Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome

Specific RNA Recognition by Designer Pentatricopeptide Repeat Protein

(2015) Cuicui Shen et al.   Molecular Plant

A universal strategy for regulating mRNA translation in prokaryotic and eukaryotic cells

(2015) Jicong Cao et al.   NUCLEIC ACIDS RESEARCH

Bidirectional Regulation of mRNA Translation in Mammalian Cells by Using PUF Domains

(2014) Jicong Cao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Pentatricopeptide Repeat Proteins in Plants

(2014) Alice Barkan et al.   Annual Review of Plant Biology

Programmable RNA recognition and cleavage by CRISPR/Cas9

(2014) Mitchell R. O’Connell et al.   NATURE

Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity

(2014) John P Guilinger et al.   NATURE METHODS

A protein-RNA specificity code enables targeted activation of an endogenous human transcript

(2014) Zachary T Campbell et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization

(2014) Chen Qiu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

An artificial PPR scaffold for programmable RNA recognition

(2014) Sandrine Coquille et al.   Nature Communications

A Novel Function of Human Pumilio Proteins in Cytoplasmic Sensing of Viral Infection

(2014) Ryo Narita et al.   PLoS Pathogens

Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA

(2014) Zhanar Abil et al.   Journal of Biological Engineering

Engineering RNA-binding proteins for biology

(2013) Yu Chen et al.   FEBS Journal

Engineered proteins with Pumilio/fem-3mRNA binding factor scaffold to manipulate RNA metabolism

(2013) Yang Wang et al.   FEBS Journal

Treatment of Type 1 Myotonic Dystrophy by Engineering Site-specific RNA Endonucleases that Target (CUG) n Repeats

(2013) Wenjing Zhang et al.   MOLECULAR THERAPY

Structural basis for the modular recognition of single-stranded RNA by PPR proteins

(2013) Ping Yin et al.   NATURE

Structural basis for RNA recognition by a dimeric PPR-protein complex

(2013) Jiyuan Ke et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Elucidation of the RNA Recognition Code for Pentatricopeptide Repeat Proteins Involved in Organelle RNA Editing in Plants

(2013) Yusuke Yagi et al.   PLoS One

Improved Computational Target Site Prediction for Pentatricopeptide Repeat RNA Editing Factors

(2013) Mizuki Takenaka et al.   PLoS One

Competition and collaboration between RNA-binding proteins and microRNAs

(2013) J. J. David Ho et al.   Wiley Interdisciplinary Reviews-RNA

Mechanistic Insight into Pentatricopeptide Repeat Proteins as Sequence-Specific RNA-Binding Proteins for Organellar RNAs in Plants

(2012) T. Nakamura et al.   PLANT AND CELL PHYSIOLOGY

Mitochondrial ribonuclease P structure provides insight into the evolution of catalytic strategies for precursor-tRNA 5' processing

(2012) M. J. Howard et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Engineering RNA endonucleases with customized sequence specificities

(2012) Rajarshi Choudhury et al.   Nature Communications

A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins

(2012) Alice Barkan et al.   PLoS Genetics

Visualization of Nonengineered Single mRNAs in Living Cells Using Genetically Encoded Fluorescent Probes

(2011) Toshimichi Yamada et al.   ANALYTICAL CHEMISTRY

Deciphering the role of RNA-binding proteins in the post-transcriptional control of gene expression

(2011) S. Kishore et al.   Briefings in Functional Genomics

Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains

(2011) Shuyun Dong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Divergence of Pumilio/fem-3mRNA Binding Factor (PUF) Protein Specificity through Variations in an RNA-binding Pocket

(2011) Chen Qiu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of human mitochondrial RNA polymerase

(2011) Rieke Ringel et al.   NATURE

A universal code for RNA recognition by PUF proteins

(2011) Aleksandra Filipovska et al.   Nature Chemical Biology

The prospects for designer single-stranded RNA-binding proteins

(2011) Joel P Mackay et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Assembly of custom TALE-type DNA binding domains by modular cloning

(2011) Robert Morbitzer et al.   NUCLEIC ACIDS RESEARCH

Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

(2011) Ting Li et al.   NUCLEIC ACIDS RESEARCH

Assembly of Designer TAL Effectors by Golden Gate Cloning

(2011) Ernst Weber et al.   PLoS One

Selection patterns on restorer-like genes reveal a conflict between nuclear and mitochondrial genomes throughout angiosperm evolution

(2011) S. Fujii et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted translational regulation using the PUF protein family scaffold

(2011) A. Cooke et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Designer RNA-binding proteins: New tools for manipulating the transcriptome

(2011) Aleksandra Filipovska et al.   RNA Biology

Alternate Modes of Cognate RNA Recognition by Human PUMILIO Proteins

(2011) Gang Lu et al.   STRUCTURE

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

PUF proteins: repression, activation and mRNA localization

(2010) Tara Quenault et al.   TRENDS IN CELL BIOLOGY

Roles of Puf proteins in mRNA degradation and translation

(2010) Melanie A. Miller et al.   Wiley Interdisciplinary Reviews-RNA

Understanding and engineering RNA sequence specificity of PUF proteins

(2009) Gang Lu et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structure and RNA binding of the mouse Pumilio-2 Puf domain

(2009) Huw T. Jenkins et al.   JOURNAL OF STRUCTURAL BIOLOGY

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Engineering splicing factors with designed specificities

(2009) Yang Wang et al.   NATURE METHODS

Small silencing RNAs: an expanding universe

(2009) Megha Ghildiyal et al.   NATURE REVIEWS GENETICS

A 5' cytosine binding pocket in Puf3p specifies regulation of mitochondrial mRNAs

(2009) D. Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein

(2009) Y. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A single C. elegans PUF protein binds RNA in multiple modes

(2009) Y. Y. Koh et al.   RNA

Structure, dynamics, and function of RNA modification enzymes

(2008) Ryuichiro Ishitani et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Ribonomic Analysis of Human Pum1 Reveals cis-trans Conservation across Species despite Evolution of Diverse mRNA Target Sets

(2008) A. R. Morris et al.   MOLECULAR AND CELLULAR BIOLOGY

Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p

(2008) Matthew T Miller et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Live-cell imaging of viral RNA genomes using a Pumilio-based reporter

(2008) Jens Tilsner et al.   PLANT JOURNAL

Comparative Analysis of mRNA Targets for Human PUF-Family Proteins Suggests Extensive Interaction with the miRNA Regulatory System

(2008) Alessia Galgano et al.   PLoS One

Structures of Human Pumilio with Noncognate RNAs Reveal Molecular Mechanisms for Binding Promiscuity

(2008) Yogesh K. Gupta et al.   STRUCTURE