Article
Multidisciplinary Sciences
Ruchi Kumari, Holger Hummerich, Xu Shen, Martin Fischer, Larisa Litovchick, Sibylle Mittnacht, James A. DeCaprio, Parmjit S. Jat
Summary: Cellular senescence is a stable cell cycle arrest that normal cells undergo after a finite number of divisions, largely established and maintained by the p53/p21(WAF1/CIP1) and pRB/p16(INK4A) tumour suppressor pathways. The DREAM complex plays a crucial role in senescence, with re-entry into the cell cycle bypassing senescence.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Qingfang Zheng, Ziyang Luo, Mingjun Xu, Shazhou Ye, Yuxin Lei, Yang Xi
Summary: High levels of HMGA1 and FOXM1 are associated with poor prognosis in various cancers, and their expression shows a significant positive correlation. HMGA1 and FOXM1 cooperate to up-regulate PLK1 and CCNB1, promoting cell cycle progression and cancer cell proliferation.
Article
Pharmacology & Pharmacy
Jinlian Wei, Xin Chen, Yongyun Li, Ruoxi Li, Keting Bao, Liang Liao, Yuqing Xie, Tiannuo Yang, Jin Zhu, Fei Mao, Shuaishuai Ni, Renbing Jia, Xiaofang Xu, Jian Li
Summary: In this study, a novel anti-conjunctival melanoma mechanism of cucurbitacin B (CuB) was discovered. CuB inhibited CM cell proliferation and induced G2/M cell cycle arrest. CuB targeted GRP78 and FOXM1-KIF20A pathway, providing a potential therapeutic strategy for CM.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Medicine, Research & Experimental
Eun-Taex Oh, Ha Gyeong Kim, Chul Hoon Kim, Jeonghun Lee, Chulhee Kim, Jae-Seon Lee, Yunmi Cho, Heon Joo Park
Summary: The overexpression of NQO1 is linked to tumor cell proliferation and growth in various human cancers. The molecular mechanisms underlying the role of NQO1 in cell cycle progression, specifically at the G2/M phase, are unclear. This study reveals a novel function of NQO1 in modulating CKS1, a cell cycle regulator, through its effect on the stability of c-Fos.
Article
Biochemistry & Molecular Biology
Erkut Ilaslan, Krystyna Kwiatkowska, Maciej Jerzy Smialek, Marcin Piotr Sajek, Zaneta Lemanska, Matisa Alla, Damian Mikolaj Janecki, Jadwiga Jaruzelska, Kamila Kusz-Zamelczyk
Summary: Nanos RNA-binding proteins play critical roles in germline development, and their dysfunction leads to infertility. The paralogues of Nanos proteins have divergent functions in germ cell biology, but the underlying molecular basis, such as their target mRNAs, remains unclear. In this study, RNA-sequencing analysis in a human primordial germ cell model revealed that Nanos1 and Nanos3 proteins influence different stages of the cell cycle. Additionally, Nanos3, in complex with RNA-binding protein PUM1, represses the expression of FOXM1 mRNA, a crucial transcription factor for the G2/M phase transition. The balance between FOXM1-NANOS3 and FOXM1-PUM1 expression levels is disrupted in testis cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Katarzyna Malarz, Jacek Mularski, Michal Kuczak, Anna Mrozek-Wilczkiewicz, Robert Musiol
Summary: Aromatic sulfonates based on quinazolines exhibit strong anticancer activity, showing high efficacy against a range of cancer cell lines with minimal impact on normal cell proliferation. They may serve as promising scaffolds for developing anticancer agents.
