期刊
MOLECULAR & CELLULAR PROTEOMICS
卷 14, 期 9, 页码 2301-2307出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.O114.047035
关键词
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资金
- National Institutes of Health [R01 GM103551, R01 GM096306, P41 GM103533, R21 CA192983, F31 AG037265]
- US Department of Energy
- European Research Council [ERC-2008-AdG 233226]
In mass spectrometry-based bottom-up proteomics, data-independent acquisition is an emerging technique because of its comprehensive and unbiased sampling of precursor ions. However, current data-independent acquisition methods use wide precursor isolation windows, resulting in cofragmentation and complex mixture spectra. Thus, conventional database searching tools that identify peptides by interpreting individual tandem MS spectra are inherently limited in analyzing data-independent acquisition data. Here we discuss an alternative approach, peptide-centric analysis, which tests directly for the presence and absence of query peptides. We discuss how peptide-centric analysis resolves some limitations of traditional spectrum-centric analysis, and we outline the unique characteristics of peptide-centric analysis in general.
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