhou, Kexin Wang, Xiaowei Wang, Mengyan Wang, Wenxia Zhao, Xiaoming Xi, Yang Li, Meilian Cai, Wuli Zhao, Yanni Xu, Rongguang Shao
Summary: Breast cancer is one of the most common malignant tumors in women and its occurrence has become the biggest threat. Understanding the characteristics and molecular mechanisms of breast cancer stem cells is crucial for developing targeted drugs.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Cell Biology
David van Bruggen, Fabio Pohl, Christoffer Mattsson Langseth, Petra Kukanja, Hower Lee, Alejandro Mossi Albiach, Mukund Kabbe, Mandy Meijer, Sten Linnarsson, Markus M. Hilscher, Mats Nilsson, Erik Sundstrom, Goncalo Castelo-Branco
Summary: The study identified the emergence of the first wave of oligodendrocyte lineage cells in the human forebrain as early as the 8th week of gestation, indicating evolutionary conservation of this process between mice and humans. Through analyzing the spatial expression of 50 key genes, regions in the human ventral fetal forebrain where oligodendrogenesis first occurs were identified.
DEVELOPMENTAL CELL
(2022)
Article
Cell & Tissue Engineering
Wei Shan, Qin Yu, Yan Long, Qian Luo, Honghu Li, Yingli Han, Yulin Xu, Shan Fu, Xiangjun Zeng, Cong Wei, Yang Gao, Xiaoqing Li, Xia Li, Lifei Zhang, Lizhen Liu, Ming Chen, Pengxu Qian, He Huang
Summary: This study established an in vitro differentiation system that successfully generated hematopoietic cells with in vivo hematopoietic reconstitution potential from mouse PSCs. The combination of 3D induction system and OP9 stromal cell coculture yielded c-kit(+) cells with superior repopulating activity.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Reza Mirzaei, Charlotte D'Mello, Marina Liu, Ana Nikolic, Mehul Kumar, Frank Visser, Pinaki Bose, Marco Gallo, V. Wee Yong
Summary: This study spatially characterized the heterogeneity of glioblastoma microenvironment and identified the potential therapeutic target of the biglycan-LRP6 axis in regulating tumor growth and brain tumor-initiating cells in GBM.
Article
Endocrinology & Metabolism
Jee-Eun Choi, Carlos Sebastian, Christina M. Ferrer, Caroline A. Lewis, Moshe Sade-Feldman, Thomas LaSalle, Anna Gonye, Begona G. C. Lopez, Walid M. Abdelmoula, Michael S. Regan, Murat Cetinbas, Gloria Pascual, Gregory R. Wojtkiewicz, Giorgia G. Silveira, Ruben Boon, Kenneth N. Ross, Itay Tirosh, Srinivas V. Saladi, Leif W. Ellisen, Ruslan I. Sadreyev, Salvador Aznar Benitah, Nathalie Y. R. Agar, Nir Hacohen, Raul Mostoslavsky
Summary: The study highlights the important tumor suppressor role of NAD(+)-dependent histone deacetylase sirtuin 6 in squamous cell carcinoma, as well as the existence of a subset of highly glycolytic tumor-propagating cells with elevated antioxidant capacity. These findings emphasize the key role of metabolism in intra-tumor heterogeneity.
Article
Biotechnology & Applied Microbiology
Carola Schmitz, Ekaterina Potekhina, Vsevolod V. Belousov, Antonina Lavrentieva
Summary: Cultivating human mesenchymal stem cells in a 3D environment is crucial for mimicking physiological conditions, improving therapeutic potential, and enhancing survival after implantation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Iva A. Tchasovnikarova, Sharon K. Marr, Manashree Damle, Robert E. Kingston
Summary: Researchers have developed a high-throughput technique called TRACE that allows the generation of fluorescent human reporter cell lines responsive to manipulation of epigenetic factors. This technique enables the genetic interrogation of various epigenetic pathways, contributing to a better understanding of chromatin biology.
Article
Multidisciplinary Sciences
Lai Man Natalie Wu, Feng Zhang, Rohit Rao, Mike Adam, Kai Pollard, Sara Szabo, Xuezhao Liu, Katie A. Belcher, Zaili Luo, Sean Ogurek, Colleen Reilly, Xin Zhou, Li Zhang, Joshua Rubin, Long-Sheng Chang, Mei Xin, Jiyang Yu, Mario Suva, Christine A. Pratilas, Steven Potter, Q. Richard Lu
Summary: Malignant peripheral nerve sheath tumor (MPNST) is a highly aggressive soft tissue sarcoma derived from benign neurofibroma (NF). A novel nestin-negative malignant stem-like subpopulation has been identified in MPNST, which is common to both mouse and human MPNSTs and correlates with clinical severity. Unique regulatory networks and druggable targets have been identified in MPNST, and targeting key epithelial-mesenchymal transition and stemness regulators can impede MPNST growth. These studies reveal the underlying principles of tumor cell-state evolution during NF-to-MPNST transformation and highlight a previously unrecognized mesenchymal stem-like subpopulation in MPNST disease progression.
