期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 405, 期 C, 页码 84-93出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2015.01.042
关键词
PRDM16; Adipogenesis; Human adipose tissue; Obesity; Insulin sensitivity
资金
- Institut de Salud Carlos III from Spain [FIS-PI11/00214, FIS-PI12/02631]
- Fondo Europeo de Desarrollo Regional (FEDER) from Spain
In the present study, we aimed to evaluate the possible role of PRDM16 in human adipocytes and in whole adipose tissue according to obesity and insulin sensitivity. PRDM16 knockdown (KD) had a dual behavior. While KD in preadipocytes led to enhanced gene expression markers of adipocyte differentiation, PRDM16 KD in fully differentiated adipocytes resulted in decreased adipogenic gene expression and insulin action. In line with KD in adipocytes, PRDM16 was positively associated with the expression of several genes involved in adipogenesis, insulin signaling, mitochondrial function and brown adipocyte-related markers in whole adipose tissue from two independent cohorts. PRDM16 was decreased in obese subjects in relation with the decrease of insulin sensitivity [HOMA(IR) (cohort 1) and M clamp value (cohort 2)]. Rosiglitazone (5 mu mol/1) and metformin (5 mmo1/1) led to increased PRDM16 mRNA and protein levels in isolated human adipocytes and in whole adipose tissue. In conclusion, PRDM16 might contribute to maintain adipose tissue white fat gene expression profile and systemic metabolic homeostasis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据