Article
Biochemistry & Molecular Biology
Yutong Wang, Xu Yan, Mei Xu, Weiyang Qi, Chunjie Shi, Xiaohong Li, Jiaqi Ma, Dan Tian, Jianxin Shou, Haijun Wu, Jianwei Pan, Bo Li, Chao Wang
Summary: Clathrin-mediated endocytosis (CME) is a major endocytic pathway in eukaryotic cells that regulates the abundance of plasma membrane proteins. The phytohormone auxin differentially regulates the clathrin heavy chains (CHCs) and light chains (CLCs), as well as the distribution of the auxin efflux transporter PIN2. The involvement of transmembrane kinase (TMKs) family proteins, specifically TMK1, in the regulation of clathrin and CME by auxin has been discovered, highlighting their critical role in establishing an asymmetric distribution of PIN2 and an auxin gradient during root gravitropism.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Claudio Osorio-Navarro, Jorge Toledo, Lorena Norambuena
Summary: Sucrose induces cell elongation and endocytosis in plant roots, and its regulation is reversible. The endocytosis mediated by sucrose is dependent on clathrin-mediated mechanism and plays a role in sensing energy status and signaling root elongation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Xidian Tang, Yanfei Xie, Guanhua Li, Zhannur Niyazbekova, Shaofei Li, Jianjun Chang, Dekun Chen, Wentao Ma
Summary: The entry of ORFV into target cells is mediated by clathrin-mediated endocytosis and macropinocytosis, while caveolae-dependent endocytosis has minor effects. Additionally, ORFV entry into cells requires an acidic environment and the involvement of dynamin.
VETERINARY MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soshiro Kodera, Toshihide Kimura, Tomoki Nishioka, Yukiko K. Kaneko, Momoka Yamaguchi, Kozo Kaibuchi, Tomohisa Ishikawa
Summary: Clathrin-dependent endocytosis is a crucial process for secretory cells and disruption of this process can lead to various diseases. This study reveals the role of GDP-bound Rab27a and its interacting protein HSPA8 in regulating different stages of glucose-induced endocytosis in pancreatic β-cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Multidisciplinary Sciences
Alexander Johnson, Dana A. Dahhan, Nataliia Gnyliukh, Walter A. Kaufmann, Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, Monika Hrtyan, Jie Wang, Juan Aguilera-Servin, Daniel Van Damme, Emmanuel Beaurepaire, Martin Loose, Sebastian Y. Bednarek, Jiri Friml
Summary: This study identified the TPLATE complex as a key component in the unique plant endocytosis mechanism, playing a crucial role in mediating membrane bending against high-turgor pressure in plant cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Shikha T. Ramesh, Kolaparamba V. Navyasree, Sneha Sah, Anjitha B. Ashok, Nishada Qathoon, Suryasikha Mohanty, Rajeeb K. Swain, Perunthottathu K. Umasankar
Summary: This study uncovers the role of an uncharacterized kinase BMP2K in AP-2 phosphorylation, demonstrating that BMP2K interacts with AP-2 via its C-terminus, and this interaction is crucial for CCP localization and function. The decline of endogenous BMP2K levels upon AP-2 impairment suggests a stabilizing relationship, and functional inactivation of BMP2K in zebrafish embryos leads to gastrulation phenotypes similar to AP-2 loss-of-function, highlighting the physiological relevance of BMP2K in vertebrates.
Article
Biology
Tania Lopez-Hernandez, Koh-Ichiro Takenaka, Yasunori Mori, Pornparn Kongpracha, Shushi Nagamori, Volker Haucke, Shigeo Takamori
Summary: This study found that the endocytosis of synaptic vesicles in mouse hippocampal neurons at physiological temperature is independent of clathrin, while the endocytic retrieval of certain synaptic vesicle proteins, including vesicular transporters for glutamate and GABA, relies on sorting by the clathrin adaptor AP-2.
