Article
Biology
Shuchi Bhagi, Sudhir Chandna
Summary: Assessing alterations in the expression of radiation-responsive genes in peripheral blood cells is considered a promising approach for high-throughput radiation biodosimetry. The study found that incubating undiluted peripheral whole blood without any RNA stabilizing agent at 37°C for 24 hours resulted in the most optimal transcriptional response in the genes studied, with significant overexpression of DDB2 and FDXR.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Review
Biology
L. K. Wathen, P. S. Eder, G. Horwith, R. L. Wallace
Summary: Radiation biodosimetry is a continuously evolving clinical diagnostic field that uses biological markers to assess the amount of ionizing radiation absorbed. These biomarkers can help evaluate the likelihood of developing acute radiation syndrome.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Dorota Mlynarczyk, Pedro Puig, Carmen Armero, Virgilio Gomez-Rubio, Joan F. Barquinero, Monica Pujol-Canadell
Summary: This paper presents new Bayesian methods for dose estimation in radiation exposure using the gamma-H2AX biomarker, allowing for uncertainty in the time since exposure and producing more precise results. The Laplace approximation method is also utilized to reduce computation time.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Yaoxiang Li, Jatinder Singh, Rency Varghese, Yubo Zhang, Oluseyi O. Fatanmi, Amrita K. Cheema, Vijay K. Singh
Summary: The study used an NHP model to investigate the impact of gamma-irradiation on gene expression, and identified several signaling pathways associated with survival outcomes, which may help identify biomarkers for radiation injury.
SCIENTIFIC REPORTS
(2021)
Article
Biology
M. Abend, S. A. Amundson, C. Badie, K. Brzoska, R. Kriehuber, J. Lacombe, M. Lopez-Riego, K. Lumniczky, D. Endesfelder, G. O'Brien, S. Doucha-Senf, S. A. Ghandhi, R. Hargitai, E. Kis, L. Lundholm, D. Oskamp, P. Ostheim, S. Schuele, D. Schwanke, I. Shuryak, C. Siebenwith, M. Unverricht-Yeboah, A. Wojcik, J. Yang, F. Zenhausern, M. Port
Summary: This study conducted a gene expression analysis experiment in the context of the RENEB 2021 exercise and evaluated its performance for dose assessment. Three sets of samples were exposed to different X-ray doses and sent to eight teams for dose estimation and identification of relevant groups. The results showed that teams using a combination of genes achieved higher accuracy in dose estimation. Overall, this study demonstrated the potential of gene expression analysis in radiation events and preparedness for future emergency situations.
RADIATION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Alan Feiveson, Kerry George, Mark Shavers, Maria Moreno-Villanueva, Ye Zhang, Adriana Babiak-Vazquez, Brian Crucian, Edward Semones, Honglu Wu
Summary: The study investigates the impact of space radiation on astronauts' chromosomes and suggests that individual radiosensitivity may affect post-flight chromosome aberration rates. Additionally, both background CAR and radiosensitivity tend to increase with age.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Nanna Jorgensen, Anne-Vibeke Laenkholm, Susanne Gjorup Saekmose, Lone Bak Hansen, Thomas Vauvert F. Hviid
Summary: This study investigated systemic immune changes in ER-positive breast cancer patients and healthy controls, finding higher levels of neutrophils and monocytes, lower levels of lymphocytes and eosinophils in patients. CD25(+)CD127(low) Tregs were increased in patients and correlated with tumor size. Tumor grade also affected Treg numbers. The results suggest potential diagnostic and prognostic biomarkers in peripheral immune cell counts and Tregs.
