Article
Chemistry, Physical
Daniel Stengel, Betuel Hilal Demirel, Patrick Knoll, Martyna Truszkowska, Flavia Laffleur, Andreas Bernkop-Schnuerch
Summary: This study evaluated the impact of polyethylene glycol (PEG) and zwitterionic surface decoration of lipid-based nanocarriers (NC) on cellular uptake. The results showed that zwitterionic lipid-based NCs exhibited better performance in terms of particle size, stability, and cellular uptake compared to PEGylated NCs. The replacement of PEG surfactants with zwitterionic surfactants may be a promising approach for intracellular drug delivery.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Yuyun Tang, Jinchao Gao, Tao Wang, Qian Zhang, Antian Wang, Meng Huang, Renhe Yu, Hongzhuan Chen, Xiaoling Gao
Summary: The presence of protein corona on the surface of nanoparticles plays a role in modulating their interactions with cells, and proteomic analysis provides insights into the composition of the corona and its association with cellular uptake of nanoparticles.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Engineering, Biomedical
Magdalena Jurczyk, Monika Musial-Kulik, Aleksander Forys, Marcin Godzierz, Bozena Kaczmarczyk, Janusz Kasperczyk, Dorota Wrzesniok, Artur Beberok, Katarzyna Jelonek
Summary: The interest in combining chemosensitizers with cytostatics in cancer therapy is growing, which requires the development of efficient drug delivery systems. This study evaluates the effect of the poly(lactide) block on the co-encapsulation and release rate of docetaxel and resveratrol. The findings provide valuable information for the design of poly(lactide)-based drug delivery systems containing both drugs.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Polymer Science
Ewa Rybak, Piotr Kowalczyk, Sylwia Czarnocka-Sniadala, Michal Wojasinski, Jakub Trzcinski, Tomasz Ciach
Summary: The nanoprecipitation method was used to successfully formulate fluorescent PCL nanoparticles with small average diameter and low polydispersity index. In vitro experiments showed controlled dye release from these nanoparticles. Furthermore, MTT assays confirmed the non-toxicity of the PCL nanoparticles to cells. Thus, PCL nanoparticles have the potential for developing new drug delivery systems.
Article
Pharmacology & Pharmacy
M. Brzezinski, W. Gonciarz, B. Kost, M. Chmiela
Summary: Histamine is involved in neurotransmission, inflammatory response, and immunity. It can enhance drug-loaded nanoparticles distribution, but the risk of deleterious effects limits its delivery. In this study, histamine was used as an initiator for lactide polymerization to prepare doxorubicin-loaded nanoparticles, which showed enhanced cytotoxicity towards gastric cancer cells and reduced production of ZO-1 protein. This strategy may lead to effective, targeted, and low-toxic delivery of drugs into cancer cells.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Viktoriya Sokolova, Jana-Fabienne Ebel, Sebastian Kollenda, Kai Klein, Benedikt Kruse, Claudia Veltkamp, Christian M. Lange, Astrid M. Westendorf, Matthias Epple
Summary: Ultrasmall gold nanoparticles are capable of penetrating intestinal cells, colorectal cancer cells, and related 3D structures, while dissolved dyes cannot be taken up by these cells or structures. The cellular uptake of ultrasmall gold nanoparticles is found to occur through different endocytosis pathways. The nanoparticles conjugated with the cytostatic drug doxorubicin exhibit enhanced cytotoxicity against tumor spheroids.
Article
Toxicology
Mi Ho Jeong, Yubin Han, In-Sun Oh, Dong Min Kim, Dong-Won Son, Mi-Sook Jung, Hyoseon Yang, Kyuhong Lee, Ju-Young Shin, Ha Ryong Kim, Kyu Hyuck Chung
Summary: This study evaluated the transferability, reproducibility, and predictive capacity of an in vitro acute inhalation toxicity test method, showing good consistency within and between laboratories and reliability and relevance in predicting the acute toxicity of inhalable chemicals.
