Article
Biochemistry & Molecular Biology
Alex J. Anderson, Emerson Grey, Nicholas J. Bongiardina, Christopher N. Bowman, Stephanie J. Bryant
Summary: A new particulate delivery system based on step-growth thiol-Michael dispersion polymerization was reported in this study, in which synthetic nucleic acid mimic click nucleic acids (CNA) were functionalized onto low polydispersity microparticles. The results showed that CNA-functionalized microparticles exhibited controllable DNA loading and release properties, suggesting a promising platform for controlled DNA delivery.
Article
Pharmacology & Pharmacy
Eivina Radzeviciute, Veronika Malysko-Ptasinske, Jurij Novickij, Vitalij Novickij, Irute Girkontaite
Summary: In this study, the researchers transferred protein-encoding plasmids into different types of cells using sub-microsecond pulsed electric fields. They found that nanosecond pulses resulted in higher transfection efficiency, and the size and concentration of the plasmids also affected the transfection efficiency. Additionally, there was no significant difference in transfection efficiency between immature and mature immune cells.
Review
Biochemical Research Methods
Andrew Hamann, Angela K. Pannier
Summary: Although human mesenchymal stem cells (hMSCs) have not yet been approved by the FDA for clinical use, research is ongoing to develop gene delivery methods for therapeutic applications. Strategies, such as innovative carriers and chemical and physical priming, have been developed to improve transfection efficiency in hMSCs. These strategies aim to optimize cell phenotypes for enhanced immunomodulation and tissue regeneration, as well as for functions like tumor-killing and tissue engineering.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Diviya Santhanes, Alex Wilkins, Huiming Zhang, Robert John Aitken, Mingtao Liang
Summary: Lipid/polymer hybrid nanoparticles loaded with red fluorescent protein encoded plasmid DNA were successfully formulated using microfluidics. These nanoparticles showed high encapsulation efficiency and transfection efficacy, demonstrating their potential for clinical applications in DNA therapeutics.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Eivina Radzeviciute-Valciuke, Jovita Gecaite, Augustinas Zelvys, Aukse Zinkeviciene, Rokas Zalneravicius, Veronika Malysko-Ptasinske, Ausra Nemeikaite-Ceniene, Vytautas Kaseta, Natalija German, Jurij Novickij, Almira Ramanaviciene, Julita Kulbacka, Vitalij Novickij
Summary: This study demonstrates that MHz frequency bursts of nanosecond pulses can enhance gene delivery and investigates the potential of gold nanoparticles in this process. The combination of microsecond pulses and gold nanoparticles significantly improves gene delivery, depending on the surface charge and size of the nanoparticles. Gold nanoparticles are ineffective in nanosecond pulse treatment, but MHz pulse treatment remains competitive, with high gene delivery efficiency, low reactive oxygen species generation, preserved cellular viability, and easier operation.
Review
Pharmacology & Pharmacy
Shintaro Fumoto, Tsuyoshi Yamamoto, Kazuya Okami, Yuina Maemura, Chisato Terada, Asako Yamayoshi, Koyo Nishida
Summary: Nucleic acid and genetic medicines show great potential in treating various intractable diseases. Understanding their in vivo fate is crucial for rational design and development of drugs. Plasma protein binding can be utilized to manipulate pharmacokinetics, while ligand conjugation method is widely used for controlling drug distribution in the body.
Article
Pharmacology & Pharmacy
C. Bellefroid, C. Reusch, A. Lechanteur, B. Evrard, F. Debacq-Chainiaux, D. Mottet, G. Piel
Summary: This study aimed to develop a suitable gene delivery formulation for topical use on skin, and found that different lipid combinations can effectively deliver plasmid DNA into skin cells. Certain formulations showed high transfection rates with limited cytotoxicity, presenting promising potential for gene therapy in treating skin diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Treniece L. Terry, Brittany E. Givens, Andrea Adamcakova-Dodd, Peter S. Thorne, Victor G. J. Rodgers, Aliasger K. Salem
Summary: Encapsulating genetic material into biocompatible polymeric microparticles can improve gene transfection efficiency and reduce inflammatory responses. Factors such as the N/P ratio of PEI to DNA and PEGylation play roles in enhancing transgene expression and reducing inflammation. The study demonstrated the advantages of PEGylation and lack of porosity in microparticles for faster release of genetic cargo and decreased inflammatory response.
