Review
Chemistry, Medicinal
Farooqahmed S. Kittur, Chiu-Yueh Hung, P. Andy Li, David C. Sane, Jiahua Xie
Summary: Neuroprotective drugs for cerebral ischemia and reperfusion (I/R) injury are urgently needed, but the clinical translation of recombinant human erythropoietin (rhuEPO(M)) has been inconsistent due to side effects. Asialo-rhuEPO, a non-erythropoietic derivative, has shown neuroprotective effects and can be prepared by removing sialic acid residues. Both types of asialo-rhuEPO showed excellent neuroprotective effects in animal models, and future studies are needed to develop it as a multimodal neuroprotectant for ischemic stroke treatment.
Article
Neurosciences
Sijie Li, Wenbo Zhao, Guiyou Liu, Changhong Ren, Ran Meng, Yuan Wang, Haiqing Song, Qingfeng Ma, Yuchuan Ding, Xunming Ji
Summary: Chronic remote ischemic conditioning (RIC) appears to be well-tolerated and associated with a low annual risk of ischemic stroke and cardio-cerebrovascular events in patients with symptomatic internal carotid artery or middle cerebral artery occlusion with impaired hemodynamics.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Review
Neurosciences
Lang Zeng, Shengqi Hu, Lingcheng Zeng, Rudong Chen, Hua Li, Jiasheng Yu, Hongkuan Yang
Summary: Ischemic stroke is a common type of stroke that has a significant impact on human well-being and quality of life. To better understand the pathophysiology of ischemic stroke and develop new treatment strategies, controllable and consistent animal models are urgently needed. The middle cerebral artery occlusion (MCAO) model is currently the most widely used model for studying ischemic stroke. This review discusses different methods for constructing the MCAO model and compares their advantages and disadvantages, aiming to provide better approaches for studying ischemic stroke.
Article
Neurosciences
Michael Bieber, Michael K. Schuhmann, Alexander M. Kollikowski, David Stegner, Bernhard Nieswandt, Mirko Pham, Guido Stoll
Summary: Research has shown that blocking the activatory platelet receptor can delay the progression of neocortical infarction in acute ischemic stroke caused by large vessel occlusion, as well as mitigate the local vascular recruitment of neutrophils and T-cells. This experimental data supports ongoing clinical trials of blocking platelet GPVI in acute ischemic stroke.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Immunology
Uma Maheswari Selvaraj, Thomas A. Ujas, Xiangmei Kong, Ashwani Kumar, Erik J. Plautz, Shanrong Zhang, Chao Xing, Tiffany L. Sudduth, Donna M. Wilcock, Jadwiga Turchan-Cholewo, Mark P. Goldberg, Ann M. Stowe
Summary: The study shows that CD8 T cells persist in the ischemic brain and have a long-term impact on functional recovery. Depletion of CD8 T cells can improve motor recovery, reduce leukocyte infiltration, as well as decrease the numbers of macrophages, neutrophils, and CD4 T cells in the injured brain.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Dunpeng Cai, Mikayla Fraunfelder, Ken Fujise, Shi-You Chen
Summary: Astrocytes play a critical role in stroke outcomes. This study reveals that ADAR1, an RNA editing enzyme, is highly induced in astrocytes in ischemic stroke lesions, and its deficiency improves stroke outcomes by reducing activated astrocytes and microglia. ADAR1 promotes astrocyte proliferation and induces neuron apoptosis through secretion of inflammatory cytokines.
Article
Clinical Neurology
Dongze Guo, Xing Fan, Hao You, Xiaorong Tao, Lei Qi, Miao Ling, Zhibao Li, Jiajia Liu, Hui Qiao
Summary: This study revealed a significant association between the duration of MEP changes and postoperative motor dysfunction in patients with middle cerebral artery aneurysms. It also proposed a critical cutoff value of 8.5 minutes for predicting the risk of postoperative motor dysfunction.
NEUROSURGICAL REVIEW
(2021)
Article
Cell Biology
Bei-Yao Gao, Yi-Xing Cao, Peng-Fei Fu, Ying Xing, Dan Liang, Shan Jiang, Yu-Xiao Xie, Min Li
Summary: This study investigated the role of optogenetic cALM stimulation in a mouse model of cerebral stroke and found that 21-day optogenetic cALM inhibition improved neurological function, while activation did not. Furthermore, stimulation and inhibition had different effects on neural plasticity.
