Article
Cell Biology
Wenfei Pan, Limei Deng, Haitao Wang, Vivien Ya-Fan Wang
Summary: The NF-kappa B family of dimeric transcription factors regulate various biological functions. A study has revealed that the atypical I kappa B protein, Bcl3, plays a crucial role in enhancing the population of the p52:p52 homodimer within the NF-kappa B family. Bcl3 competes with other NF-kappa B subunits for efficient p52:p52 homodimer formation, leading to the upregulation of target genes involved in cell proliferation, migration, and inflammation. The aberrant activation of Bcl3 and p52 contributes to cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Giovanna M. Bernal, Longtao Wu, David J. Voce, Ralph R. Weichselbaum, Bakhtiar Yamini
Summary: This study found that the p52 subunit increases in the nucleus of cells during senescence, and factors in the conditioned media play a role in promoting p52 nuclear translocation. Additionally, mature p52 can induce cellular senescence. These findings support the observation of increased p52 in aged tissue and suggest that p52 contributes to organismal aging.
CELL AND BIOSCIENCE
(2022)
Review
Cell Biology
Gourisankar Ghosh, Vivien Ya-Fan Wang
Summary: The NF-kappa B family transcription factors, consisting of five monomers, play a critical role in regulating biological programs through DNA binding and transcriptional activation, despite their sequence and functional similarities, each member of the family displays distinct activities.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Nicholas Sim, Yinghui Li
Summary: p52-induced ETS1 overexpression in glioma cells remodels the genome-wide regulatory network of p52 and ETS1 to transcriptionally drive cancer progression. Chronic inflammation promotes glioma progression by activating NF-kappa B signalling, which promotes cancer cell invasion and angiogenesis. Non-canonical NF-kappa B activation directly regulates p52 at the ETS1 promoter, leading to its overexpression and impacting the genomic landscape of ETS1 in a glioma-specific manner.
COMMUNICATIONS BIOLOGY
(2023)
Article
Oncology
Thomas C. Case, Alyssa Merkel, Marisol Ramirez-Solano, Qi Liu, Julie A. Sterling, Renjie Jin
Summary: Management of CRPC remains challenging, with studies indicating the critical role of ARVs in resistance to AR inhibitors. Targeting GRP-R to block GRP/GRP-R signaling effectively inhibits NF-kappa B activity and ARVs expression, sensitizing CRPC cells to anti-androgen treatment and inhibiting tumor growth. Preclinical studies suggest combination therapy targeting both ARVs and AR-FL is a promising approach for CRPC and tNEPC.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Khrystany T. Isebia, Martijn P. Lolkema, Guido Jenster, Ronald de Wit, John W. M. Martens, Job van Riet
Summary: This study found that in metastatic castration-resistant prostate cancer, in addition to AR-V7, AR45 and AR-V3 were also commonly seen AR splice variants. The presence of any AR-Vs may be associated with higher AR expression. These AR-Vs could potentially serve as predictive and prognostic biomarkers in metastatic castration-resistant prostate cancer, similar or complementary to AR-V7.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Wenfei Pan, Vladimir A. Meshcheryakov, Tianjie Li, Yi Wang, Gourisankar Ghosh, Vivien Ya-Fan Wang, Volker Doetsch
Summary: The mammalian NF-kappa B p52:p52 homodimer, together with its cofactor Bcl3, activates transcription of kappa B sites with a central G/C base pair, while it is inactive towards kappa B sites with a central A/T base pair. The crystal structures and molecular dynamics simulations reveal that the conformation of DNA and binding kinetics play a crucial role in controlling transcriptional activation by NF-kappa B.
Article
Cell Biology
Dianbo Long, Yiyang Xu, Guping Mao, Ruobing Xin, Zengfa Deng, Hongyi Liao, Zhiwen Li, Zhi Yang, Baoxi Yu, Zhijian Yang, Aishan He, Ziji Zhang, Yan Kang
Summary: tRNA-derived fragments (tRFs) have been shown to play important roles in cell metabolism by regulating gene expression. This study investigates the role of tRFs in the cellular metabolism of the anterior cruciate ligament (ACL). The findings reveal that TRF365 regulates ACL cell metabolism by targeting IKBKB.