CLINICAL IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Igor K. K. Khvostunov, Elena Nasonova, Valeriy Krylov, Andrei Rodichev, Tatiana Kochetova, Natalia Shepel, Olga Korovchuk, Polina Kutsalo, Petr Shegai, Andrei Kaprin
Summary: This study evaluates the cytogenetic damage induced by radioiodine therapy (RIT) in two rare differentiated thyroid cancer (DTC) cases, including the first follow-up study of a pediatric DTC patient. The results show that the risk of cytogenetic damage from RIT is low and a one-time administration of RIT is safe as long as the whole-body dose does not exceed 2 Gy. However, individual planning based on cytogenetic biodosimetry is strongly recommended for rare cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Genetics & Heredity
Vipul Vilas Wagh, Parin Vyas, Suchita Agrawal, Tejaswini A. Pachpor, Vasudeo Paralikar, Satyajeet P. Khare
Summary: Schizophrenia is characterized by delusions, hallucinations, and impaired socio-occupational function. Identifying biomarkers through blood-based gene expression studies can aid in early diagnosis and effective treatment strategies. The overlap of genetic, epigenetic, and gene expression changes suggests an interplay of genetic and environmental factors in schizophrenia.
FRONTIERS IN GENETICS
(2021)
Article
Oncology
Romain Banchereau, Avantika S. Chitre, Alexis Scherl, Thomas D. Wu, Namrata S. Patil, Patricia de Almeida, Edward E. Kadel, Shravan Madireddi, Amelia Au-Yeung, Chikara Takahashi, Ying-Jiun Chen, Zora Modrusan, Jacqueline McBride, Rhea Nersesian, Ehab A. El-Gabry, Mark D. Robida, Jeffrey C. Hung, Marcin Kowanetz, Wei Zou, Mark McCleland, Patrick Caplazi, Shadi Toghi Eshgi, Hartmut Koeppen, Priti S. Hegde, Ira Mellman, W. Rodney Mathews, Thomas Powles, Sanjeev Mariathasan, Jane Grogan, William E. O'Gorman
Summary: CD8+ tissue-resident memory T (T-RM) cells expressing CD103 (ITGAE) are believed to actively suppress cancer progression, and their presence in tumors may predict response to immunotherapy. This study found that in inflamed tumors, CD103+ CD8+ T-RM cells exhibit a complex phenotype characterized by increased clonal expansion, expression of checkpoint regulators, and cytotoxic proteins, suggesting their involvement in antitumor response.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Biology
Ekaterina Royba, Mikhail Repin, Adayabalam S. Balajee, Igor Shuryak, Sergey Pampou, Charles Karan, David J. Brenner, Guy Garty
Summary: The study presents the use of rhesus macaques as a model to validate the accuracy of the high-throughput version of the dicentric chromosome assay. Results indicate that rhesus macaques exhibit dose responses to gamma radiation similar to humans, with minor but statistically significant differences between the two model systems.
RADIATION RESEARCH
(2021)
Article
Spectroscopy
Pedro Arthur Augusto de Castro, Derly Augusto Dias, Matheus del-Valle, Marcelo Noronha Veloso, Elizabeth Sebastiana Ribeiro Somessari, Denise Maria Zezell
Summary: This study characterizes the bone dose response to ionizing radiation using bovine femurs, and the combination of FTIR spectroscopy and PLS-DA allows for detection of the effects of ionizing radiation on biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Przemyslaw J. Danek, Wladyslawa A. Daniel, Patrick M. Dansette
Summary: This study compares the effects of chronic treatment with Lurasidone on the expression of cytochrome P450 enzymes in rat liver and peripheral blood lymphocytes. It was found that Lurasidone inhibits the activity, protein and mRNA levels of CYP2B1/2, CYP2C11, and CYP2E1 in the liver, while inducing CYP3A1 and CYP3A2. In lymphocytes, Lurasidone decreases the expression of CYP2B, CYP2C11 (protein only), and CYP2E1, but increases CYP3A2 expression. These findings suggest that lymphocyte cytochrome P450 profile can be used as an indicator of hepatic cytochrome P450 profile in further clinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Ling-Hu Cai, Xiang-Yu Chen, Wei Qian, Chuan-Chuan Liu, Li-Jia Yuan, Liang Zhang, Chao Nie, Zhen Liu, Yue Li, Tian Li, Ming-Hua Liu
Summary: Through analyzing microarray datasets of human blood samples exposed to different types of radiation, 33 multi-type ray co-induced genes were identified, mainly enriched in DNA damage, cellular apoptosis, and p53 signaling pathway. 12 radiation-specific genes independent of trauma and burns were further screened. DDB2 gene performed the best in the diagnosis of all types of ray radiation, while MDM2 gene was the best indicator for assessing radiation dose.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Oncology
Petra Ross-Macdonald, Alice M. Walsh, Scott D. Chasalow, Ron Ammar, Simon Papillon-Cavanagh, Peter M. Szabo, Toni K. Choueiri, Mario Sznol, Megan Wind-Rotolo