Article
Cell Biology
Tzu-Chien Lin, Ping-Jung Chung, Chen-An Shen, Thi My Hang Nguyenb, Yi-Syuan Lin, Shih-Chieh Lin, Shih-Chuan Hsiao, Wen-Tai Chiu
Summary: Intracellular calcium regulates the function of FOXM1, a transcription factor involved in cancer development. Depletion of calcium causes FOXM1 to aggregate on the nuclear envelope, and it is affected by the activity of exportin 1. Reduced calcium leads to increased SUMOylation of FOXM1, which is responsible for its sequestration.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shigeki Hirabayashi, Kotaro Shirakawa, Yoshihito Horisawa, Tadahiko Matsumoto, Hiroyuki Matsui, Hiroyuki Yamazaki, Anamaria Daniela Sarca, Yasuhiro Kazuma, Ryosuke Nomura, Yoshinobu Konishi, Suguru Takeuchi, Emani Stanford, Hideya Kawaji, Yasuhiro Murakawa, Akifumi Takaori-Kondo
Summary: A3B expression is regulated by cell cycle in both myeloma cells and normal blood cells, preferentially expressed in G2/M phase, and mainly found in plasma cells, CD10 positive B cells, and erythroid cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
Yuhang Bao, Xiuxian Wu, Xinghua Jin, Akihiro Kanematsu, Michio Nojima, Yoshiyuki Kakehi, Shingo Yamamoto
Summary: Apigenin has inhibitory effects on renal cell carcinoma (RCC), suppressing cell growth and inducing cell cycle arrest. It shows potential as a novel therapeutic strategy for RCC treatment due to its anticancer activity and ability to modulate the cell cycle.
Article
Biotechnology & Applied Microbiology
Shiqing Nie, Xin He, Zhiting Sun, Yan Zhang, Ting Liu, Tianfeng Chen, Jianfu Zhao
Summary: In this study, the anti-tumor effects of four different forms of selenium compounds combined with X-rays on SPC-A1 cells were investigated, and their inhibitory effects on cell proliferation and migration and their mechanisms were examined. It was found that the radiosensitizing effect of selenium on NSCLC was closely related to its selenium form.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Siobhan O'Brien, Susan Kelso, Zachary Steinhart, Stephen Orlicky, Monika Mis, Yunhye Kim, Sichun Lin, Frank Sicheri, Stephane Angers
Summary: This study reports a novel synthetic lethal genetic interaction between FBXW7 and CCNL1 and identifies CCNL1 as a substrate of the SCF-FBXW7 E3 ligase. Defective CCNL1 accumulation resulting from FBXW7 mutation leads to shorter mitotic time. Cells with FBXW7 loss-of-function mutations are highly sensitive to treatment with a CDK11 inhibitor, suggesting a potential genetic vulnerability for cancer treatment.
Article
Biochemistry & Molecular Biology
Yazhini Velappan, Ambra de Simone, Santiago Signorelli, John A. Considine, Christine H. Foyer, Michael J. Considine
Summary: Hydrogen cyanamide (HC) is commonly used in horticulture to promote bud burst after dormancy, however, its use is banned in some countries due to health concerns. The mechanism of HC action is not well understood, hindering the search for safe alternatives. This study investigated the effects of HC on reactive oxygen species (ROS), redox homeostasis, and cell division in Arabidopsis thaliana seedlings. HC treatment resulted in reversible inhibition of root growth and an accumulation of cells in the G2/M phase accompanied by increased cellular oxidation.
Article
Chemistry, Medicinal
Tai-Hsin Tsai, Cheng-Yu Tsai, Sin-Hua Moi, Chieh-Hsin Wu, Kuan-Ting Lee, Yi-Chiang Hsu, Yu-Feng Su
Summary: CDDO-dhTFEA has anticancer effects on glioblastoma cells by reducing cell proliferation and regulating cell cycle proteins. It induces G2/M cell cycle arrest and inhibits proliferation in U87MG and GBM8401 cells.