CELL DEATH DISCOVERY
(2022)
Article
Immunology
Yamel Cardona Gloria, Stephan H. Bernhart, Sven Fillinger, Olaf-Oliver Wolz, Sabine Dickhoefer, Jakob Admard, Stephan Ossowski, Sven Nahnsen, Reiner Siebert, Alexander N. R. Weber
Summary: The study reveals the lack of a negative feedback loop in B cells that restricts TLR signaling, leading to sustained NF-kappa B activation and lymphomagenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Yuri Tolkach, Anika Kremer, Gabor Lotz, Matthias Schmid, Thomas Mayr, Sarah Foerster, Stephan Garbe, Sana Hosni, Marcus Cronauer, Ildiko Kocsmar, Eva Kocsmar, Peter Riesz, Abdullah Alajati, Manuel Ritter, Joerg Ellinger, Carsten-Henning Ohlmann, Glen Kristiansen
Summary: The expression of androgen receptor splice variants, particularly AR-V7, in prostate cancer patients undergoing androgen deprivation therapy (ADT) may contribute to reduced therapy effects in patients receiving additional PARP inhibition due to the modulation of DNA repair gene expression. These findings suggest that AR splice variants could be further studied as predictive biomarkers for therapy response in this setting.
Article
Cell Biology
Tingting Zhou, Shengli Wang, Xiaoyu Song, Wensu Liu, Fang Dong, Yunlong Huo, Renlong Zou, Chunyu Wang, Siyi Zhang, Wei Liu, Ge Sun, Lin Lin, Kai Zeng, Xiang Dong, Qiqiang Guo, Fei Yi, Zhuo Wang, Xiaoman Li, Bo Jiang, Liu Cao, Yue Zhao
Summary: The study reveals the potential function of RNF8 in prostate cancer progression and its correlation with poor prognosis. RNF8 regulates prostate cancer development by altering histone modifications and promoting AR transcriptional activity and recruitment. RNF8 may serve as a potential therapeutic target for endocrine-resistant prostate cancer.
CELL DEATH & DISEASE
(2022)
Article
Biology
Sebastian Fritzwanker, Lionel Mouledous, Catherine Mollereau, Carine Froment, Odile Burlet-Schiltz, Felix Effah, Alexis Bailey, Mariana Spetea, Rainer K. Reinscheid, Stefan Schulz, Andrea Kliewer
Summary: Researchers developed a novel mouse model by fusing the hemagglutinin epitope tag sequence to the amino-terminus of the mu-opioid receptor, allowing for efficient immunodetection of G protein-coupled receptors. Mass spectrometry and immunodepletion experiments confirmed the efficacy of this approach for detecting low abundant GPCR targets.
COMMUNICATIONS BIOLOGY
(2021)
Article
Genetics & Heredity
Ilker Kiliccioglu, Cenk Y. Bilen, Sinan Sozen, Ece Konac
Summary: The study found that the expressions of AR, AR-V7, and AR-V567es were associated with the stage and Gleason Scores of prostate cancer in Met-PCa patients. Additionally, NF-K beta and HSP-27 protein levels were significantly higher in Met-PCa patients.
Article
Genetics & Heredity
Ilker Kiliccioglu, Cenk Y. Bilen, Sinan Sozen, Ece Konac
Summary: This study investigated the association of AR variants with inflammatory response and regulatory transcriptional activity genes in PCa and Met-PCa patients. The expression levels of AR, AR-V7 and AR-V567es were found to be correlated with prostate cancer stage and Gleason Scores in Met-PCa, while NF-Kβ and HSP-27 protein levels were significantly higher in Met-PCa patients. Targeting proteostasis and inflammation pathways by inhibiting HSP-27 and NF-Kβ could be a valuable strategy to overcome anti-androgen resistance and improve drug therapy in Met-PCa patients with high expression levels of AR-V7 and AR-V567es variants.
Article
Cell Biology
Enping Liu, Jiawei Sun, Jing Yang, Lin Li, Qili Yang, Jiuqin Zeng, Jiayu Zhang, Dahua Chen, Qinmiao Sun
Summary: ZDHHC11 positively regulates NF-kappa B signaling by promoting TRAF6 oligomerization and ligase activity, subsequently activating TAK1 and IKK complexes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)