TOXICOLOGY IN VITRO
(2021)
Article
Chemistry, Multidisciplinary
Yun Chen, Liying Wang, Weishuo Chen, Channa Zhong, Shuangcheng Li, Jun Shao, Gao Li, Haoqing Hou
Summary: This study investigated the crystallization kinetics of PLLA and PLLA blocks in PEG-PLLA copolymers with different block lengths. The results showed that the crystallization rate of the PLLA block strongly depends on the copolymer architecture and block length. The crystallization rate first decreased and then increased with the increase in crystallization temperature (Tc). Moreover, higher crystallization rates were observed at lower Tc values and for copolymers with longer PEG block lengths.
Article
Nanoscience & Nanotechnology
Samurdhi A. Wijesundera, Kalana W. Jayawardana, Mingdi Yan
Summary: Trehalose-functionalized silver nanoparticles (AgNP-Tre) effectively reduce cytotoxicity while maintaining antimicrobial activity, possibly by slowing down silver release and enhancing interactions with mycobacteria.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Medicinal
Samriti Rana, Joga Singh, Aishani Wadhawan, Archna Khanna, Gurpal Singh, Mary Chatterjee
Summary: This study evaluated the toxicological profile of biosurfactant encapsulated polymeric nanoparticles in mice, showing no significant toxicological effects and confirming the safety of the treatment.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Enkeleda Meziu, Kristela Shehu, Marcus Koch, Marc Schneider, Annette Kraegeloh
Summary: This study investigates the impact of mucus modulation on its protective role and examines the enhanced penetration of particles through mucus with NAC treatment. The findings highlight the importance of intact mucus in protecting the underlying epithelium from particle-induced effects.
INTERNATIONAL JOURNAL OF PHARMACEUTICS-X
(2023)
Article
Chemistry, Physical
Mareike F. S. Deuker, Volker Mailaender, Svenja Morsbach, Katharina Landfester
Summary: Poly(ethylene glycol) (PEG) is commonly used to reduce unspecific protein adsorption and prolong nanocarrier circulation time, but the presence of anti-PEG antibodies in the bloodstream can counteract its stealth effect. This study found a high concentration and prevalence of anti-PEG antibodies in the German population, which led to higher uptake in macrophages and accelerated blood clearance of PEGylated nanocarriers.
NANOSCALE HORIZONS
(2023)
Article
Biology
Heng Yang, Junsik Sung, Zahra Alghoul, Didier Merlinz
Summary: Oral administration of colon-targeting nanoformulations has many advantages in the treatment of ulcerative colitis. This article presents a detailed protocol for the construction of a colon-targeting PLGA/PLA-PEG-FA nanoformulation and demonstrates its visualization of cell uptake in vitro.
Article
Chemistry, Multidisciplinary
Maria P. Meivita, Shao-Xiang Go, Fitya S. Mozar, Lunna Li, Yaw Sing Tan, Natasa Bajalovic, Desmond K. Loke
Summary: Biomarkers have the potential to be utilized in disease diagnosis, prediction, and monitoring, with the cancer cell type being a leading candidate for next-generation biomarkers. A sensor system based on phages has been developed, which can detect clinically relevant MCF-7 cells with high specificity, rapidity, and sensitivity.
Article
Chemistry, Multidisciplinary
Maria P. Meivita, Shao-Xiang Go, Fitya S. Mozar, Lunna Li, Yaw Sing Tan, Natasa Bajalovic, Desmond K. Loke
Summary: Biomarkers have the potential to be utilized in disease diagnosis, prediction and monitoring. This study developed a Phage-based Digital Biomolecular Sensor (P-DBS) for specific, rapid and sensitive detection of clinically-relevant MCF-7 cells. By utilizing a combination of Phage-integrated Polymer and Nanosheet (PPN) technology to control the electrical signature of tungsten disulfide (WS2), the P-DBS achieved efficient detection of low cell-population cancer cell types.
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)