News Item
Oncology
Frederic Catez, Nicole Dalla-Venezia, Jean-Jacques Diaz, Bertrand Dubois, Anthony Ferrari, Boris Guyot, Virginie Marcel, Maria Ouzounova, Roxane M. Pommier, Alain Viari, Patrick Mehlen
BULLETIN DU CANCER
(2020)
Article
Oncology
Allison E. Bridges, Sabarish Ramachandran, Rajneesh Pathania, Utkarsh Parwal, Adrienne Lester, Pragya Rajpurohit, Daley S. Morera, Nikhil Patel, Nagendra Singh, Hasan Korkaya, Santhakumar Manicassamy, Puttur D. Prasad, Vinata B. Lokeshwar, Bal L. Lokeshwar, Vadivel Ganapathy, Muthusamy Thangaraju
Article
Multidisciplinary Sciences
Roxane M. Pommier, Amelien Sanlaville, Laurie Tonon, Janice Kielbassa, Emilie Thomas, Anthony Ferrari, Anne-Sophie Sertier, Frederic Hollande, Pierre Martinez, Agnes Tissier, Anne-Pierre Morel, Maria Ouzounova, Alain Puisieux
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Maha Siouda, Audrey D. Dujardin, Laetitia Barbollat-Boutrand, Marco A. Mendoza-Parra, Benjamin Gibert, Maria Ouzounova, Jebrane Bouaoud, Laurie Tonon, Marie Robert, Jean-Philippe Foy, Vincent Lavergne, Serge N. Manie, Alain Viari, Alain Puisieux, Gabriel Ichim, Hinrich Gronemeyer, Pierre Saintigny, Peter Mulligan
Editorial Material
Cell Biology
Hasan Korkaya, Sandra Orsulic
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Oncology
Mohammad H. Rashid, Thaiz F. Borin, Roxan Ara, Raziye Piranlioglu, Bhagelu R. Achyut, Hasan Korkaya, Yutao Liu, Ali S. Arbab
Summary: MDSC-derived exosomes carry proteins that play a crucial role in tumor growth, invasion, angiogenesis, and immunomodulation. They are capable of hyper-activating or exhausting CD8 T-cells and inducing activation-induced cell death. In vivo experiments showed a depletion of CD8 T-cells and a reduction in pro-inflammatory M1-macrophages in the spleen after treatment with MDSC exosomes.
Article
Oncology
Allison E. Bridges, Sabarish Ramachandran, Kavin Tamizhmani, Utkarsh Parwal, Adrienne Lester, Pragya Rajpurohit, Daley S. Morera, Sarrah L. Hasanali, Pachiappan Arjunan, Ravirajsinh N. Jedeja, Nikhil Patel, Pamela M. Martin, Hasan Korkaya, Nagendra Singh, Santhakumar Manicassamy, Puttur D. Prasad, Vinata B. Lokeshwar, Bal L. Lokeshwar, Vadivel Ganapathy, Muthusamy Thangaraju
Summary: DNA repair processes have both protective and detrimental effects on colorectal cancer development. RAD51AP1 expression is significantly increased in colorectal cancer and may serve as a novel diagnostic marker and potential therapeutic target for the disease.
MOLECULAR CANCER RESEARCH
(2021)
Review
Oncology
Hadrien De Blander, Anne-Pierre Morel, Aruni P. Senaratne, Maria Ouzounova, Alain Puisieux
Summary: Cellular senescence is a form of cell cycle arrest induced by stresses like DNA damage and oncogenes, serving as a crucial barrier against cancer. However, senescent cells can evolve towards a pre-cancerous genotype and promote malignant phenotypes. Accumulated senescent cells not cleared by the immune system may secrete pro-inflammatory factors, further contributing to tumor development.
Meeting Abstract
Biochemistry & Molecular Biology
Elayne M. Benson, Megan A. Wilson, Emma V. Gray, Paris L. Rizzo, Maria Ouzounova, Hasan Korkaya, Austin Y. Shull
Editorial Material
Immunology
Anushka Dongre, Sandra Ortiz-Cuaran, Hasan Korkaya
FRONTIERS IN IMMUNOLOGY
(2022)
Meeting Abstract
Oncology
Alain Puisieux, Hadrien De Blander, Laurie Tonon, Frederique Fauvet, Roxane Pommier, Christelle Lamblot, Rahma Benhassoun, Benjamin Gibert, Maria Ouzounova, Anne-Pierre Morel
Article
Biochemistry & Molecular Biology
Evrin Gunebakan, Esra Yalcin, Esra Cikler Dulger, Ahmet Yigitbasi, Nilay Ates, Aysun Caglayan, Mustafa C. Beker, Kazim Sahin, Hasan Korkaya, Ertugrul Kilic
Meeting Abstract
Oncology
Emma V. Gray, Caroline E. Dyar, Maria Ouzounova, Max S. Wicha, Hasan Korkaya, Austin Y. Shull
Meeting Abstract
Oncology
Andrea Halaburkova, Vincent Cahais, Cyrille Cuenin, Rita Khoueiry, Maria Ouzounova, Akram Ghantous, Zdenko Herceg
Meeting Abstract
Oncology
Hasan Korkaya, Eunmi Lee, Raziye Piranioglu, Maria Ouzounova, Ahmet Korkaya, Jason Gestwicki, Max S. Wicha, Esteban Celis