Summary: Our study provides insights into the molecular characteristics of response and resistance to nivolumab in patients with metastatic ccRCC, potentially impacting patient selection and first-line treatment decisions. Treatment response is positively associated with T-cell infiltration but not with TCR clonality. Lower baseline T-cell infiltration is correlated with elevated transcription of Wnt/beta-catenin signaling components and hypoxia-regulated genes.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Environmental Sciences
Michael Abend, Daniela Stricklin, Nicole Flaig, Christophe Badie, Michel Drouet, Crawford Foster, Marek K. Janiak, Tjerk Kuipers, Florigio Lista, Ewa M. Nowosielska, Diane Riccobono, Stefania de Sanctis, Valeria Franchini, Ales Tichy, Matthias Port
Article
Oncology
B. Frey, J. Mika, K. Jelonek, L. Cruz-Garcia, C. Roelants, I Testard, N. Cherradi, K. Lumniczky, S. Polozov, A. Napieralska, P. Widlak, U. S. Gaipl, C. Badie, J. Polanska, S. M. Candelas
STRAHLENTHERAPIE UND ONKOLOGIE
(2020)
Article
Biochemistry & Molecular Biology
Lourdes Cruz-Garcia, Grainne O'Brien, Botond Sipos, Simon Mayes, Ales Tichy, Igor Sirak, Marie Davidkova, Marketa Markova, Daniel J. Turner, Christophe Badie
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Grainne O'Brien, Joanna Zyla, Kalliopi N. Manola, Maria N. Pagoni, Joanna Polanska, Christophe Badie
Summary: AML is an aggressive cancer with rapid progression, where cytogenetic and genetic analysis play crucial roles in diagnosis and prognosis. This study identified two novel deleterious mutations in DNMT3A and RUNX1 genes, providing additional information for patient diagnosis and prognosis. The exclusion and co-occurrence of specific gene mutation patterns in AML were also confirmed, offering insights into the mechanisms of AML leukaemogenesis.
LEUKEMIA & LYMPHOMA
(2021)
Article
Biology
Sjors Stouten, Sjoerd Verduyn Lunel, Rosemary Finnon, Christophe Badie, Fieke Dekkers
Summary: The study examined the impact of low-dose ionizing radiation on leukemia incidence, investigated the mechanisms of radiation-induced acute myeloid leukemia using an animal model, and performed simulations through a mathematical model. The findings indicated a proportional relationship between low-dose leukemia incidence and the number of cells with specific gene deletions, as well as discussed the impact of different data transformation methods on the results.
RADIATION AND ENVIRONMENTAL BIOPHYSICS
(2021)
Article
Biology
Farah Nasser, Lourdes Cruz-Garcia, Grainne O'Brien, Christophe Badie
Summary: Hypothermic conditions following irradiation significantly inhibited transcriptional responses to ionizing radiation exposure, but sample shipment at different temperatures did not affect gene expression level except for SESN1. The transcriptional response to IR of specific genes depended on the cell fraction used, while FDXR and CCNG1 showed consistent response across all conditions tested.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2021)
Editorial Material
Health Care Sciences & Services
Eric Andreas Rutten, Christophe Badie
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Orla Howe, Lisa White, Daniel Cullen, Grainne O'Brien, Laura Shields, Jane Bryant, Emma Noone, Shirley Bradshaw, Marie Finn, Mary Dunne, Aoife M. Shannon, John Armstrong, Brendan McClean, Aidan Meade, Christophe Badie, Fiona M. Lyng
Summary: Validation of a new 4-gene signature panel using G2 chromosomal radiosensitivity assay revealed significant differences in radiation response post-exposure, while some prostate cancer donors showed altered response due to Androgen Deprivation Therapy, suggesting intra-individual heterogeneity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biology
Patrick Ostheim, Sally A. Amundson, Christophe Badie, Dimitry Bazyka, Angela C. Evans, Shanaz A. Ghandhi, Maria Gomolka, Milagrosa Lopez Riego, Peter K. Rogan, Robert Terbrueggen, Gayle E. Woloschak, Frederic Zenhausern, Hanns L. Kaatsch, Simone Schule, Reinhard Ullmann, Matthias Port, Michael Abend
Summary: Radiation-induced gene expression changes can serve as early diagnostic and prognostic tools in nuclear or radiological events, helping differentiate exposure levels and predicting health effects, with the advantage of high-throughput and point-of-care diagnosis.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Melis Karabulutoglu, Rosemary Finnon, Lourdes Cruz-Garcia, Mark A. Hill, Christophe Badie
Summary: Haematopoietic bone marrow cells are highly sensitive to ionizing radiation, with potential risks for leukaemia development. This study investigates the effects of oxidative stress and X-ray exposure on murine haematopoietic stem and progenitor cells, revealing significant metabolic deregulation and mitochondrial dysfunctionality.