NEURAL REGENERATION RESEARCH
(2022)
Review
Clinical Neurology
Raymond C. Koehler, Valina L. Dawson, Ted M. Dawson
Summary: Parthanatos is a cell death signaling pathway caused by excessive oxidative damage to DNA, potentially playing a role in cerebral artery occlusion in young male rodents. PARP inhibitors may have neuroprotective effects and could be repurposed for stroke, although further research is needed to evaluate their efficacy in aged animals or those with comorbidities.
FRONTIERS IN NEUROLOGY
(2021)
Review
Cell Biology
Kitti Thiankhaw, Nipon Chattipakorn, Siripron C. Chattipakorn
Summary: MCAO and cerebral I/R injury can lead to microvascular injury, blood-brain barrier dysfunction, inflammation, oxidative stress, and neuronal death, resulting in brain infarction. HBOT has shown positive effects on neurological outcomes after these injuries, but conflicting data exists on its impact on functional recovery. Studies have been conducted to evaluate the potential benefits of HBOT, both in vivo and clinically, in the context of stroke outcomes.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biology
Sui-yi Xu, Mao-mei Song, Xue Pan, Shi-na Song, Qi Zhang, Chang-xin Li
Summary: A modified technique for sampling the ischemic border zone in a rat model of cerebral ischemia has been proposed in this study. The technique, based on modifications of existing methods, provides a directly observable infarct borderline and a sufficient sampling area. The reliability and reproducibility of the technique were verified through the expression of selected biomarkers.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Shirley H. T. Truong, Benjamin Bonnici, Samoda Rupasinghe, Barbara K. Kemp-Harper, Chrishan S. Samuel, Brad R. S. Broughton
Summary: Brain inflammation and apoptosis contribute to neuronal damage and loss following ischaemic stroke, leading to cognitive and functional disability. H2-relaxin, a hormone with anti-inflammatory and anti-apoptotic effects, shows potential as a therapeutic for stroke. This study investigated the effects of post-stroke administration of H2-relaxin and found that it improved motor function and decreased apoptosis in a dose-dependent manner.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Neurosciences
Xiuping Chen, Wansong Wang, Hui Li, Xin Zhang
Summary: Enriched environment (EE) can protect against cerebral ischemia/reperfusion (I/R) injury in rats by modulating the expression of gap junction protein Connexin 43 (Cx43) and suppressing inflammation and oxidative stress. This finding sheds light on the protective mechanism of EE.
Article
Clinical Neurology
Miao Chen, Feng Wang, Hairong Wang
Summary: The long noncoding RNA XLOC_035088 plays a crucial role in ischemic stroke by protecting the brain against ischemic injury and improving neurological function, providing a promising therapeutic route for the treatment of ischemic stroke.
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2021)
Article
Clinical Neurology
Maotao He, Farooqahmed S. Kittur, Chiu-Yueh Hung, Jianhui Zhang, Li Jing, David C. Sane, P. Andy Li, Jiahua Xie
Summary: The study demonstrates that non-sialylated rhuEPO(P) is a non-erythropoietic and non-immunogenic neuroprotectant in a cerebral ischemia and reperfusion mouse model, showing significant reduction in cerebral infarct volume and edema, as well as improvement in neurological deficits and histopathological outcome. Non-sialylated rhuEPO(P) displays pleiotropic neuroprotective effects, similar to rhuEPO(M), making it a promising candidate for stroke treatment.
