期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 403, 期 1-2, 页码 61-71出版社
SPRINGER
DOI: 10.1007/s11010-015-2337-y
关键词
Pink-eyed dilution (P) protein; Melanosome; siRNA transfection; Melanin; Transmission electron microscopy
类别
资金
- Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea [HN10C0034]
The pink-eyed dilution protein (P-protein) plays a critical role in melanin synthesis in melanocytes and retinal pigment epithelium cells. Mutation in this protein may cause complete or partial albinism. Role of the P-protein ranges in melanin synthesis to maturation and trafficking of the melanosomes. The aim of the present study was to evaluate the effect of P-protein inhibition on melanosome biology by comparing the shape, size, count, and types of melanosomes in melan-a melanocytes. The cells were extensively examined by the transmission electron microscopy. The P-protein inhibition was carried by P-protein-siRNA transfection to melan-a melanocytes, B16F10 mouse melanoma, and melan-p1 cells. Measurement of melanin contents, cellular tyrosinase, and different tyrosinase related proteins were also determined to investigate the effect of P-protein siRNA transfection on melanocytes. Results suggested that the inhibition of P-protein can significantly change the melanosomal morphology, types and their respective numbers, and provided a novel strategy for the control of melanin synthesis.
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