Article
Materials Science, Biomaterials
Randa Zoqlam, Chris J. Morris, Mohammad Akbar, Alaaldin M. Alkilany, Sherif I. Hamdallah, Peter Belton, Sheng Qi
Summary: An effective delivery vehicle is crucial for successful gene therapy, with nanoparticles often being used as the preferred choice. Despite claims of superior properties of microfluidic-prepared nanoparticles, direct comparisons with conventional methods are lacking. This study compared DNA-loaded nanoparticles prepared by microfluidic and conventional methods, finding differences in size and DNA loading efficiency. While both methods showed low cytotoxicity, conventional preparation showed higher transfection efficiency, indicating potential for plasmid delivery.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Medicine, Research & Experimental
Jaidev Chakka, Mohammed Maniruzzaman
Summary: In this study, a novel extrusion-based Sprayed Multi Adsorbed-droplet Reposing Technology (SMART) was used to prepare 150 nm plasmid DNA nanoparticles, which were successfully applied in vitro transfection experiments with cancer cells.
MOLECULAR PHARMACEUTICS
(2023)
Article
Multidisciplinary Sciences
Yuting Huang, Robert L. Kruse, Hui Ding, Mohamad I. Itani, Jonathan Morrison, Zack Z. Wang, Florin M. Selaru, Vivek Kumbhari
Summary: The study conducted hydrodynamic injections in pigs and found that they could tolerate multiple injections without major organ damage. By adjusting injection parameters, suitable parameters for human-sized animal models were identified. Future investigations will explore the impact of higher flow rates and pressure on DNA delivery efficiency.
Editorial Material
Multidisciplinary Sciences
Roberto Di Blasi, Masue M. Marbiah, Velia Siciliano, Karen Polizzi, Francesca Ceroni
Summary: Transient transfections are commonly used in basic and synthetic biology studies to investigate pathway regulation and circuit design, but resource competition for gene expression can lead to biases in data interpretation, emphasizing the need for better transfection controls to ensure reliable results.
NATURE COMMUNICATIONS
(2021)
Article
Polymer Science
Vera Chernonosova, Marianna Khlebnikova, Victoriya Popova, Ekaterina Starostina, Elena Kiseleva, Boris Chelobanov, Ren Kvon, Elena Dmitrienko, Pavel Laktionov
Summary: Biomaterial-mediated, spatially localized gene delivery is important for tissue engineering. This study investigated the release of pDNA from electrospun scaffolds loaded with silica nanoparticles and the interaction between cells and scaffolds. It was found that the size of the nanoparticles was suitable for cell transfection and the pDNA-loaded scaffolds did not have significant cytotoxicity.