Article
Medicine, Research & Experimental
Yu-gang Huang, Dan Li, Li Wang, Xiao-min Su, Xian-bin Tang
Summary: The study revealed that CENPF is significantly overexpressed in ACC patients, which is positively correlated with tumor stage and associated with unfavorable overall survival in ACC patients. GSEA analysis suggested that CENPF is mainly involved in regulating the G2/M phase of the cell cycle and the p53 signaling pathway. Experimental findings indicated that the interaction between CENPF and CDK1 facilitates the G2/M phase transition, cell proliferation, and potentially induces a p53-mediated anti-tumor effect in ACC. Additionally, immune infiltration analysis highlighted the potential use of CENPF inhibitors like Cisplatin, Sunitinib, and Etoposide for ACC therapy.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, Research & Experimental
Sanchari Dasgupta, Kanisha Kar, Atish Barua, Diya Ghosh, Bikash Kabi, Koushik Dewan, Arpita Chandra
Summary: A novel cobalt complex 1 exhibits significant anticancer activity with lower toxicity compared to oxaliplatin against MCF-7 cells, and shows no significant toxicity to the host. This complex has the potential to be a new chemotherapeutic agent for anti-tumor medication.
Letter
Oncology
Patrick Casey, Yeng Ang, Javed Sultan
Summary: The long-term physiological consequences of SARS-CoV-2 infection are not well understood, and COVID-19 may lead to chronic illness, sarcopenia, and physical deconditioning, potentially exacerbating frailty in patients. Further research is needed to explore the mechanisms behind the deconditioning effects of SARS-CoV-2 infection and how this impacts treatment of malignant disease.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2021)
Review
Cell Biology
Ying Ye, Xi Chen, Wensheng Zhang
Summary: Embryonic stem cells have a unique ability to maintain and regulate the balance between self-renewal and multi-lineage cellular differentiation, largely dependent on gene expression regulations. Chromatin remodeling complexes play a crucial role in promoting chromatin structural changes that ultimately affect ESC fate choices.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Wei Xu, Yi Wen, Yingying Liang, Qiushi Xu, Xuefei Wang, Wenfei Jin, Xi Chen
Summary: This protocol describes a fast and robust method for single-cell chromatin accessibility profiling using ATAC-seq, combining bulk tagging and flow cytometry. It provides critical information about cell type composition and variability within complex tissues. The experimental procedure can be completed within 1 or 2 days, generating high complexity data with excellent signal-to-noise ratio.
Article
Immunology
Xuefei Wang, Xiangru Shen, Shan Chen, Hongyi Liu, Ni Hong, Hanbing Zhong, Xi Chen, Wenfei Jin
Summary: Classic T cell subsets are defined by cell surface markers, while single-cell RNA sequencing clusters cells based on gene expression profiles. Multiple T cell subsets can be assigned to one cluster with similar expression patterns. Higher levels of ISAGs may contribute to quick immune responses.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Elizabeth Ratcliffe, James Britton, Shaheen Hamdy, John McLaughlin, Yeng Ang
Summary: This review explores the emerging role of dedicated services for patients with Barrett's oesophagus (BO). The research indicates that dedicated services can improve guideline adherence and increase dysplasia detection rates. Additionally, patients report high levels of cancer worry and poor disease-specific knowledge.
BMJ OPEN GASTROENTEROLOGY
(2022)
Article
Cell Biology
Jack L. McMurray, Anouk von Borstel, Taher E. Taher, Eleni Syrimi, Graham S. Taylor, Maria Sharif, Jamie Rossjohn, Ester B. M. Remmerswaal, Frederike J. Bemelman, Felipe A. Vieira Braga, Xi Chen, Sarah A. Teichmann, Fiyaz Mohammed, Andrea A. Berry, Kirsten E. Lyke, Kim C. Williamson, Michael J. T. Stubbington, Martin S. Davey, Carrie R. Willcox, Benjamin E. Willcox
Summary: Gamma delta T cells are traditionally considered innate-like lymphocytes, but a new adaptive-like compartment has now been identified. This adaptive subset undergoes pathogen-driven differentiation, similar to conventional CD8(+) T cells. Expansions of specific TCRs are detected exclusively in the effector cells, indicating a specialized response to antigens.