TRANSLATIONAL STROKE RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Kentaro Kogure, Tatsuya Fukuta
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
(2020)
Review
Pharmacology & Pharmacy
Mahadi Hasan, Anowara Khatun, Tatsuya Fukuta, Kentaro Kogure
ADVANCED DRUG DELIVERY REVIEWS
(2020)
Article
Pharmacology & Pharmacy
Tatsuya Fukuta, Shota Hirai, Tatsusada Yoshida, Takashi Maoka, Kentaro Kogure
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2020)
Article
Chemistry, Multidisciplinary
Tatsuya Fukuta, Yasufumi Oshima, Kohki Michiue, Daichi Tanaka, Kentaro Kogure
JOURNAL OF CONTROLLED RELEASE
(2020)
Review
Pharmacology & Pharmacy
Tatsuya Fukuta, Kentaro Kogure
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
(2020)
Article
Biochemistry & Molecular Biology
Yosuke Yanagida, Mio Namba, Tatsuya Fukuta, Hirokazu Yamamoto, Mitsuaki Yanagida, Miki Honda, Naoto Oku, Tomohiro Asai
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Pharmacology & Pharmacy
Dai Majima, Ryosuke Mitsuhashi, Misaki Yamasaki, Kazuaki Kajimoto, Tatsuya Fukuta, Kentaro Kogure
Summary: The study demonstrated that α-tocopheryl succinate (TS) can suppress lipid accumulation in adipocytes by regulating lipid metabolic cell signaling, promoting lipolysis, and inhibiting lipid synthesis.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Chemistry, Medicinal
Tatsuya Fukuta, Shintaro Yoshimi, Kentaro Kogure
Summary: The study showed that leukocyte-mimetic liposomes containing leukocyte membrane proteins can effectively penetrate into tumor spheroids, inhibit tumor growth, and potentially serve as a drug delivery system for cancer treatment. Transferring leukocyte membrane proteins onto liposomes enhances their binding capacity with tumor cells, demonstrating the ability for efficient penetration into tumors.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Chemistry, Organic
Atsushi Nakayama, Tenta Nakamura, Tabassum Ara, Tatsuya Fukuta, Sangita Karanjit, Takeshi Harada, Asuka Oda, Hideo Sato, Masahiro Abe, Kentaro Kogure, Kosuke Namba
Summary: In this study, a biaryl dimer based on eurotiumide A was synthesized, showing potent antioxidant activities without cytotoxicity against murine colon and human myeloma cells.
TETRAHEDRON LETTERS
(2021)
Article
Pharmacology & Pharmacy
Tatsuya Fukuta, Daichi Tanaka, Shinya Inoue, Kohki Michiue, Kentaro Kogure
Summary: Combining tight junction-opening peptide AT1002 with iontophoresis (IP) can overcome the thickened pathological skin in psoriasis, facilitate macromolecule delivery, and enable intradermal delivery of NF-xB decoy oligodeoxynucleotide for psoriasis treatment.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Pharmacology & Pharmacy
Tatsuya Fukuta, Naoto Oku, Kentaro Kogure
Summary: Ischemic stroke is a major cause of high mortality and severe disability. Nanoparticles, including liposomes, provide a promising solution for drug delivery across the blood-brain barrier. Biomimetic drug delivery systems using cellular membranes/membrane proteins are also being investigated.
Article
Biochemistry & Molecular Biology
Shintaro Yoneda, Tatsuya Fukuta, Mizune Ozono, Kentaro Kogure
Summary: Delivery of cerebroprotective agents using liposomes has shown potential for treating cerebral ischemia/reperfusion (I/R) injury. In a rat model, lipid nanoparticles (LNP) with particle sizes <100 nm exhibited better therapeutic effects and significantly improved brain injury compared to larger LNP. These findings suggest that regulating the particle size of liposomes can enhance the therapeutic effect of encapsulated drugs for cerebral I/R injury.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Tatsuya Fukuta, Mayumi Ikeda-Imafuku, Satoshi Kodama, Junko Kuse, Ko Matsui, Yasunori Iwao
Summary: High-pressure homogenization (HPH) using a microfluidizer can efficiently prepare PEG-modified exosomes encapsulating anti-cancer drugs, which shows potential for cancer treatment.
Article
Chemistry, Multidisciplinary
Hiroyuki Koide, Chiaki Kiyokawa, Anna Okishima, Kaito Saito, Keiichi Yoshimatsu, Tatsuya Fukuta, Yu Hoshino, Tomohiro Asai, Yuri Nishimura, Yoshiko Miura, Naoto Oku, Kenneth J. Shea
Summary: This article describes a synthetic antibody that binds to HMGB1 and inhibits its interaction with the RAGE receptor, thereby reducing brain damage caused by cerebral ischemia/reperfusion.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Pharmacology & Pharmacy
Tatsuya Fukuta, Natsu Nakatani, Shintaro Yoneda, Kentaro Kogure
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2020)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)