Article
Biochemistry & Molecular Biology
Safa A. Damiati, Samar Damiati
Summary: The study combines microfluidic and artificial intelligence technologies, alongside biomaterials, to generate drug-loaded polymeric microparticles. By optimizing conditions, successfully produced IND-loaded PLGA particles were created. This technology offers a quick, low-cost method for producing particles with controlled shape and size.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Lyubov Agafonova, Dmitry Zhdanov, Yulia Gladilina, Sergey Kanashenko, Victoria Shumyantseva
Summary: An electrochemical approach for assessing transient DNA transfection efficiency in eukaryotic cells was proposed and demonstrated significant differences in behavior between transfected and control cells using differential pulse voltammetry. This method allows registration of 4000 cells per electrode and can be used for incorporating therapeutic genes into cells without the use of reporter genes.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Ling Ding, Siyuan Tang, Ao Yu, Anlin Wang, Weimin Tang, Huizhen Jia, David Oupicky
Summary: Pancreatic cancer progression relies on the nervous tumor microenvironment. This study utilized cationic perfluorocarbon nanoemulsions to deliver therapeutic siRNA and silence nerve growth factor (NGF) expression in orthotopic pancreatic tumors. The nanoemulsions achieved deep tumor penetration, enhanced NGF gene silencing, and effectively suppressed tumor growth in vivo and in vitro compared to control polycation/siRNA polyplexes. Delivery of NGF siRNA using nanoemulsion is a promising treatment approach for pancreatic cancer by targeting the tumor-neuronal interactions.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Ling Ding, Siyuan Tang, Weimin Tang, Deanna D. Mosley, Ao Yu, Diptesh Sil, Svetlana Romanova, Kristina L. Bailey, Daren L. Knoell, Todd A. Wyatt, David Oupicky
Summary: This study reports a method using PFOB nanoemulsions to improve the delivery efficiency of siRNA in the treatment of pulmonary fibrosis. The results show that the nanoemulsions can effectively treat pulmonary fibrosis and inhibit the progression of the disease.
Article
Biochemistry & Molecular Biology
Weimin Tang, Sudipta Panja, Chinmay M. Jogdeo, Siyuan Tang, Ao Yu, David Oupicky
Summary: This study investigates the impact of charge and hydrophilicity of polymers on their accumulation in injured kidneys and demonstrates that polymers with hydroxyl group modification show the least accumulation. These findings provide important guidance for the development of targeted drug carriers for AKI therapy.
Article
Engineering, Biomedical
Weimin Tang, Sudipta Panja, Chinmay M. Jogdeo, Siyuan Tang, Ling Ding, Ao Yu, Kirk W. Foster, Del L. Dsouza, Yashpal S. Chhonker, Heather Jensen-Smith, Hee-Seong Jang, Erika I. Boesen, Daryl J. Murry, Babu Padanilam, David Oupicky
Summary: This study demonstrates the synthesis of a novel siRNA carrier, C-CS, which shows potential for targeted delivery to injured kidneys. The C-CS/siRNA nanoparticles effectively accumulate and deliver therapeutic siRNAs to injured kidneys through CXCR4 binding, providing a new approach for AKI therapy.
Article
Chemistry, Multidisciplinary
Devika S. Manickam
Summary: The field of drug delivery has made significant advances in enhancing the potential of various types of drug candidates. Extracellular vesicles (EVs) are a promising class of natural, cell-derived carriers for drug delivery. Medium-to-large EVs can carry healthy and functional mitochondria, repair cellular damage, and have broad application prospects in the treatment of neurodegenerative diseases, cardiovascular diseases, and metabolic diseases.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biochemistry & Molecular Biology
Diptesh Sil, Sudipta Panja, Chinmay M. Jogdeo, Raj Kumar, Ao Yu, Cassandra E. Holbert, Ling Ding, Jackson R. Foley, Tracy Murray Stewart, Robert A. Casero, David Oupicky
Summary: The PG11047 analogs HPGs showed enhanced anticancer activity in various cancer cell lines, with the ability to self-assemble and form nanoparticles.
Article
Pharmacology & Pharmacy
Chuhan Zhang, Yu Hang, Weimin Tang, Diptesh Sil, Heather C. Jensen-Smith, Robert G. Bennett, Benita L. McVicker, David Oupicky
Summary: This study developed dual-functioning nanoparticles for the effective delivery of antifibrotic RNA and CXCR4 inhibitor, which shows promise for improving the treatment of alcohol-associated liver disease. The nanoparticles were able to significantly silence miR-155 expression and reduce liver damage in a model.
Article
Engineering, Biomedical
Huizhen Jia, Ling Ding, Ao Yu, Weimin Tang, Siyuan Tang, Chuhan Zhang, David Oupicky
Summary: Crosstalk between Kupffer cells (KCs) and hepatic stellate cells (HSCs) is important in liver fibrosis, and inhibiting this crosstalk can reverse alcohol-associated liver disease (AALD) fibrosis. In this study, a multifunctional nanosystem was designed to block the crosstalk by downregulating miR-155 expression in KCs and inhibiting CXCR4 signaling in activated HSCs. The combination of these inhibitions improved antifibrosis effects in an AALD fibrosis mouse model.