Article
Oncology
Lourdes Cruz-Garcia, Farah Nasser, Grainne O'Brien, Jakub Grepl, Volodymyr Vinnikov, Viktor Starenkiy, Sergiy Artiukh, Svetlana Gramatiuk, Christophe Badie
Summary: The FDXR gene was identified as the most sensitive and responsive to low dose radiation in cancer patients undergoing localized radiation treatment, showing a peak expression around 8-9 hours post-irradiation. The study also revealed the dynamic nature of DNA damage response in circulating blood leukocytes during radiotherapy, with FDXR expression correlating with the percentage of body exposed. Additionally, the upregulation of FDXR gene transcription was dose-dependent and could provide individual biological dosimetry information for predicting radiotherapy-associated adverse health outcomes.
Article
Biology
Sjors Stouten, Ben Balkenende, Lars Roobol, Sjoerd Verduyn Lunel, Christophe Badie, Fieke Dekkers
Summary: In vitro experiments have shown that cells responsible for radiation-induced acute myeloid leukemia (rAML) exhibit low-dose hyper-radiosensitivity (HRS). HRS leads to excess cell killing and the formation of chromosomal aberrations at low doses. However, little is known about the effect of HRS on low-dose cancer risk.
RADIATION AND ENVIRONMENTAL BIOPHYSICS
(2022)
Article
Biology
Dmitry Klokov, Kimberly Applegate, Christophe Badie, Dag Anders Brede, Fieke Dekkers, Melis Karabulutoglu, Yevgeniya Le, Eric Andreas Rutten, Katalin Lumniczky, Maria Gomolka
Summary: The concept of AOP has gained attention for its potential in incorporating mechanistic biological information in assessing adverse health outcomes following IR exposure. An international expert group proposed an AOP for IR-induced acute myeloid leukemia, highlighting the importance of further work in this area for the radiation protection research community.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ilona Barbara Csordas, Eric Andreas Rutten, Tuende Szatmari, Prabal Subedi, Lourdes Cruz-Garcia, David Kis, Balint Jezso, Christine von Toerne, Martina Forgacs, Geza Safrany, Soile Tapio, Christophe Badie, Katalin Lumniczky
Summary: Extracellular vesicles (EVs) play an important role in mediating bystander responses in irradiated bone marrow by altering cellular pathways through the miRNAs they carry. Using a mouse model, we analyzed the miRNA content of BM-derived EVs from mice irradiated with different doses and investigated the proteomic changes in BM cells upon direct irradiation or treatment with EVs. We identified key cellular processes regulated by miRNAs and suggested potential candidates involved in the regulation of these bystander processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Proceedings Paper
Computer Science, Interdisciplinary Applications
Justyna Mika, Serge M. Candeias, Christophe Badie, Joanna Polanska
Summary: This study aimed to investigate the feasibility of utilizing existing methods for TR sequence annotation in long-read sequencing data. TRUST4 algorithm detected the largest number of CDR3 sequences, MiXCR and TRUST4 algorithms showed the same distribution in annotated V and J genes, which can be used to analyze the repertoire of V/J genes used in rearranged TR genes.
BIOINFORMATICS AND BIOMEDICAL ENGINEERING, PT II
(2022)