期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 422, 期 1-2, 页码 332-337出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2011.11.001
关键词
Bubble liposomes; Ultrasound; Gene delivery; Tongue tissue
资金
- New Energy, the Industrial Technology Development Organization (NEDO) of Japan [04A05010]
Oral squamous cell carcinoma is the most common type of head and neck cancer. Recently, efficient, easy, and minimally invasive gene delivery methods are expected to be developed as cancer gene therapies. However, the optimal method for delivering therapeutic genes into oral tissue for cancer treatment has not been elucidated. Therefore, we hypothesized that the tongue is a good target tissue for gene delivery with Bubble liposomes and ultrasound. To assess this, we attempted to deliver a mixture of plasmid DNA encoding a luciferase or enhanced green fluorescent protein, and Bubble liposomes into murine tongue with or without ultrasound exposure. The ultrasound conditions were 1 MHz, 2 W/cm(2), 60 s, and duty cycle: 50%. The time-course of gene expression in the tongue was investigated with a luciferase assay and fluorescent microscopy. Luciferase expression was significantly increased in tongue transfected using Bubble liposomes and ultrasound compared with that of the tongue untreated with ultrasound, and this high level of luciferase activity was maintained for 2 weeks. From these results, Bubble liposomes can be used in combination with ultrasound to efficiently deliver plasmid DNA into the tongue in vivo. This technique is a highly promising approach for gene delivery into oral tissue. (c) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据