期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 406, 期 C, 页码 1-9出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2015.02.009
关键词
Human adipose-derived stem cells; Arginine vasopressin; Ca2+ signaling; Differentiation
资金
- NIH HHS [P51 OD011104] Funding Source: Medline
Intracellular Ca2+ signaling is important for stem cell differentiation and there is evidence it may coordinate the process. Arginine vasopressin (AVP) is a neuropeptide hormone secreted mostly from the posterior pituitary gland and increases Ca2+ signals mainly via V1 receptors. However, the role of AVP in adipogenesis of human adipose-derived stem cells (hASCs) is unknown. In this study, we identified the V1a receptor gene in hASCs and demonstrated that AVP stimulation increased intracellular Ca2+ concentration during adipogenesis. This effect was mediated via Via receptors, Gq-proteins and the PLC-IP3 pathway. These Ca2+ signals were due to endoplasmic reticulum release and influx from the extracellular space. Furthermore, AVP supplementation to the adipogenic medium decreased the number of adipocytes and adipocyte marker genes during differentiation. The effect of AVP on adipocyte formation was reversed by the Via receptor blocker V2255. These findings suggested that AVP may function to inhibit adipocyte differentiation. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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