BIOACTIVE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Kandarp M. Dave, Duncan X. Dobbins, Maura N. Farinelli, Abigail Sullivan, Jadranka Milosevic, Donna B. Stolz, Jeongyun Kim, Siyang Zheng, Devika S. Manickam
Summary: The study demonstrates that pharmacological modulation of mitochondrial biogenesis in donor cells can increase the mitochondrial load in secreted extracellular vesicles (EVs). Mitochondria-enriched EVs (mito-EVs) show better effects in increasing recipient cell ATP levels and improving mitochondrial function in neurovascular and neurodegenerative diseases compared to naive EVs.
CELLULAR AND MOLECULAR BIOENGINEERING
(2022)
Article
Pharmacology & Pharmacy
Zheng-Hong Peng, Chinmay M. Jogdeo, Jing Li, Ying Xie, Yazhe Wang, Yuri M. Sheinin, Jindrich Kopecek, David Oupicky
Summary: Significant progress has been made in nanomedicine for cancer treatment, but there is relatively less research on managing tumor metastasis. This study introduces a polymeric conjugate P-DOX-iRGD that not only inhibits primary tumor growth but also substantially inhibits pulmonary metastasis in a breast cancer mouse model. Furthermore, treatment with P-DOX-iRGD reduces splenomegaly and liver hematopoiesis caused by breast cancer.
Article
Medicine, Research & Experimental
Rubayat Khan, Sudipta Panja, Ling Ding, Siyuan Tang, Weimin Tang, Ekta Kapoor, Robert G. Bennett, David Oupicky
Summary: Polymeric HCQ (PCQ) has shown to efficiently inhibit cancer cell migration and colony formation, and has strong antimetastatic activity in a pancreatic cancer model, suggesting its potential as an adjuvant therapy for pancreatic cancer.
MOLECULAR PHARMACEUTICS
(2022)
Review
Engineering, Biomedical
Chinmay M. Jogdeo, Sudipta Panja, Shrey Kanvinde, Ekta Kapoor, Kasturi Siddhanta, David Oupicky
Summary: Combination therapy is effective in cancer and inflammatory diseases, and lipid-based codelivery systems have attracted attention due to their low toxicity, biocompatibility, and ease of formulation scale-up.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Oncology
Ravi M. Raghani, Jeffrey A. Ma, Yining Zhang, Sophia M. Orbach, Jing Wang, Mina Zeinali, Sunitha Nagrath, Sandeep Kakade, Qichen Xu, Joseph R. Podojil, Tushar Murthy, Adam Elhofy, Jacqueline S. Jeruss, Lonnie D. Shea
Summary: Research has found that intravenous administration of cargo-free polymeric nanoparticles (NPs) can modify the immune environment in the lungs, reducing pulmonary metastases of triple-negative breast cancer. The administration of NPs alters the phenotype and function of myeloid cells in the lungs, promoting anti-tumor and inflammatory responses. The reduction of metastases by NPs is dependent on functional T-cells.
FRONTIERS IN ONCOLOGY
(2022)
Meeting Abstract
Immunology
Samantha Genardi, Joseph R. Podojil, Sandeep Kakade, Michael Boyne, Adam Elhofy, Stephen D. Miller
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Joseph R. Podojil, Samantha Genardi, Ming-Yi Chiang, Sandeep Kakade, Tobias Neef, Tushar Murthy, Michael T. Boyne, Adam Elhofy, Stephen D. Miller
Summary: Type 1 diabetes is an autoimmune disease that can be effectively inhibited by encapsulating multiple diabetogenic proteins in nanoparticles, which induce regulatory T cells and decrease inflammation in pancreatic islets.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