Article
Cell Biology
Ying Zhang, Jing Zhu, Hao Xu, Qin Yi, Liang Yan, Liang Ye, Xinyuan Zhang, Min Xie, Bin Tan
Summary: Using real-time confocal microscopy and structured illumination microscopy, we observed the time-dependent internalization of fluorescently labeled S100B protein (S100B-Alexa488) into MSCs and its transport through endolysosomal pathways. Inhibitors and RNA interference approaches further demonstrated that clathrin and lipid rafts play important roles in the internalization of S100B-Alexa488, providing potential interventions for the safe application of MSCs in glioma therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Pallavi V. Vetal, Yves Poirier
Summary: Inorganic phosphate (Pi) homeostasis is crucial for plant growth and relies on the transport of Pi across plant cells. A protein called PHO1 plays a role in this transport process, but its localization on the plasma membrane does not align with its function. By inhibiting a process called clathrin-mediated endocytosis (CME), researchers discovered that PHO1 can be re-localized to the plasma membrane, which affects its transport function.
Article
Microbiology
Wei Pan, Nie Hui, Hongmei Wang, Hongbin He
Summary: Bovine parainfluenza virus 3 enters MDBK cells via macropinocytosis and clathrin-mediated endocytosis, requiring acid-dependent entry and cathepsin L. Additionally, macropinocytosis is dependent on actin dynamics during BPIV3 infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zheng Wu, Chengyu Fan, Yi Man, Yue Zhang, Ruili Li, Xiaojuan Li, Yanping Jing
Summary: The study demonstrated that clathrin-mediated endocytosis and membrane microdomain-associated endocytosis are both involved in the adaptation to hyperosmotic stress in plant cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Zhongzheng Ma, Yang Zheng, Zijian Chao, Hongtao Chen, Yunhui Zhang, Meizhen Yin, Jie Shen, Shuo Yan
Summary: A star polymer with positively-charged tertiary amine was synthesized and used to investigate the mechanism of gene delivery. The star polymer could protect gene fragments from degradation and promote cellular uptake and endocytosis. Transcriptome analysis revealed up-regulation of key genes involved in endocytosis. This study provides the first direct visualization of the detailed cellular process and mechanism of nanocarrier-mediated gene delivery.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Plant Sciences
Lihui Jiang, Baolin Yao, Xiaoyan Zhang, Lixia Wu, Qijing Fu, Yiting Zhao, Yuxin Cao, Ruomeng Zhu, Xinqi Lu, Wuying Huang, Jianping Zhao, Kuixiu Li, Shuanglu Zhao, Li Han, Xuan Zhou, Chongyu Luo, Haiyan Zhu, Jing Yang, Huichuan Huang, Zhengge Zhu, Xiahong He, Jiri Friml, Zhongkai Zhang, Changning Liu, Yunlong Du
Summary: Salicylic acid inhibits rice root growth by interfering with auxin transport through OsPIN3t-mediated mutation and clathrin-mediated endocytosis, which is different from TyrA23-mediated root growth.
Article
Immunology
Ruihan Shi, Lei Hou, Li Wei, Jue Liu
Summary: Clathrin-mediated endocytosis serves as the initial step for effective viral infection by allowing viruses to enter cells. Adaptor proteins play a crucial role in this process, with their research shedding light on the viral life cycle and paving the way for novel therapeutics.
MICROBIAL PATHOGENESIS
(2021)
Article
Veterinary Sciences
Ngo Thi Phuong Lan, Lizhen Wang, Wei Li, Xiaolan Hou, Wenchi Chang, Bo Wen, Shuizhong Han, Yan Chen, Xuefeng Qi, Jingyu Wang
Summary: The entry mechanism of fowl adenovirus serotype-4 (FAdV-4) into liver cells was investigated. The results showed that FAdV-4 entered cells through clathrin-mediated endocytosis dependent on cholesterol and macropinocytosis requiring PI3K. These findings provide important insights into the entry mechanisms of other avian adenoviruses.
VETERINARY RESEARCH
(2023)