Article
Biology
Alvin Wei Tian Ng, Gianmarco Contino, Sarah Killcoyne, Ginny Devonshire, Ray Hsu, Sujath Abbas, Jing Su, Aisling M. Redmond, Jamie M. J. Weaver, Matthew D. Eldridge, Simon Tavare, Paul A. W. Edwards, Rebecca C. Fitzgerald
Summary: This study categorizes genomic structural variants (SVs) in 383 cases of oesophageal cancer, uncovering mutational patterns and new disease drivers. Identification of SV events in OAC is crucial for targeted therapies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Surgery
Ajith K. Siriwardena
Summary: This study provides a global overview of the management of patients with acute cholecystitis during the early phase of the COVID-19 pandemic. The pandemic led to a decrease in surgical workforce and operating facilities, worse severity of disease, and increased use of conservative management. While the number of patients undergoing cholecystectomy decreased, there was no increase in post-operative mortality.
Review
Geriatrics & Gerontology
Patrick Casey, Mohamed Alasmar, John McLaughlin, Yeng Ang, Jamie McPhee, Priam Heire, Javed Sultan
Summary: Ultrasound is a promising tool for measuring skeletal muscle mass and quality, and can predict functional capacity, clinical outcomes, and survival. However, further standardization is needed before ultrasound can be widely used in nutritionally vulnerable patients.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Pathology
Maja Kopczynska, Elizabeth Ratcliffe, Harika Yalamanchili, Anna Thompson, Adib Nimri, James Britton, Yeng Ang
Summary: IDD in Barrett's esophagus carries a significant risk of progression to dysplasia or neoplasia, highlighting the importance of accurate histological reporting for diagnosis and management.
JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Biochemical Research Methods
Wei Xu, Weilong Yang, Yunlong Zhang, Yawen Chen, Ni Hong, Qian Zhang, Xuefei Wang, Yukun Hu, Kun Song, Wenfei Jin, Xi Chen
Summary: ISSAAC-seq is a highly sensitive and flexible single-cell multi-omics method that allows for joint profiling of chromatin accessibility and gene expression. It provides critical information about cell types and cell states, revealing heterogeneity at the chromatin level within cell types defined by gene expression.
Article
Biology
Georgia Tsagkogeorga, Helena Santos-Rosa, Andrej Alendar, Dan Leggate, Oliver Rausch, Tony Kouzarides, Hendrik Weisser, Namshik Han
Summary: The study uses machine learning on multi-modal data to predict novel genes and molecular pathways involved in RNA methylation in humans.
COMMUNICATIONS BIOLOGY
(2022)
Article
Genetics & Heredity
Danling Zhu, Yi Wen, Wanyue Yao, Haiyan Zheng, Sixian Zhou, Qiqi Zhang, Li-Jia Qu, Xi Chen, Zhe Wu
Summary: Epigenetic reprogramming in plant germline cells involves widespread chromatin bivalency and restricted resetting of H3K27me3 at developmental regulators. The incorporation of histone variant H3.10 helps establish the unique chromatin identity of sperm without impacting somatic H3K27me3 resetting. Additionally, specific H3K27me3 domains are present at repressed genes in vegetative nuclei, while pollination-related genes are highly expressed and marked by gene body H3K4me3.
Article
Oncology
Nancy Alnassar, Malgorzata Borczyk, Georgia Tsagkogeorga, Michal Korostynski, Namshik Han, Dariusz C. Gorecki
Summary: Mutations in the DMD gene cause Duchenne muscular dystrophy (DMD), but recent research shows that reduced DMD expression is also associated with various malignancies. The downregulation of DMD in tumors is correlated with reduced patient survival and tumor stage. These findings challenge the current understanding of dystrophin expression and suggest a broader significance of the DMD gene beyond DMD.
Article
Biochemistry & Molecular Biology
Deyue Yang, Fengli Zhao, Danling Zhu, Xi Chen, Xiangxiong Kong, Yufeng Wu, Min Chen, Jiamu Du, Li-Jia Qu, Zhe Wu
Summary: The RNA-binding protein RZ-1 and the polycomb complex 2 (PRC2) work together to silence ABA biosynthesis genes and ensure seed germination.